| Definition | Burkholderia cenocepacia HI2424 chromosome 1, complete sequence. |
|---|---|
| Accession | NC_008542 |
| Length | 3,483,902 |
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The map label for this gene is cca [H]
Identifier: 116691049
GI number: 116691049
Start: 3344039
End: 3345280
Strand: Reverse
Name: cca [H]
Synonym: Bcen2424_3031
Alternate gene names: 116691049
Gene position: 3345280-3344039 (Counterclockwise)
Preceding gene: 116691050
Following gene: 116691047
Centisome position: 96.02
GC content: 70.45
Gene sequence:
>1242_bases ATGAACATCTACGCAGTAGGCGGAGCGATCCGCGACGAATTGCTCGGCGTGCCGGTGCAGGACCGCGACTACGTGGTGGT CGGCGCGACGCCCGAGCAGATGACCGCGCAGGGCTTCCGGGCGGTCGGCAAGGATTTCCCGGTGTTCCTGCATCCGCAGA CGCAGGAGGAGTACGCGCTGGCGCGCACCGAGCGCAAGACGGCGGCCGGCTATCACGGCTTCCAGTTCCACTACGCGCCG GACGTGACGCTCGACGAGGATCTCGCGCGGCGCGACCTGACGATCAACGCGATGGCGCGCGAGGTGAGCCCGGAAGGCGC GCTGGTCGGCCCGGTGATCGATCCGTTCGACGGGCAGGCCGACCTGCGTGCGCGTGTGTTCCGGCACGTGAGCGACGCAT TCGTCGAGGACCCGGTGCGAATCCTGCGCATCGCGCGCTTTGCCGCGCGGTTCGCGGATTTCACGGTGGCGGACGAAACG CTCGCGCTGATGCGACGGATGGTCGATGCGGGCGAGGTCGACGCGCTCGTGCCCGAACGCGTGTGGCAGGAAATCGCGCG CGGGTTGATGGAAGCGAAGCCGTCGCGGATGTTCGCGGTGCTGCGCGACTGCGGCGCGCTCGCGCGCATCCTGCCCGAGG TCGACGCGCTGTGGGGCGTGCCGCAGCGCGCCGATTACCACCCGGAAGTCGATACCGGCGTGCACGTGATGATGGTCGTC GACTACGCGGCGAAGCAGGGCTATTCGCTGGCGGTGCGCTTCGCGGCGCTCACGCACGATCTCGGCAAGGGGACGACGCC CGCGGACGTGCTGCCGCGCCACGTCGGCCACGAAAGCCGCAGCGTCGAGCTGCTCAAGCCGTTGTGCGAGCGGCTGCGCG TGCCGAACGAATGCCGCGATCTCGCGCTGGTGGTCGCGCGCGAGCACGGCAACCTGCATCGCGTGATGGAGATGGGCGCG GCCGCGCTGGTGCGGCTGTTCGAGCGCAGCGACGCGCTGCGCAAGCCGGCGCGCTTCGCCGAGTTGCTCCAGGCCTGCGA ATCGGATGCGCGCGGCCGGCTCGGGCTCGATGCGCAGCCGTATCCGCAGGCCGAGCGGCTGCGCGTCGCGCTCGCGGCGG CGCGCAGTGTCGACGCCGGCGCGATCGCACGCGGCATCGGCAACGATACGGAGAAGATAAAGGAAGCCGTGCATCGCGCG CGGATCCAGGCCGTCGCGCAGGCGCTCGCGATCGGCGAGTAG
Upstream 100 bases:
>100_bases CCGGCTACGCGGCGCGGGTGACCGCGCTGCCGGCCGTGCAGCAGTGGATCGATGCCGCGCGCCGTGAAACCAACGTGATC GCCGAATACGAGCCGCAGCC
Downstream 100 bases:
>100_bases TCGGCAAGTAGCAGGCAAGTAGCAGGCGCGGAACAGCGTGCGCGCCGGCCAGCCGTTACGGTTTCTTCTTGCGCGCCGGA GTCGCGGCCGCCGGGCGTTT
Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase
Products: NA
Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]
Number of amino acids: Translated: 413; Mature: 413
Protein sequence:
>413_residues MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMTAQGFRAVGKDFPVFLHPQTQEEYALARTERKTAAGYHGFQFHYAP DVTLDEDLARRDLTINAMAREVSPEGALVGPVIDPFDGQADLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADET LALMRRMVDAGEVDALVPERVWQEIARGLMEAKPSRMFAVLRDCGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV DYAAKQGYSLAVRFAALTHDLGKGTTPADVLPRHVGHESRSVELLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA AALVRLFERSDALRKPARFAELLQACESDARGRLGLDAQPYPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRA RIQAVAQALAIGE
Sequences:
>Translated_413_residues MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMTAQGFRAVGKDFPVFLHPQTQEEYALARTERKTAAGYHGFQFHYAP DVTLDEDLARRDLTINAMAREVSPEGALVGPVIDPFDGQADLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADET LALMRRMVDAGEVDALVPERVWQEIARGLMEAKPSRMFAVLRDCGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV DYAAKQGYSLAVRFAALTHDLGKGTTPADVLPRHVGHESRSVELLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA AALVRLFERSDALRKPARFAELLQACESDARGRLGLDAQPYPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRA RIQAVAQALAIGE >Mature_413_residues MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMTAQGFRAVGKDFPVFLHPQTQEEYALARTERKTAAGYHGFQFHYAP DVTLDEDLARRDLTINAMAREVSPEGALVGPVIDPFDGQADLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADET LALMRRMVDAGEVDALVPERVWQEIARGLMEAKPSRMFAVLRDCGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV DYAAKQGYSLAVRFAALTHDLGKGTTPADVLPRHVGHESRSVELLKPLCERLRVPNECRDLALVVAREHGNLHRVMEMGA AALVRLFERSDALRKPARFAELLQACESDARGRLGLDAQPYPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRA RIQAVAQALAIGE
Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing
COG id: COG0617
COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]
Homologues:
Organism=Escherichia coli, GI1789436, Length=408, Percent_Identity=53.6764705882353, Blast_Score=430, Evalue=1e-122,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012006 - InterPro: IPR003607 - InterPro: IPR002646 [H]
Pfam domain/function: PF01743 PolyA_pol [H]
EC number: =2.7.7.21; =2.7.7.25 [H]
Molecular weight: Translated: 45490; Mature: 45490
Theoretical pI: Translated: 6.39; Mature: 6.39
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.0 %Cys (Translated Protein) 2.7 %Met (Translated Protein) 3.6 %Cys+Met (Translated Protein) 1.0 %Cys (Mature Protein) 2.7 %Met (Mature Protein) 3.6 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMTAQGFRAVGKDFPVFLHPQTQEEYAL CEEEEECCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTINAMAREVSPEGALVGPVIDPFDGQA HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCCH DLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADETLALMRRMVDAGEVDALVPER HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHH VWQEIARGLMEAKPSRMFAVLRDCGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE DYAAKQGYSLAVRFAALTHDLGKGTTPADVLPRHVGHESRSVELLKPLCERLRVPNECRD EHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH LALVVAREHGNLHRVMEMGAAALVRLFERSDALRKPARFAELLQACESDARGRLGLDAQP HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCC YPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRARIQAVAQALAIGE CCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MNIYAVGGAIRDELLGVPVQDRDYVVVGATPEQMTAQGFRAVGKDFPVFLHPQTQEEYAL CEEEEECCHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEECCCCHHHHHH ARTERKTAAGYHGFQFHYAPDVTLDEDLARRDLTINAMAREVSPEGALVGPVIDPFDGQA HHHHHHHHCCCCCEEEEECCCCCCCHHHHHHCCHHHHHHHHCCCCCCEECCCCCCCCCCH DLRARVFRHVSDAFVEDPVRILRIARFAARFADFTVADETLALMRRMVDAGEVDALVPER HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHH VWQEIARGLMEAKPSRMFAVLRDCGALARILPEVDALWGVPQRADYHPEVDTGVHVMMVV HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEE DYAAKQGYSLAVRFAALTHDLGKGTTPADVLPRHVGHESRSVELLKPLCERLRVPNECRD EHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHH LALVVAREHGNLHRVMEMGAAALVRLFERSDALRKPARFAELLQACESDARGRLGLDAQP HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCCCCC YPQAERLRVALAAARSVDAGAIARGIGNDTEKIKEAVHRARIQAVAQALAIGE CCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA