Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 116690970

GI number: 116690970

Start: 3264318

End: 3265238

Strand: Direct

Name: gcvA [H]

Synonym: Bcen2424_2952

Alternate gene names: 116690970

Gene position: 3264318-3265238 (Clockwise)

Preceding gene: 116690968

Following gene: 116690974

Centisome position: 93.7

GC content: 71.99

Gene sequence:

>921_bases
ATGCGACGCATCCACCTCCCTCCGCTGCAGACGCTGCGCGCGTTCGAAGCCGCCGTGCGGCTGCAGAGCTTCACACGCGC
GGCCGACGAACTCGCGCTCACGCAAGGCGCGGTCAGCCAGCACATTCGCGCGCTCGAGGCGCAGCTCGGCTATCCGCTGT
TCACGCGCGAACGCAACGGCGCGACGCCGACGCACGCCGCGCATGCCCTCGCACTGCAGGTTCGCCAAGGCCTGAGCGTG
CTCGAACGCGCGTTCGAGCCGACGCTGGCGGTGCGCCGCCGCCCGCGCACCTGCGACGTGACGCTGAACGTGAGCGTGCT
GCCGAGCGTCGCCGAGCGCTGGCTCGTGCCGCGCCTGCCGCGCTTCTCCGCCGCGCATCCGCACATCTCGATCGTGCTGC
ATCCGGACGTGGCGCTCGCGCCGCTGCGCAAACGCGACCGTATCGACGTCGCATTGCGCTACGGGCCCGGCACGTGGCCC
GGCGTCATCGCCGAGAAGCTGATGAACGAGACGATCTTCCCGGTCGCGAGCCCTGCATACCGCAATCGCGACGGCGTCGC
GCCGCGCACGGCGGCCGACCTCGTACGCGCGACGTTGCTGCGTCATCCCGCGCAGCCGTGGGAACCGTGGTGCCAGGCCG
CGCGGCTCGACCTCACCGAATCCGCGCGCGCGCCGCGCTTCACCGACACGAACACGCTGATCGACGCGGCACTGAAAGGC
CGCGGCGTCGCGCTCGCGCGCCGCTCGCTGATCGAGCCGGAACTCGCCGACGGCACGCTCGTGCGCGTGTCGACGGTACG
CATCACCGACGTGTACGCGCACTACGTCGTGTGGCGCCCCGGCCATCCGCACGAAGCCGCGATCCACACCTGGCTCGACT
GGCTGCGCAGCGAAGTGCGGCGCAGGACGCCGCGGCGCTGA

Upstream 100 bases:

>100_bases
CCGACGGGCTGTGGAAGACGGAACCGAGTGTAGGAACGCACGCCGATGCGCGGCAAACGATGAATTGTCGTGCTCGGACA
TTAGTGTTACTAATACCCGT

Downstream 100 bases:

>100_bases
CACCAATGCCGACGCCGACGCTCACGCGCGCATCGGCATCGCCACCTTGTCGAGCGTGATCGGCAGATCGCGCACCCGCA
CGCCGGTCGCGTGATAGACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNGATPTHAAHALALQVRQGLSV
LERAFEPTLAVRRRPRTCDVTLNVSVLPSVAERWLVPRLPRFSAAHPHISIVLHPDVALAPLRKRDRIDVALRYGPGTWP
GVIAEKLMNETIFPVASPAYRNRDGVAPRTAADLVRATLLRHPAQPWEPWCQAARLDLTESARAPRFTDTNTLIDAALKG
RGVALARRSLIEPELADGTLVRVSTVRITDVYAHYVVWRPGHPHEAAIHTWLDWLRSEVRRRTPRR

Sequences:

>Translated_306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNGATPTHAAHALALQVRQGLSV
LERAFEPTLAVRRRPRTCDVTLNVSVLPSVAERWLVPRLPRFSAAHPHISIVLHPDVALAPLRKRDRIDVALRYGPGTWP
GVIAEKLMNETIFPVASPAYRNRDGVAPRTAADLVRATLLRHPAQPWEPWCQAARLDLTESARAPRFTDTNTLIDAALKG
RGVALARRSLIEPELADGTLVRVSTVRITDVYAHYVVWRPGHPHEAAIHTWLDWLRSEVRRRTPRR
>Mature_306_residues
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNGATPTHAAHALALQVRQGLSV
LERAFEPTLAVRRRPRTCDVTLNVSVLPSVAERWLVPRLPRFSAAHPHISIVLHPDVALAPLRKRDRIDVALRYGPGTWP
GVIAEKLMNETIFPVASPAYRNRDGVAPRTAADLVRATLLRHPAQPWEPWCQAARLDLTESARAPRFTDTNTLIDAALKG
RGVALARRSLIEPELADGTLVRVSTVRITDVYAHYVVWRPGHPHEAAIHTWLDWLRSEVRRRTPRR

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=35.4948805460751, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1788706, Length=303, Percent_Identity=29.3729372937294, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1786448, Length=308, Percent_Identity=30.8441558441558, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI145693193, Length=206, Percent_Identity=33.0097087378641, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787589, Length=300, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788297, Length=170, Percent_Identity=28.8235294117647, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34391; Mature: 34391

Theoretical pI: Translated: 11.69; Mature: 11.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
ATPTHAAHALALQVRQGLSVLERAFEPTLAVRRRPRTCDVTLNVSVLPSVAERWLVPRLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCC
RFSAAHPHISIVLHPDVALAPLRKRDRIDVALRYGPGTWPGVIAEKLMNETIFPVASPAY
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGVAPRTAADLVRATLLRHPAQPWEPWCQAARLDLTESARAPRFTDTNTLIDAALKG
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCC
RGVALARRSLIEPELADGTLVRVSTVRITDVYAHYVVWRPGHPHEAAIHTWLDWLRSEVR
CCHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC
>Mature Secondary Structure
MRRIHLPPLQTLRAFEAAVRLQSFTRAADELALTQGAVSQHIRALEAQLGYPLFTRERNG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCC
ATPTHAAHALALQVRQGLSVLERAFEPTLAVRRRPRTCDVTLNVSVLPSVAERWLVPRLP
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCC
RFSAAHPHISIVLHPDVALAPLRKRDRIDVALRYGPGTWPGVIAEKLMNETIFPVASPAY
CCCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCC
RNRDGVAPRTAADLVRATLLRHPAQPWEPWCQAARLDLTESARAPRFTDTNTLIDAALKG
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHCC
RGVALARRSLIEPELADGTLVRVSTVRITDVYAHYVVWRPGHPHEAAIHTWLDWLRSEVR
CCHHHHHHHHCCCCCCCCEEEEEEEEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHH
RRTPRR
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]