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The map label for this gene is metH [H]

Identifier: 116690935

GI number: 116690935

Start: 3221366

End: 3224083

Strand: Direct

Name: metH [H]

Synonym: Bcen2424_2917

Alternate gene names: 116690935

Gene position: 3221366-3224083 (Clockwise)

Preceding gene: 116690934

Following gene: 116690937

Centisome position: 92.46

GC content: 65.45

Gene sequence:

>2718_bases
ATGACCGATCACATGATGCGCCTTGCCGGCCTCGAGCCGTTCAACGTCACGCCCGGGACGCTCTTCATCAACGTCGGCGA
ACGCACCAACGTCACCGGCTCGAAGGCGTTCGCACGGATGATCCTCAACGGCCAGTTCGACGAGGCGCTCGCCGTCGCGC
GCCAGCAGGTCGAGAACGGCGCGCAGGTGATCGACATCAACATGGACGAGGCGATGCTCGATTCGAAGGCGGCGATGGTG
CGCTTCCTGAACCTGATTGCGTCGGAGCCGGACATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGAGGTGAT
CGAAGCCGGCCTCAAGTGCGTGCAGGGCAAGGCGATCGTGAACTCGATCTCGCTGAAGGAAGGCGAGGACGCGTTCCGTC
ACCACGCGAACCTGATCCGCCGCTACGGCGCGGCCGCGGTCGTGATGGCGTTCGACGAAACCGGCCAGGCCGATACCTAC
GCGCGCAAGACCGAGATCTGCAAGCGCTCGTACGACTTCCTCGTGAACGAAGTCGGCTTCCCGCCGGAAGACATCATCTT
CGACCCGAACATCTTCGCGGTCGCGACCGGCATCGAGGAGCACAACAACTACGCGGTCGACTTCATCGAGGCGACCCGCT
GGATCAAGCAGAACCTGCCGCACGCGAAGGTGAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTCCGCGGCAACGACCCG
GTGCGCGAGGCGATCCACACCGTGTTCCTGTATCACGCGATCCAGGCCGGGATGGACATGGGCATCGTGAACGCGGGCCA
GCTCGGCGTGTACGCCGATCTCGACCCCGAACTGCGCGAGCGCGTCGAGGACGTGATCCTGAACCGCCGCGACGACTCCA
CCGATCGTCTGCTGGAGATCGCCGACAAGTTCAAGGCAGGCGGCGCGAAGAAGGAAGAGAACCTCGAGTGGCGCGACCAG
CCGGTCGAGAAGCGGCTCTCGCATGCGCTCGTGCACGGCATCACGACCTTCATCGTCGAGGATACCGAGGAAGTGCGCGC
GAAGATCGCCGCGGCCGGCGGCCGTCCGATCAACGTGATCGAAGGGCCGCTGATGGACGGGATGAACATCGTCGGCGACC
TGTTCGGCCAGGGCAAGATGTTCCTGCCGCAGGTCGTGAAGTCGGCGCGCGTGATGAAGCAGGCGGTCGCGCACCTGATC
CCGTTCATCGAGGAAGAAAAGCGCCTGCTCGCGGAAGCGGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTCATCGCGAC
CGTGAAGGGCGACGTGCACGACATCGGCAAGAACATCGTGTCGGTCGTGCTCCAGTGCAACAACTTCGAAGTGGTCAACA
TGGGCGTGATGGTCCCGTGCAACGAGATCCTCGCGAAGGCGAAGGTCGAAGGCGCGGACATCATCGGGCTGTCGGGCCTC
ATCACGCCGAGCCTCGAGGAAATGGCGTACGTCGCGTCCGAAATGCAGCGCGACGACTACTTCCGCGTGAAGAAGATTCC
GCTGCTGATCGGCGGCGCCACGACGTCGCGCGTGCACACGGCCGTGAAGATCGCGCCGCACTACGAAGGCCCGGTCGTCT
ACGTGCCGGATGCGTCGCGCTCGGTGTCGGTCGCGTCGAACCTGCTGTCCGACGAAGGCGCGGCGAAGTATCTGGACGAG
CTGAAGTCCGACTACGAGCGGATTCGCGACCAGCACGCGAACCGCAAGGCGCAGCCGGTGGTCACGCTCGCCGAAGCACG
CGCGAACAAGACCAAGGTCGACTGGGCCGGCTACCAGCCGGTGAAGCCGAAGTTCATCGGCCGCCGCGTGTTCAAGAATT
ACGACCTGAACGAGCTCGCGAACTACATCGACTGGGGCCCGTTCTTCCAGACCTGGGACCTCGCGGGCCCATACCCGGCG
ATCCTGAACGACGAGATCGTCGGCGAATCGGCGCGGCGCGTGTTCTCCGACGCGAAGTCGATGCTCGCGCGCCTGATCCA
GGGCCGCTGGCTGACCGCGAACGGCGTGATCGCGCTGCTGCCGGCCAATACGGTCAACGACGACGACATCGAGATCTACA
GCGACGAGTCACGCTCGGAAGTGCTGCTCACGTGGCGCAACCTGCGCCAGCAGAGCGTGCGCCCGGTGGTCGACGGCGTG
ATGCGGCCGAACCGTTCGCTCGCCGACTTCATCGCGCCGAAGGAGTCGGGGGTGGCCGACTACATCGGGATGTTCGCGGT
GACGGCCGGCCTCGGCGTCGACGTGAAGGAAAAGCAGTTCGAAGCCGACCACGACGACTACAGCGCGATCATGCTGAAGG
CGCTCGCCGACCGTTTCGCGGAAGCGTTCGCCGAAGCGATGCACGCGCGCGTGCGGCGCGAGCTGTGGGGCTATGCGAGC
GGCGAGACGCTCGACAACGACGCGCTGATCGCCGAGAAGTACGCGGGCATCCGCCCGGCGCCCGGCTATCCGGCTTGCCC
CGACCACCTCGTGAAGCGCGACATGTTCGACGTGCTGCACGCGGACGAGATCGGCATGAGCGTGACGGATTCGCTCGCGA
TGCTGCCGGCGGCGAGCGTGTCGGGTTTCTATCTCGCGCACCCGGACAGCCGCTACTTCTCGGTCGGCAAAATCGGGCAG
GATCAGCTCGAGGACTACGCGCAACGCATGGCGCTGTCGCTGGACGACGCGCGCCGCGCGCTCGCGCCGCAACTCTGA

Upstream 100 bases:

>100_bases
GGCGCTCGCCGACGTGAAGCCGCGCCGCTGGCCGAACCAGTACAGCGACAACGCCTGACCCGACCGCCCCACGTCCTTCA
TCAAAAGAATTCGCACCGCC

Downstream 100 bases:

>100_bases
TCGACGCAAACGGGCGGCCCGGCCGCCTTTTTATCAGACAAAAGAAAAAGCCCGCTCGAAAGCGGGCTTTTTCCTGTCTG
GCGAACCTTTCGATCTGCCA

Product: methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHMMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEDAFRHHANLIRRYGAAAVVMAFDETGQADTY
ARKTEICKRSYDFLVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPHAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRDQ
PVEKRLSHALVHGITTFIVEDTEEVRAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASNLLSDEGAAKYLDE
LKSDYERIRDQHANRKAQPVVTLAEARANKTKVDWAGYQPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVNDDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYAS
GETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLHADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQ
DQLEDYAQRMALSLDDARRALAPQL

Sequences:

>Translated_905_residues
MTDHMMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEDAFRHHANLIRRYGAAAVVMAFDETGQADTY
ARKTEICKRSYDFLVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPHAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRDQ
PVEKRLSHALVHGITTFIVEDTEEVRAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASNLLSDEGAAKYLDE
LKSDYERIRDQHANRKAQPVVTLAEARANKTKVDWAGYQPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVNDDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYAS
GETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLHADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQ
DQLEDYAQRMALSLDDARRALAPQL
>Mature_904_residues
TDHMMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVR
FLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIVNSISLKEGEDAFRHHANLIRRYGAAAVVMAFDETGQADTYA
RKTEICKRSYDFLVNEVGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPHAKVSGGVSNVSFSFRGNDPV
REAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEIADKFKAGGAKKEENLEWRDQP
VEKRLSHALVHGITTFIVEDTEEVRAKIAAAGGRPINVIEGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP
FIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGLI
TPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASRSVSVASNLLSDEGAAKYLDEL
KSDYERIRDQHANRKAQPVVTLAEARANKTKVDWAGYQPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAI
LNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVNDDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGVM
RPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFAEAFAEAMHARVRRELWGYASG
ETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLHADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQD
QLEDYAQRMALSLDDARRALAPQL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=917, Percent_Identity=54.525627044711, Blast_Score=974, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.2787610619469, Blast_Score=1100, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.2763157894737, Blast_Score=945, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 99914; Mature: 99783

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHMMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEDAFRHHANLIRRYGAAAVVMAFDETGQADTYARKTEICKRSYDFLVNEVGF
HHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPHAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKAGGAKKEENLEWRDQPVEKRLSHALVHGITTFIVEDTEEVRAKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC
SVSVASNLLSDEGAAKYLDELKSDYERIRDQHANRKAQPVVTLAEARANKTKVDWAGYQP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCCCCEEEECCCCC
VKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVIALLPANTVNDDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFA
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYASGETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQDQLEDYAQRMALSLDDARRA
HHHCCCHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAPQL
HCCCC
>Mature Secondary Structure 
TDHMMRLAGLEPFNVTPGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWEVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHHHCCHHHH
NSISLKEGEDAFRHHANLIRRYGAAAVVMAFDETGQADTYARKTEICKRSYDFLVNEVGF
HHCCCCCCHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPHAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCCCHHCCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYADLDPELRERVEDVILNRRDDSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKAGGAKKEENLEWRDQPVEKRLSHALVHGITTFIVEDTEEVRAKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEE
EGPLMDGMNIVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIIGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEEEECCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPHYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHHEEEEEEECCCCCCEEECCCCCC
SVSVASNLLSDEGAAKYLDELKSDYERIRDQHANRKAQPVVTLAEARANKTKVDWAGYQP
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHCCCCCEEEECCCCC
VKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVIALLPANTVNDDDIEIYSDESRSEVLLTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKESGVADYIGMFAVTAGLGVDVKEKQFEADHDDYSAIMLKALADRFA
HCCCCHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYASGETLDNDALIAEKYAGIRPAPGYPACPDHLVKRDMFDVLH
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
ADEIGMSVTDSLAMLPAASVSGFYLAHPDSRYFSVGKIGQDQLEDYAQRMALSLDDARRA
HHHCCCHHHHHHHHCCCCCCCCEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAPQL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]