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The map label for this gene is kefA [H]

Identifier: 116690528

GI number: 116690528

Start: 2777420

End: 2778805

Strand: Reverse

Name: kefA [H]

Synonym: Bcen2424_2508

Alternate gene names: 116690528

Gene position: 2778805-2777420 (Counterclockwise)

Preceding gene: 116690529

Following gene: 116690527

Centisome position: 79.76

GC content: 69.05

Gene sequence:

>1386_bases
ATGGAGAATTTGCAGAGCCGCCTGCTGTCGCACCGGCTGGAATCGGTGATTCGCGATTTCCACCAGCCGGTGATGATCTG
GCAGGCGGCGATCCTCATCGGTGCGCTGTGTTTCGCGTGGGTGGCCGCGCGCTTCGTGCACGGCCGGATCGATGCGCGGC
GCCGGGCGGCCGGGCGTGCGCCCGGCGCGGGCGCGCAAAGCCTGAAACGTGCGTTGTTTCCGCTGTTCGGCAGCGTGTTC
GTCGGCGGCGCGCAACTCGCGTTCGACCCGTTCATGTCGACGTCGCTGTTGTCGCTCGCGCTCGTGCCGCTGTTCGGCAT
CGGGCTGATCTACGTGCTGTTCTTCTTCGCGCGGCGCGTGTTCGCGCGCGACGGCCACACGCACGCGTGGCTGTCGATCG
TCGAGAAGATCGTGTCGACCGTCGTGTGGGCCGCGATGGTGATGACCGTGCTCGGCATCCAGCGCGACGTGCTCGGCTGG
CTCGACAGCGTGCAGTTCCGGGTCGCGACCGCGCACCTCACGCTGCTGTCGGTGATCTCCGGCGCGCTGTGGGTGTGCGT
GACGCTGATGGTCGCGATGTGGCTCGGCTCGGTGCTCGAGGATCGCCTGTCGCGCGCGAGCACGCTCGACGCGAACCTGA
AGGTCGTACTGTCGCGGATCGGCCGCGCGCTGCTCGTGTTCGCGGCGATCCTGATCGGGCTGTCGCTGGTCGGCATCGAC
GTGACGGTGCTCGGCGTGTTCGGCGGCGCGGTGGGCGTCGGCCTCGGGTTCGGGCTGCAGAAGATCGCCAGCAACTACGT
GTCGGGCTTCATCATCCTGCTCGACCGCTCGCTGCGGCTCGGCGATGCGATCAGCGTCGGCGGGCTGCAGGGCGTCGTCA
CGCAGATCCGCACGCGCTACACGGTCGTGCGCGGCCTTGACGGCAACGAGACGCTGATCCCGAACGAGAAGCTGATCACC
GACGTCGTGCAGAACCAGTCGTCCTACCTGACGCGCGGCTACGCGAAAGTCGCGGTGCAGGTCGCGTACACGAGCGACGT
CGAGCAGGCACTCGCGTTGCTCGCCGACGCCGCGAAGGACGTGCCGCGCGTGCTGGCCGAGCCGGTGCCGACGCCGTACC
TCGTGGGGTTCGGCGCGGACGGCATCGATCTGGAGCTCGGCTTCTGGATCGAGGATTCGGCGAAAGGCACGTCGGGCGTG
CGCTCGAGCGTGAACCGCAACATCTGGCGGCTGTTCAGCGAGCACGGGATCTCGATTCCGTTTCCGCAGCGCGAAGTGCG
CGTGGTCGGGCTGCCGGACGGCTTTCCCGCGGCGGCGGGCGTGGCGGCCGGGCTCGATCCGGCGCTTGCCGATGGTGCGC
AGGACAGGCGCGCGCCGGCGGCGTAG

Upstream 100 bases:

>100_bases
CGACTACCTGTCGCTGTGGCCGCACCGTCACGCGACCGACGGCTTCTTCGCCGCGGTGCTCGAGCGCCGCGCCGCGCAGT
AACGACTGCCCGGACGGACG

Downstream 100 bases:

>100_bases
CGGCCACGGGCGGTCAATCGTACCGATTTCGCGGTCGAAACCGGATCACGGGCCTTTCCCAGTCCGGACATGGACCGCGC
GGATTGCGTCAGGGCAAGAA

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MENLQSRLLSHRLESVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRAPGAGAQSLKRALFPLFGSVF
VGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHTHAWLSIVEKIVSTVVWAAMVMTVLGIQRDVLGW
LDSVQFRVATAHLTLLSVISGALWVCVTLMVAMWLGSVLEDRLSRASTLDANLKVVLSRIGRALLVFAAILIGLSLVGID
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLIT
DVVQNQSSYLTRGYAKVAVQVAYTSDVEQALALLADAAKDVPRVLAEPVPTPYLVGFGADGIDLELGFWIEDSAKGTSGV
RSSVNRNIWRLFSEHGISIPFPQREVRVVGLPDGFPAAAGVAAGLDPALADGAQDRRAPAA

Sequences:

>Translated_461_residues
MENLQSRLLSHRLESVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRAPGAGAQSLKRALFPLFGSVF
VGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHTHAWLSIVEKIVSTVVWAAMVMTVLGIQRDVLGW
LDSVQFRVATAHLTLLSVISGALWVCVTLMVAMWLGSVLEDRLSRASTLDANLKVVLSRIGRALLVFAAILIGLSLVGID
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLIT
DVVQNQSSYLTRGYAKVAVQVAYTSDVEQALALLADAAKDVPRVLAEPVPTPYLVGFGADGIDLELGFWIEDSAKGTSGV
RSSVNRNIWRLFSEHGISIPFPQREVRVVGLPDGFPAAAGVAAGLDPALADGAQDRRAPAA
>Mature_461_residues
MENLQSRLLSHRLESVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRAPGAGAQSLKRALFPLFGSVF
VGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHTHAWLSIVEKIVSTVVWAAMVMTVLGIQRDVLGW
LDSVQFRVATAHLTLLSVISGALWVCVTLMVAMWLGSVLEDRLSRASTLDANLKVVLSRIGRALLVFAAILIGLSLVGID
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLIT
DVVQNQSSYLTRGYAKVAVQVAYTSDVEQALALLADAAKDVPRVLAEPVPTPYLVGFGADGIDLELGFWIEDSAKGTSGV
RSSVNRNIWRLFSEHGISIPFPQREVRVVGLPDGFPAAAGVAAGLDPALADGAQDRRAPAA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=252, Percent_Identity=34.9206349206349, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI2367355, Length=300, Percent_Identity=29, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1789291, Length=252, Percent_Identity=26.984126984127, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49393; Mature: 49393

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLQSRLLSHRLESVIRDFHQPVMIWQAAILIGALCFAWVAARFVHGRIDARRRAAGRA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PGAGAQSLKRALFPLFGSVFVGGAQLAFDPFMSTSLLSLALVPLFGIGLIYVLFFFARRV
CCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FARDGHTHAWLSIVEKIVSTVVWAAMVMTVLGIQRDVLGWLDSVQFRVATAHLTLLSVIS
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GALWVCVTLMVAMWLGSVLEDRLSRASTLDANLKVVLSRIGRALLVFAAILIGLSLVGID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTVLGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHH
TVVRGLDGNETLIPNEKLITDVVQNQSSYLTRGYAKVAVQVAYTSDVEQALALLADAAKD
HHHCCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHH
VPRVLAEPVPTPYLVGFGADGIDLELGFWIEDSAKGTSGVRSSVNRNIWRLFSEHGISIP
HHHHHHCCCCCCEEEEECCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
FPQREVRVVGLPDGFPAAAGVAAGLDPALADGAQDRRAPAA
CCCCCEEEEECCCCCCHHHHHHHCCCHHHHCCCCCCCCCCC
>Mature Secondary Structure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CCCCCEEEEECCCCCCHHHHHHHCCCHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]