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The map label for this gene is proP [H]

Identifier: 116690675

GI number: 116690675

Start: 2941838

End: 2943496

Strand: Direct

Name: proP [H]

Synonym: Bcen2424_2655

Alternate gene names: 116690675

Gene position: 2941838-2943496 (Clockwise)

Preceding gene: 116690674

Following gene: 116690678

Centisome position: 84.44

GC content: 65.58

Gene sequence:

>1659_bases
ATGGCAACGTTAGGCGGGCAAATTTCGCACTCGCCGATGACGGGCGAAGAGAAGAAGGTGATCTTCGCGTCGTCGCTCGG
CACCGTGTTCGAGTGGTACGACTTTTACCTGGCCGGCTCGCTCGCGGCCTACATCAGCAAGAGCTTCTTCTCCGGCGTCA
ATCCGACCGCCGCGTTCATCTTCACGCTGCTCGGCTTCGCCGCCGGCTTCGCGGTGCGGCCGTTCGGCGCGATCGTCTTC
GGCCGGCTCGGCGACCTCGTCGGCCGCAAGCACACGTTCCTCGTGACGATCGTGATCATGGGCGTCTCGACGTTCGTGGT
CGGCTTCCTCCCGGGCTACGCGTCGATCGGCATCGCCGCCCCCGTGATCTTCATCGCGATGCGGCTGTTGCAGGGCCTCG
CGCTCGGCGGCGAGTACGGCGGCGCGGCGACCTACGTCGCCGAGCATGCGCCGGCCAACCGGCGCGGCTTCTACACCGCC
TGGATCCAGACGACGGCCACGCTCGGCCTGTTCCTGTCGCTGCTCGTGATCCTGGGCGTGCGCACGTTCATCGGCGAGGA
AGCGTTCGGCAACTGGGGCTGGCGCGTGCCGTTCGTCGCATCGATCCTGCTGCTCGGGGTGTCGGTGTGGATCCGGCTGC
AACTGAACGAATCGCCGGTGTTCCTGCGCATCAAGGCGGAAGGCAAGACGTCGAAGGCGCCGCTGACCGAAGCGTTCGGC
CAGTGGAAGAACCTGAAGATCGTGATCCTCGCGCTGATCGGCCTGACGGCCGGCCAGGCGGTCGTGTGGTACACGGGCCA
GTTCTACGCGCTGTTCTTCCTCACGCAGACGCTGAAGGTCGACGGCGCCAGCGCGAACATCCTGATCGCGATCGCGCTCC
TGATCGGCACGCCGTTCTTCCTGTTCTTCGGCTCGCTGTCGGACAAGATCGGCCGCAAGCCGATCATCCTTGCCGGCTGC
CTGATCGCCGCGCTGACCTACTTCCCGCTGTTCAAGGCGCTCACGCACTACGCGAACCCGCAGCTCGAACTCGCGACGCA
GAAGGCGCCGATCACGGTCGTCGCCGATCCGGCTACCTGCTCGTTCCAGTTCAACCCGGTGGGCACGTCGAAGTTCACCA
CCTCGTGCGACATCGCGAAGAGCGCGCTCGCGAAGGCCGGCCTGAACTACGAGAACGTCGCGGCGCCGGCCGGCACGCTG
GCCGAAATCAAGGTCGGCGACACGGTGATCCAGACCTACGACGGCAAGGCGGCCGACGCGAAGGCGCAGGGCGCGGCGTT
CGACAAGACCCTCGCGTCGACGCTGAAGGGGGCCGGCTACCCGGCGAAGGCCGACCCGGCGCAACTCAACTGGCCGATGA
CGATCGTGATCCTGACGATCCTCGTGATCTTCGTGACGATGGTCTACGGCCCGATCGCCGCGATGCTGGTCGAGATGTTC
CCGACGCGAATCCGCTACACGTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGGCTTCCTGCCGGCAAC
TGCGTTCGCGATCGTCGCGGCGAAGGGCAACATCTACTCGGGGCTCTGGTATCCGATCGTGATCGCGCTGGCCACGTTCG
TGATCGGCCTGCTGTTCGTCAAGGAGACGAAGGACTCGGACATCTACGCGCAGGATTGA

Upstream 100 bases:

>100_bases
GCAAGTCAGTGCGCCGTAAGTTTCCGTGCCAATAATCGTCGACAGGCCCGCCCCTCCCAAGGCGGCCGCACATCTATATC
AAGGGACTTGGAGACGATTC

Downstream 100 bases:

>100_bases
TGTCGGGTCCGCGGCGGGGGAGGGCCGACGGAGGATCGCCTTCCGGCGCCCGGCTCCCGCCCGCGATGGAGGCCGGCACG
GACCGTCGGCAACTTTTTTC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATLGGQISHSPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGVRTFIGEEAFGNWGWRVPFVASILLLGVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLELATQKAPITVVADPATCSFQFNPVGTSKFTTSCDIAKSALAKAGLNYENVAAPAGTL
AEIKVGDTVIQTYDGKAADAKAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIFVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFVKETKDSDIYAQD

Sequences:

>Translated_552_residues
MATLGGQISHSPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGVRTFIGEEAFGNWGWRVPFVASILLLGVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLELATQKAPITVVADPATCSFQFNPVGTSKFTTSCDIAKSALAKAGLNYENVAAPAGTL
AEIKVGDTVIQTYDGKAADAKAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIFVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFVKETKDSDIYAQD
>Mature_551_residues
ATLGGQISHSPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVFG
RLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAW
IQTTATLGLFLSLLVILGVRTFIGEEAFGNWGWRVPFVASILLLGVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFGQ
WKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGCL
IAALTYFPLFKALTHYANPQLELATQKAPITVVADPATCSFQFNPVGTSKFTTSCDIAKSALAKAGLNYENVAAPAGTLA
EIKVGDTVIQTYDGKAADAKAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIFVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFVKETKDSDIYAQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.3658536585366, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788292, Length=338, Percent_Identity=33.4319526627219, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1788942, Length=339, Percent_Identity=33.9233038348083, Blast_Score=166, Evalue=4e-42,
Organism=Escherichia coli, GI1789941, Length=220, Percent_Identity=42.2727272727273, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=26.5486725663717, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=25.5520504731861, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 59045; Mature: 58914

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLGGQISHSPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTFIGEEAFGNWGWRVPFVASILLLGVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCCHHCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHYANPQLELATQKAPITVVADPATC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTTSCDIAKSALAKAGLNYENVAAPAGTLAEIKVGDTVIQTYDGKAADA
EEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCEEEEECCCCCCCC
KAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIFVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFV
CHHEEEEEECCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKDSDIYAQD
CCCCCCCCEECC
>Mature Secondary Structure 
ATLGGQISHSPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLVTIVIMGVSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGV
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTFIGEEAFGNWGWRVPFVASILLLGVSVWIRLQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCCHHCCCCCCCHHHHHHHHHHHHEEEEEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALIGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHYANPQLELATQKAPITVVADPATC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTTSCDIAKSALAKAGLNYENVAAPAGTLAEIKVGDTVIQTYDGKAADA
EEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCEEEEECCCCCCCC
KAQGAAFDKTLASTLKGAGYPAKADPAQLNWPMTIVILTILVIFVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGNIYSGLWYPIVIALATFVIGLLFV
CHHEEEEEECCCEEECCCCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHH
KETKDSDIYAQD
CCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]