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The map label for this gene is yddQ [H]

Identifier: 116690631

GI number: 116690631

Start: 2893631

End: 2894296

Strand: Reverse

Name: yddQ [H]

Synonym: Bcen2424_2611

Alternate gene names: 116690631

Gene position: 2894296-2893631 (Counterclockwise)

Preceding gene: 116690633

Following gene: 116690629

Centisome position: 83.08

GC content: 69.22

Gene sequence:

>666_bases
ATGTCGACCGCCGTTCCGCATTCCACTTCCCGTCCTGTTGCCCGCCGTGCACTGATCGTGATCGACGTGCAGAACGAATA
CGTGACGGGCAACCTGCCGATCGAATATCCGCCGCTCGACGTGTCGCTCGCGAACATCGGCCGCGCGATCGACGCCGCGC
ACGCGACCGGCGTGCCGGTGATCGTGGTTCAGCACGTCGCGCCGGCCGGCGCGCCGATCTTCGCGCCCGGTAGCGATGGC
GTCGCGCTGCACGCGGTGGTGGCCAGCCGGCCGTACGCGCACCTGATCGAGAAGGCGCAGGCGAGCTCGTTCGCCGGCAC
CGATCTCGCCGCGTGGCTCGACGCGCACGGAATCGACACGCTCGCGGTGGCCGGCTACATGACGCACAACTGCAATGCGT
CGACGGTCTATCACGCGGCGCATGCGGGGCTGAAGGTCGAATACCTGAACGACGCGACGGGCGCGCTGCCTTACGAGAAC
GAAGCGGGAGCGGTAAGCGCCGAAGAGATTCACCGCGCGTATGGCGTGGTGTTCCAGTCGAATTTCGCGGCGGTGATGTC
GACCGACACGTGGATCGCGGGGCTGGCCGGCGCGCCGATGCCGGCGCGCGATTCGGTGGCCGCGTCGAACCGGCGGGCCC
GCGCGCAGCGCGCGAAGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCACTATCGGCACAAGCCGCCCGGCTCGACAATGCATCGCATCGCACTGCTGGCCGCCCCGCTCCGGGGCGGGGCAGCGC
CTCCCGACCAGGAGCCCGCC

Downstream 100 bases:

>100_bases
CGCGGCACGGTGACGGCGGCGGGGCGCGCCATGCGCCGGGTGGTCAGTCGCGATCCTTCAGCATCGCCGGGCTGTAGCGC
AGCATGTCGAAGCAGCCGTC

Product: isochorismatase hydrolase

Products: Nicotinate; pyrazinoic acid; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MSTAVPHSTSRPVARRALIVIDVQNEYVTGNLPIEYPPLDVSLANIGRAIDAAHATGVPVIVVQHVAPAGAPIFAPGSDG
VALHAVVASRPYAHLIEKAQASSFAGTDLAAWLDAHGIDTLAVAGYMTHNCNASTVYHAAHAGLKVEYLNDATGALPYEN
EAGAVSAEEIHRAYGVVFQSNFAAVMSTDTWIAGLAGAPMPARDSVAASNRRARAQRAKAA

Sequences:

>Translated_221_residues
MSTAVPHSTSRPVARRALIVIDVQNEYVTGNLPIEYPPLDVSLANIGRAIDAAHATGVPVIVVQHVAPAGAPIFAPGSDG
VALHAVVASRPYAHLIEKAQASSFAGTDLAAWLDAHGIDTLAVAGYMTHNCNASTVYHAAHAGLKVEYLNDATGALPYEN
EAGAVSAEEIHRAYGVVFQSNFAAVMSTDTWIAGLAGAPMPARDSVAASNRRARAQRAKAA
>Mature_220_residues
STAVPHSTSRPVARRALIVIDVQNEYVTGNLPIEYPPLDVSLANIGRAIDAAHATGVPVIVVQHVAPAGAPIFAPGSDGV
ALHAVVASRPYAHLIEKAQASSFAGTDLAAWLDAHGIDTLAVAGYMTHNCNASTVYHAAHAGLKVEYLNDATGALPYENE
AGAVSAEEIHRAYGVVFQSNFAAVMSTDTWIAGLAGAPMPARDSVAASNRRARAQRAKAA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: 3.5.1.- [C]

Molecular weight: Translated: 22925; Mature: 22794

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAVPHSTSRPVARRALIVIDVQNEYVTGNLPIEYPPLDVSLANIGRAIDAAHATGVPV
CCCCCCCCCCCCCHHEEEEEEEECCCEEECCCCEECCCCCEEHHHHCHHHHHHHCCCCCE
IVVQHVAPAGAPIFAPGSDGVALHAVVASRPYAHLIEKAQASSFAGTDLAAWLDAHGIDT
EEEEECCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCH
LAVAGYMTHNCNASTVYHAAHAGLKVEYLNDATGALPYENEAGAVSAEEIHRAYGVVFQS
HHEEHEEECCCCCCEEEEHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHEEEEC
NFAAVMSTDTWIAGLAGAPMPARDSVAASNRRARAQRAKAA
CCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STAVPHSTSRPVARRALIVIDVQNEYVTGNLPIEYPPLDVSLANIGRAIDAAHATGVPV
CCCCCCCCCCCCHHEEEEEEEECCCEEECCCCEECCCCCEEHHHHCHHHHHHHCCCCCE
IVVQHVAPAGAPIFAPGSDGVALHAVVASRPYAHLIEKAQASSFAGTDLAAWLDAHGIDT
EEEEECCCCCCEEEECCCCCEEEEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHCCCCH
LAVAGYMTHNCNASTVYHAAHAGLKVEYLNDATGALPYENEAGAVSAEEIHRAYGVVFQS
HHEEHEEECCCCCCEEEEHHHCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHEEEEC
NFAAVMSTDTWIAGLAGAPMPARDSVAASNRRARAQRAKAA
CCEEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Nicotinamide; pyrazinamide; H2O [C]

Specific reaction: Nicotinamide + H2O = Nicotinate + NH3 pyrazinamide + H2O = pyrazinoic acid + NH3 [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]