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The map label for this gene is mexA [H]

Identifier: 116690629

GI number: 116690629

Start: 2891271

End: 2892545

Strand: Reverse

Name: mexA [H]

Synonym: Bcen2424_2609

Alternate gene names: 116690629

Gene position: 2892545-2891271 (Counterclockwise)

Preceding gene: 116690631

Following gene: 116690628

Centisome position: 83.03

GC content: 69.41

Gene sequence:

>1275_bases
ATGCGCGTCGAACGGGTTCCATACCGCTTAATCACTGTCGCGACGGCCGCCGTTTTCCTGGCCGCGTGCGGGAAAAAAGA
ATCGGCACCGCCGCCGCAAACGCCGGAAGTCGGCGTCGTCACCGTCCAGCCGCAAGCCGTACCGGTCTTCACCGACCTGC
CGGGCCGCACCAGTGCATTCCTCGTCGCGCAAGTGCGTGCGCGGGTCGACGGCATCGTGCTGCGCCGTGAATTCACCGAA
GGCACCGACGTCAAGGCCGGTCAGCGCCTCTACAAGATCGATCCGGCGCCGTACATCGCGGCGCTGAACAGCGCGAAGGC
GACGCTCGCGAAGGCGCAGGCGAACCTCGTCACGCAGAACGCGCTGGTCGCGCGCTACAAGGTGCTGGTGGCCGCGAACG
CGGTCAGCAAGCAGGACTACGACAACGCGGTCGCCACGCAAGGCCAGGCCGCGGCGGACGTCGCGGCCGGCAAGGCGGCG
GTCGATACCGCGCAGATCAACCTCGGCTATACGGACGTCGTGTCGCCGATCAGCGGCCGCGTCGGCATCTCGCAAGTGAC
GCCGGGCGCATACGTGCAGGCGAGCCAGGCGACGCTGATGTCGACCGTGCAGCAGCTCGATCCGGTGTACGTCGACCTCA
CGCAGTCGAGCCTCGAAGGGCTGAAGCTGCGTCAGGACGTGCAGAGCGGCCGTCTGAAGACGAGCGGCCCGGGCGCGGCG
AAGGTGTCGCTGATCCTGGAAGACGGCAAGACCTACTCGGAGCCGGGCAAGCTGCAGTTCTCGGACGTGACGGTCGACCA
GACCACCGGCTCGGTGACGATCCGCGCGGTGTTCCCGAACCCGGGCCGCGTGCTGCTGCCGGGGATGTTCGTGCGCGCAC
GGATCGAGGAAGGCGTGAACGAGAACGCCTTCCTGGTGCCGCAGATCGGCGTCACGCATGACCAGAAGGGCCAGGCGATC
GCGATGGTGGTGAACGCCAGCAACAAGGTCGAGCCGCGTCCGCTGAAGACCACCGGCATGCAAGGCCAGAACTGGATCGT
CGAAGGCGGTCTGCAGGCGGGCGATCACGTGATCGTGCAAGGCGTCGACAAGGTGCGCCCGGGCGCGACCGTGAAGACCG
TCGCCGCGCAGCTCGCCCCGGCGGCCGATGCCGCCTCGGGTGCCGCTGCCCCCGCCGCGCCGGCCGCTGCCGGGTCCGGC
GCCGCTGCCGCTTCCGGTGCCGCCGCATCGGGCGCCGCGCCGGCGAGTGCTGCCGCCGCGTCGAGCGCGCAATAA

Upstream 100 bases:

>100_bases
CCAGTAATTCAGGTGTTCGATCAGCGCCGCGAGGCGCATCCGTGTTGCGCTCGGGTCGGGCGCGGCACTTATTCCATTGG
TTATACACAGAGGTCGCTCC

Downstream 100 bases:

>100_bases
CAGGGAGCCTGTTTCATGGCAAAGTTTTTTATCGATCGCCCGATCTTCGCGTGGGTGATCGCCATCATCCTGATGCTGGC
CGGGGTTGCGGCGATCTTCA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTDLPGRTSAFLVAQVRARVDGIVLRREFTE
GTDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLVTQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAA
VDTAQINLGYTDVVSPISGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTSGPGAA
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVNENAFLVPQIGVTHDQKGQAI
AMVVNASNKVEPRPLKTTGMQGQNWIVEGGLQAGDHVIVQGVDKVRPGATVKTVAAQLAPAADAASGAAAPAAPAAAGSG
AAAASGAAASGAAPASAAAASSAQ

Sequences:

>Translated_424_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTDLPGRTSAFLVAQVRARVDGIVLRREFTE
GTDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLVTQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAA
VDTAQINLGYTDVVSPISGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTSGPGAA
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVNENAFLVPQIGVTHDQKGQAI
AMVVNASNKVEPRPLKTTGMQGQNWIVEGGLQAGDHVIVQGVDKVRPGATVKTVAAQLAPAADAASGAAAPAAPAAAGSG
AAAASGAAASGAAPASAAAASSAQ
>Mature_424_residues
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTDLPGRTSAFLVAQVRARVDGIVLRREFTE
GTDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLVTQNALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAA
VDTAQINLGYTDVVSPISGRVGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTSGPGAA
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVNENAFLVPQIGVTHDQKGQAI
AMVVNASNKVEPRPLKTTGMQGQNWIVEGGLQAGDHVIVQGVDKVRPGATVKTVAAQLAPAADAASGAAAPAAPAAAGSG
AAAASGAAASGAAPASAAAASSAQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=383, Percent_Identity=52.4804177545692, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1789665, Length=361, Percent_Identity=49.8614958448753, Blast_Score=335, Evalue=3e-93,
Organism=Escherichia coli, GI1789929, Length=368, Percent_Identity=47.2826086956522, Blast_Score=328, Evalue=3e-91,
Organism=Escherichia coli, GI87082036, Length=364, Percent_Identity=31.8681318681319, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43333; Mature: 43333

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTDLPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGTDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLVTQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAAVDTAQINLGYTDVVSPISGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTSGPGAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCEECCCCCCE
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCC
ENAFLVPQIGVTHDQKGQAIAMVVNASNKVEPRPLKTTGMQGQNWIVEGGLQAGDHVIVQ
CCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEE
GVDKVRPGATVKTVAAQLAPAADAASGAAAPAAPAAAGSGAAAASGAAASGAAPASAAAA
CHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
SSAQ
CCCC
>Mature Secondary Structure
MRVERVPYRLITVATAAVFLAACGKKESAPPPQTPEVGVVTVQPQAVPVFTDLPGRTSAF
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHH
LVAQVRARVDGIVLRREFTEGTDVKAGQRLYKIDPAPYIAALNSAKATLAKAQANLVTQN
HHHHHHHHHCEEEEEEECCCCCCCCCCCEEEEECCCCCEEECCCHHHHHHHHHHHHHHHH
ALVARYKVLVAANAVSKQDYDNAVATQGQAAADVAAGKAAVDTAQINLGYTDVVSPISGR
HHHHHEEEEEEECHHCCCCCCCHHHCCCCHHHHHHCCCCEEEEEEEECCHHHHHCCCCCC
VGISQVTPGAYVQASQATLMSTVQQLDPVYVDLTQSSLEGLKLRQDVQSGRLKTSGPGAA
CCCEECCCCCEEEHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHCCEECCCCCCE
KVSLILEDGKTYSEPGKLQFSDVTVDQTTGSVTIRAVFPNPGRVLLPGMFVRARIEEGVN
EEEEEEECCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCEECCCHHHHHHHHCCCC
ENAFLVPQIGVTHDQKGQAIAMVVNASNKVEPRPLKTTGMQGQNWIVEGGLQAGDHVIVQ
CCEEEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCEEEEE
GVDKVRPGATVKTVAAQLAPAADAASGAAAPAAPAAAGSGAAAASGAAASGAAPASAAAA
CHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
SSAQ
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]