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The map label for this gene is dctD [H]

Identifier: 116690891

GI number: 116690891

Start: 3170134

End: 3171486

Strand: Reverse

Name: dctD [H]

Synonym: Bcen2424_2871

Alternate gene names: 116690891

Gene position: 3171486-3170134 (Counterclockwise)

Preceding gene: 116690892

Following gene: 116690889

Centisome position: 91.03

GC content: 70.36

Gene sequence:

>1353_bases
ATGGCCAACCGGCTGCAAGTGATCTATATCGAAGACGATGCGCTCGTGCGCCGGGCGAGCGTGCAGAGCCTTCAACTGGC
GGGCTTCGACGTCGGCGGCTTCGAGTCGGCCGAGGCGGCCGAGAAGGCGATCGTCGCGGATACCGCCGGCGTGATCGTCA
GCGACATCCGACTGCCCGGCGCGAGCGGGCTCGACGTGCTCGCGCAGTGCCGCGAGCGCGTGCCGGACGTGCCGGTGATT
CTCGTCACCGGGCACGGCGACATTTCGATGGCCGTCCAGGCGATGCGCGACGGCGCGTACGACTTCATCGAAAAGCCGTT
CGCGGCCGAGCGCCTGATCGAGACGGTGCGCCGCGCGCTCGAGCGCCGCGAGCTCGTGCTCGAGAACCACGCGCTGCGGC
GCGAGCTGGCCGGGCAGAACGTGGTCGCGCCGCGCATCATCGGCCGCAGCCCCGCGATCGAGCAGGTGCGCAAGCTGATC
GCGAACGTCGCGCCCACCGACGCGTCGGTGCTGATCAACGGCGACACGGGCGCCGGCAAGGAACTGATCGCGCGCAGCCT
GCACGAGCTGTCGCCGCGCCGCGACAAGCCGTTCATCGCGGTCAATTGCGGCGCGCTGCCCGAGCCGATGTTCGAGTCGG
AGATGTTCGGCTACGAGCCCGGGGCGTTCACCGGTGCGGCGAAGCGGCGCGTCGGCAAGCTCGAATACGCGTCCGGCGGC
ACGCTGTTCCTCGACGAAATCGAAAGCATGCCGCTCGCGCTGCAGGTGAAGCTGTTGCGCGTGCTGCAGGACGGTGTGCT
GGAACGGCTCGGCTCGAACCAGCCGATCCGCGTGAACTGCCGCGTGGTCGCGGCCGCGAAGGGCGACATGAGCGAGCTCG
TCGCGGCCGGCACGTTCCGGCGCGACCTGCTGTACCGGCTCAACGTCGTGACGATCGCGCTGCCGCCGCTCGCCGAGCGC
CGCGAGGACATCGTGCCGCTGTTCGAGCACTTCATGCTCGATGCGGCCGTGCGCTACGGGCGGCCCGCGCCGGTGCTGAC
CGACCGGCAGCGCGCGAGCCTGATGCAGCGCGACTGGCCGGGCAACGTGCGCGAGCTGCGCAATGCGGCCGACCGCTTCG
TGCTCGGCGTGGCCGACATGCCGCCGGAAACGGGCGCGGGCGACGACGCCGACAACGACCAGACGCTGAAGGAGCGCATC
GAGCAGTTCGAGCGCGCGGTGATCGCCGAGGCGCTGAACCAGACGGGCGGCGCGGTCGCCGCGACGGCCGACCGGCTGCA
TGTCGGCAAGGCGACGCTATACGAGAAGATGAAGCGCTACGGGCTGTCGGCGAAAGGCGAAACCGAGCGCTGA

Upstream 100 bases:

>100_bases
GGGCTCGCATTAGCGTGAACAGGCCGGTCGACGAACGACCGGGATCAACGAGAGCCGCACGCACCAGCGCCGCGCGGCCT
GTCAGGAGCAAGTGACCAAG

Downstream 100 bases:

>100_bases
AAAGCAAACGGACCGCGGAACCGGGGTGCCCGGTCATGCGGTCCGTTGTTTTACCGAAAGAGGCGGTGCGGTGCGCGCTC
CTTCGAATCCGATTCGGCGG

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MANRLQVIYIEDDALVRRASVQSLQLAGFDVGGFESAEAAEKAIVADTAGVIVSDIRLPGASGLDVLAQCRERVPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPVLTDRQRASLMQRDWPGNVRELRNAADRFVLGVADMPPETGAGDDADNDQTLKERI
EQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGETER

Sequences:

>Translated_450_residues
MANRLQVIYIEDDALVRRASVQSLQLAGFDVGGFESAEAAEKAIVADTAGVIVSDIRLPGASGLDVLAQCRERVPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYGRPAPVLTDRQRASLMQRDWPGNVRELRNAADRFVLGVADMPPETGAGDDADNDQTLKERI
EQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGETER
>Mature_449_residues
ANRLQVIYIEDDALVRRASVQSLQLAGFDVGGFESAEAAEKAIVADTAGVIVSDIRLPGASGLDVLAQCRERVPDVPVIL
VTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIA
NVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGGT
LFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAERR
EDIVPLFEHFMLDAAVRYGRPAPVLTDRQRASLMQRDWPGNVRELRNAADRFVLGVADMPPETGAGDDADNDQTLKERIE
QFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGETER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=469, Percent_Identity=39.0191897654584, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.716814159292, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1790437, Length=447, Percent_Identity=37.5838926174497, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1788905, Length=445, Percent_Identity=36.6292134831461, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=42.1828908554572, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=39.6875, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1789087, Length=356, Percent_Identity=42.1348314606742, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI87082152, Length=319, Percent_Identity=42.9467084639498, Blast_Score=228, Evalue=7e-61,
Organism=Escherichia coli, GI87081872, Length=239, Percent_Identity=46.8619246861925, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1786524, Length=360, Percent_Identity=38.0555555555556, Blast_Score=214, Evalue=7e-57,
Organism=Escherichia coli, GI1787583, Length=330, Percent_Identity=37.5757575757576, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI87081858, Length=377, Percent_Identity=29.1777188328912, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=36.4406779661017, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=123, Percent_Identity=27.6422764227642, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49150; Mature: 49019

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQVIYIEDDALVRRASVQSLQLAGFDVGGFESAEAAEKAIVADTAGVIVSDIRLPG
CCCCEEEEEECCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCHHEECCCCCC
ASGLDVLAQCRERVPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRAL
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPVLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVADMPPETGAGDDADNDQTLKERIEQFERAVIAEALNQTGGAVA
CCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATADRLHVGKATLYEKMKRYGLSAKGETER
EEHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANRLQVIYIEDDALVRRASVQSLQLAGFDVGGFESAEAAEKAIVADTAGVIVSDIRLPG
CCCEEEEEECCHHHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHHCCHHEECCCCCC
ASGLDVLAQCRERVPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRAL
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYGRPAPVLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVADMPPETGAGDDADNDQTLKERIEQFERAVIAEALNQTGGAVA
CCHHHHHHHHHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATADRLHVGKATLYEKMKRYGLSAKGETER
EEHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]