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The map label for this gene is puuA [H]

Identifier: 116691012

GI number: 116691012

Start: 3304199

End: 3305536

Strand: Direct

Name: puuA [H]

Synonym: Bcen2424_2994

Alternate gene names: 116691012

Gene position: 3304199-3305536 (Clockwise)

Preceding gene: 116691011

Following gene: 116691013

Centisome position: 94.84

GC content: 65.32

Gene sequence:

>1338_bases
ATGCAAGACATCGAAGACTTTCTGAAGCAGCATCGGATCACCGAGGTCGAGGCGATCATCCCCGACATGGCCGGCATCGC
GCGCGGCAAGATCACGCCGCGCAACAAGTTCACGTCCGGCGAATCGATGCGCCTGCCGCAAGCCGTGATGGTGCAGACCG
TTACCGGCGAATACCCGGAAGACGGCTCGCTCACCGGCGTGACCGACCCCGACATGGTGTGCGTGCCCGACACGTCGACG
ATCCGCCTGATCCCGTGGGCCGTGGACCCGACCGCGCAGGTGATCCACGATTGCGTGCACTTCGACGGCTCGCCGGTCGA
GATCTCGCCGCGCTACGTGCTGCGCCGCGTGCTCGACCTGTACAAGGCGAAGGGCTGGAAACCGGTCGTGGCGCCGGAGC
TCGAGTTCTACCTGGTCGACATGAACAAGGACCCCGACCTGCCGCTGCGTCCGCCGGTCGGCCGCACGGGCCGCCCCGAA
ACGGGCCGCCAGTCGTATTCGATCGAGGCCGTCAACGAATTCGATCCGCTGTTCGAGGACATCTACGAGTACTGCGAAGC
GCAGAACCTCGACATCGACACGCTGATCCACGAAGTCGGCGCCGCGCAGATGGAGATCAACTTCATGCACGGCGACGCGC
TGTCGCTGGCCGACCAGGTGTTCCTGTTCAAGCGCACGGTGCGCGAGGCCGCGCTGCGTCACAACATGTACGCGACGTTC
ATGGCCAAGCCGATGGAAGACGAACCGGGCTCGGCGATGCACGTGCACCAGAGCATCGTCGACGAGGAAACGGGGCAGAA
CCTGTTCACGTCGCCGGAGACCGGCGGCGCGACGTCGATGTTCTACAACTACCTCGCGGGGCTGCAGAAGTACACGCCGG
CGCTGATGCCGATCTTCGCGCCTTACATCAATTCGTACCGCCGCCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAG
TGGGGCTACGACAACCGCACGGTGGGGTTCCGCATTCCGCATTCGGGCCCGGCCGCGCGCCGCATCGAAAACCGCATCCC
GGGTGTCGACTGCAACCCGTACCTCGCGCTCGCGGCGACGCTCGCGGCCGGCTATCTCGGCATGACGCAGCGCCTGGAGC
CGACCGAGCCGCTGGTGAGCGACGGCTACAGCCTGCCGTACCAGCTGCCGCGCAACCTCGAGGAAGGGCTGACGCTGATG
GCCGCGTGCGAGCCGCTCGGCGAGATCCTCGGGCCCAAGTTCCTCAAGGCGTATTTCGCGCTGAAGGAAACCGAATACGA
AGCGTTCTTCCGCGTGATCAGCTCGTGGGAACGCCGCCATCTGCTGCTGCACGTTTAA

Upstream 100 bases:

>100_bases
GCATTCGGCGATGCCTGCCGCGCCCGCCGCGCCGCCCGCACGCGCGCCATGGCCGCCGCCGCGCTCGCCTGAGCGCGCCC
ACCAGACAACGAGAACAGAC

Downstream 100 bases:

>100_bases
GCGTGCCAACCGCATACGGAATCACGGAGGAAACATGACTTACCGCAACGAATCTGCCTGGGTCCAGCCGGCCGCGCCGA
GCGCCGCGGCCGCCGCGCCG

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MQDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDTST
IRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSPETGGATSMFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYSLPYQLPRNLEEGLTLM
AACEPLGEILGPKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_445_residues
MQDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDTST
IRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSPETGGATSMFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYSLPYQLPRNLEEGLTLM
AACEPLGEILGPKFLKAYFALKETEYEAFFRVISSWERRHLLLHV
>Mature_445_residues
MQDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPEDGSLTGVTDPDMVCVPDTST
IRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPE
TGRQSYSIEAVNEFDPLFEDIYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSPETGGATSMFYNYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQ
WGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAATLAAGYLGMTQRLEPTEPLVSDGYSLPYQLPRNLEEGLTLM
AACEPLGEILGPKFLKAYFALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=447, Percent_Identity=27.2930648769575, Blast_Score=151, Evalue=1e-36,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=34.1463414634146, Blast_Score=234, Evalue=8e-63,
Organism=Escherichia coli, GI1790301, Length=439, Percent_Identity=27.5626423690205, Blast_Score=138, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 50131; Mature: 50131

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
CCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DGSLTGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSPETGGATSMFYNYLAGLQKYTPALMPIFA
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQRLEPTEPLVSDGYSLPYQLPRNLEEGLTLMAACEPLGEILGPKFLKAYFA
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQDIEDFLKQHRITEVEAIIPDMAGIARGKITPRNKFTSGESMRLPQAVMVQTVTGEYPE
CCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCC
DGSLTGVTDPDMVCVPDTSTIRLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCCCCCCCCCCEEEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YKAKGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRPETGRQSYSIEAVNEFDPLFED
HHCCCCCCEECCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCEAQNLDIDTLIHEVGAAQMEINFMHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHCCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKPMEDEPGSAMHVHQSIVDEETGQNLFTSPETGGATSMFYNYLAGLQKYTPALMPIFA
HCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHH
PYINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSGPAARRIENRIPGVDCNPYLALAAT
HHHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHH
LAAGYLGMTQRLEPTEPLVSDGYSLPYQLPRNLEEGLTLMAACEPLGEILGPKFLKAYFA
HHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LKETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]