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The map label for this gene is ogt [H]

Identifier: 116690580

GI number: 116690580

Start: 2835710

End: 2836177

Strand: Reverse

Name: ogt [H]

Synonym: Bcen2424_2560

Alternate gene names: 116690580

Gene position: 2836177-2835710 (Counterclockwise)

Preceding gene: 116690581

Following gene: 116690579

Centisome position: 81.41

GC content: 66.45

Gene sequence:

>468_bases
ATGTTCAACGCTGTCATCGACGCGCCGTTCGGCAAGGTCGGCATCCGCACGGATGCAGCGGTGGTGCGCGAGATCGTCTA
TCTGCCCGAATCGATGAAGTCGGTCGATCCGGATTCGGCGCTCGCGAAGCGCGCCGTGAAGCAGATCGAACGTTATTTCG
AGCGCGCGTCCGCACGCTTCGACCTGCCGCTCGCCGACGTCGGCAGTGCATTCCAGCACCGGGTGTGGGACGCGATCAGC
GATATCCCGCCCGGGACGGTGCTGACCTACGGCCAGGTCGCGAAGCGGATCGGCAGCGCGCCGCGCGCGGTCGGCCAGGC
GTGCGGCGCGAACTATTTCCCGCTCGTGATTCCGTGCCATCGCGTCGTCGCGGCGGGCGGTCTCGGCGGATTCGCGAACC
ACGACGACAACGGCTATTACCAGCAAGTGAAGCGCTGGCTGCTGACGCACGAGGGCGTGCCGTACTGA

Upstream 100 bases:

>100_bases
GAGCATGTCGAATGGGCGCTGCGAGCCGCGTGAAGGCGGCGTAAAGTGTCGTCGGCGCGTGCGCAGGGCCGCGCGCGCAT
CCCTCGGTCAGGAGAATTCA

Downstream 100 bases:

>100_bases
TGAGCGAACCGATGATTTCCCCCGCCGCCGACAACGACACCGACGCCGTCGCGGCATCGCCCGCGCTGCTCGCGAGCCGC
GCGTCGATCGACGTGTTCTG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MFNAVIDAPFGKVGIRTDAAVVREIVYLPESMKSVDPDSALAKRAVKQIERYFERASARFDLPLADVGSAFQHRVWDAIS
DIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQQVKRWLLTHEGVPY

Sequences:

>Translated_155_residues
MFNAVIDAPFGKVGIRTDAAVVREIVYLPESMKSVDPDSALAKRAVKQIERYFERASARFDLPLADVGSAFQHRVWDAIS
DIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQQVKRWLLTHEGVPY
>Mature_155_residues
MFNAVIDAPFGKVGIRTDAAVVREIVYLPESMKSVDPDSALAKRAVKQIERYFERASARFDLPLADVGSAFQHRVWDAIS
DIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCHRVVAAGGLGGFANHDDNGYYQQVKRWLLTHEGVPY

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=109, Percent_Identity=41.2844036697248, Blast_Score=76, Evalue=1e-15,
Organism=Escherichia coli, GI1788542, Length=117, Percent_Identity=35.042735042735, Blast_Score=71, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=39.7727272727273, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16903; Mature: 16903

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNAVIDAPFGKVGIRTDAAVVREIVYLPESMKSVDPDSALAKRAVKQIERYFERASARF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
DLPLADVGSAFQHRVWDAISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVAAGGLGGFANHDDNGYYQQVKRWLLTHEGVPY
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFNAVIDAPFGKVGIRTDAAVVREIVYLPESMKSVDPDSALAKRAVKQIERYFERASARF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCC
DLPLADVGSAFQHRVWDAISDIPPGTVLTYGQVAKRIGSAPRAVGQACGANYFPLVIPCH
CCCHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVAAGGLGGFANHDDNGYYQQVKRWLLTHEGVPY
HHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]