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The map label for this gene is queF

Identifier: 116690757

GI number: 116690757

Start: 3034836

End: 3035666

Strand: Direct

Name: queF

Synonym: Bcen2424_2737

Alternate gene names: 116690757

Gene position: 3034836-3035666 (Clockwise)

Preceding gene: 116690756

Following gene: 116690762

Centisome position: 87.11

GC content: 66.55

Gene sequence:

>831_bases
ATGAATCCCGAACATTCCCCGCTCGGCAAGGCCACCGTCTACGCGGCGCAGTACGACGCGTCGCTGCTGTTCCCGATCCC
GCGCGCCGGCGCACGCGAGCAGCTCGGCATCACGTCGGCGCTGCCGTTCTTCGGCACCGACATCTGGAACGCGTACGAGC
TGTCGTGGCTCAATGCGCGCGGCAAGCCGCAGGTCGCGATCGCGACGTTCTACGTGCCGGCCGAATCGCCGAACATCGTC
GAATCGAAGTCGTTCAAGCTGTATCTCGGCTCGTTCGCGCAGTCGAAGTTCGACTCGGTCGATGCGGTGCGCGACGTGCT
GAAGCGCGACGTGTCGGCCGCGTGCGGCGCGAGCGTGTCGGTCCAGCTGGTGTCGCCGCACGATTTCGCCAAGCTGGAGA
TGGACGAGCTCGACGGGCTGTCGCTCGACCGGCTCGACCTCGACACCGACGTGTACGAACCCGATCCGTCGCTGCTGTCG
GCGGCCGACGGCGAGAACGAAGCGCCGGTCGAGGAGACGCTCGTGTCCGACCTGCTGCGCTCGAACTGCCCGGTCACGGG
CCAGCCCGACTGGGGCAGTGTGCAGATCCACTACGTCGGGCCGCAGATCGACCACGCGGGCCTGCTGCGCTACATCATCT
CGTTCCGCAATCACACGGGCTTTCACGAGCAGTGCGTCGAGCGAATCTTCCTCGACATCCTGCATGCGTGCAAACCGGTG
AAGCTCGCGGTGTACGCGCGCTACACGCGCCGCGGCGGGCTCGACATCAACCCGTTCCGCACGAATTACAACCAGCCGAT
GCCGGACAACGCGCGGACCGCGCGGCAGTGA

Upstream 100 bases:

>100_bases
CGCCGAATTCGAACGCTTCCTCGCGGCACTCGGCTACCCGTATGTCGAAGAGAGCGCCAACCCGGCTTACCGCCTCTTCC
TGTCGTAAAGGCTTCGTGCA

Downstream 100 bases:

>100_bases
AACTCGCCGGCGACCGTTCGCCCGCCCCTTCCGGGCGGCGGACGGCCCGGTTCATCCTTGCACCGGGCGCTCCGGGAGAA
TTTCCACCAGGCGCAGCAGG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQVAIATFYVPAESPNIV
ESKSFKLYLGSFAQSKFDSVDAVRDVLKRDVSAACGASVSVQLVSPHDFAKLEMDELDGLSLDRLDLDTDVYEPDPSLLS
AADGENEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPV
KLAVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ

Sequences:

>Translated_276_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQVAIATFYVPAESPNIV
ESKSFKLYLGSFAQSKFDSVDAVRDVLKRDVSAACGASVSVQLVSPHDFAKLEMDELDGLSLDRLDLDTDVYEPDPSLLS
AADGENEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPV
KLAVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
>Mature_276_residues
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNARGKPQVAIATFYVPAESPNIV
ESKSFKLYLGSFAQSKFDSVDAVRDVLKRDVSAACGASVSVQLVSPHDFAKLEMDELDGLSLDRLDLDTDVYEPDPSLLS
AADGENEAPVEETLVSDLLRSNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPV
KLAVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=52.5925925925926, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_BURCA (Q1BTM9)

Other databases:

- EMBL:   CP000378
- RefSeq:   YP_621999.1
- ProteinModelPortal:   Q1BTM9
- SMR:   Q1BTM9
- STRING:   Q1BTM9
- GeneID:   4091135
- GenomeReviews:   CP000378_GR
- KEGG:   bcn:Bcen_2125
- eggNOG:   COG2904
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   BCEN331271:BCEN_2125-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 30573; Mature: 30573

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHCCCHHCCCHHCCCCCCCEEEEEECCC
GKPQVAIATFYVPAESPNIVESKSFKLYLGSFAQSKFDSVDAVRDVLKRDVSAACGASVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VQLVSPHDFAKLEMDELDGLSLDRLDLDTDVYEPDPSLLSAADGENEAPVEETLVSDLLR
EEEECCCCCCEEEHHHCCCCCCCCEECCCCCCCCCHHHEECCCCCCCCCHHHHHHHHHHH
SNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPV
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCE
KLAVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
EEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPEHSPLGKATVYAAQYDASLLFPIPRAGAREQLGITSALPFFGTDIWNAYELSWLNAR
CCCCCCCCCCEEEEEEECCCEEEECCCCCCCHHHCCCHHCCCHHCCCCCCCEEEEEECCC
GKPQVAIATFYVPAESPNIVESKSFKLYLGSFAQSKFDSVDAVRDVLKRDVSAACGASVS
CCCCEEEEEEEEECCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VQLVSPHDFAKLEMDELDGLSLDRLDLDTDVYEPDPSLLSAADGENEAPVEETLVSDLLR
EEEECCCCCCEEEHHHCCCCCCCCEECCCCCCCCCHHHEECCCCCCCCCHHHHHHHHHHH
SNCPVTGQPDWGSVQIHYVGPQIDHAGLLRYIISFRNHTGFHEQCVERIFLDILHACKPV
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCE
KLAVYARYTRRGGLDINPFRTNYNQPMPDNARTARQ
EEEEEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA