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The map label for this gene is ascD [H]

Identifier: 116690510

GI number: 116690510

Start: 2760130

End: 2761161

Strand: Direct

Name: ascD [H]

Synonym: Bcen2424_2490

Alternate gene names: 116690510

Gene position: 2760130-2761161 (Clockwise)

Preceding gene: 116690508

Following gene: 116690511

Centisome position: 79.23

GC content: 66.86

Gene sequence:

>1032_bases
ATGGCATTCAACGTCACTCTCAAGCAAAGCGGCCGGCAATTCCAGGTCGAGTCGGACGAAACCGTGCTGGCGGCCGCGCT
GCGCCAGAACGTCCATCTGCCGTACGGCTGCAAGAACGGCGCGTGCGGCTCGTGCAAGGGCCAGATCGTATCGGGCCAGA
TCGAACAGGGCCCGCATGCCGCGTCGGCGCTGTCCAACGACGAACGCACGCGCGGCCTCGCGCTGCTGTGCTGCTCGAAG
GCGCAATGCGACCTCGAGATCGACGTGCGCGAGATCGCCGGCGTCGACGGCGTGCAGGTGAAGAAGCTGCCGTGCCGGAT
CGCCGCGCTCGAACGCAAGGCCGACGACGTGATGGTCGTGAAGCTGCAGTTGCCCGCGAACGAGCGCCTGCAGTACCTCG
CGGGCCAGTACGTCGAATTCATCCTGAAGGACGGCTCGCGCCGCAGCTACTCGATGGCGAACGCGCCGCACGAGGAAGGC
CCGATCGAACTGCACATCCGCCACATGCCGGGCGGCAAGTTCACCGACCACGTGTTCGGTGCGATGAAGGAGCGCGACAT
CCTGCGCTTCGAAGGCCCGCTCGGCACGTTCTTCCTGCGCGAGGATTCCGACAAGCCGATCGTGCTGCTCGCGTCGGGCA
CCGGCTTCGCGCCGATCAAGGCGATCATCGAGCACGTGAAGCATTCGGGCATCACCCGCCCGATGACGCTCTACTGGGGC
GCGCGCCGCAAGAAGGACATCTACCTCGCCGAACTCGCCGAGCAGTGGGCGCGCGAGATCCCGAACTTCAAGTACGTACC
GGTGCTCTCCGAGCCGGACGACGCCGACCAGTGGACGGGCCGCACCGGCTTCGTCCATCGCGCGGTGATCGAGGATCTGC
CCGATCTGTCGGGCCACCAGGTGTACGCGTGCGGCGCGCCGGTGATGGTCGAATCCGCGCAGCGCGACTTCACGCAGCAC
CACGCGCTGCCGGCCGACGAGTTCTACGCGGACTCGTTCACGAGCGCCGCCGATCTCGCGCATCCGGTCTGA

Upstream 100 bases:

>100_bases
GCGAAATCAGTAGCCGCCGTTGCGCGACGCGCAACGGCTTTGCATTGTAGCTGCCGTGCGCGACATGCACGGTCCCCCCA
TTTTGTTCTAGTTTGGTTCT

Downstream 100 bases:

>100_bases
GCACCGGGCGCGGCGCGCCCCCGCTGCGCGCCGCCGTGTCACATCCGTGGCATCGCGGCGGTTTACAGTGGGGCGCGGGA
TGCCTTATGCTTGCGCACAT

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MAFNVTLKQSGRQFQVESDETVLAAALRQNVHLPYGCKNGACGSCKGQIVSGQIEQGPHAASALSNDERTRGLALLCCSK
AQCDLEIDVREIAGVDGVQVKKLPCRIAALERKADDVMVVKLQLPANERLQYLAGQYVEFILKDGSRRSYSMANAPHEEG
PIELHIRHMPGGKFTDHVFGAMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHVKHSGITRPMTLYWG
ARRKKDIYLAELAEQWAREIPNFKYVPVLSEPDDADQWTGRTGFVHRAVIEDLPDLSGHQVYACGAPVMVESAQRDFTQH
HALPADEFYADSFTSAADLAHPV

Sequences:

>Translated_343_residues
MAFNVTLKQSGRQFQVESDETVLAAALRQNVHLPYGCKNGACGSCKGQIVSGQIEQGPHAASALSNDERTRGLALLCCSK
AQCDLEIDVREIAGVDGVQVKKLPCRIAALERKADDVMVVKLQLPANERLQYLAGQYVEFILKDGSRRSYSMANAPHEEG
PIELHIRHMPGGKFTDHVFGAMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHVKHSGITRPMTLYWG
ARRKKDIYLAELAEQWAREIPNFKYVPVLSEPDDADQWTGRTGFVHRAVIEDLPDLSGHQVYACGAPVMVESAQRDFTQH
HALPADEFYADSFTSAADLAHPV
>Mature_342_residues
AFNVTLKQSGRQFQVESDETVLAAALRQNVHLPYGCKNGACGSCKGQIVSGQIEQGPHAASALSNDERTRGLALLCCSKA
QCDLEIDVREIAGVDGVQVKKLPCRIAALERKADDVMVVKLQLPANERLQYLAGQYVEFILKDGSRRSYSMANAPHEEGP
IELHIRHMPGGKFTDHVFGAMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHVKHSGITRPMTLYWGA
RRKKDIYLAELAEQWAREIPNFKYVPVLSEPDDADQWTGRTGFVHRAVIEDLPDLSGHQVYACGAPVMVESAQRDFTQHH
ALPADEFYADSFTSAADLAHPV

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=235, Percent_Identity=29.7872340425532, Blast_Score=117, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6322146, Length=176, Percent_Identity=31.25, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6322699, Length=216, Percent_Identity=24.537037037037, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323552, Length=198, Percent_Identity=27.7777777777778, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323510, Length=165, Percent_Identity=26.0606060606061, Blast_Score=63, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 37910; Mature: 37778

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNVTLKQSGRQFQVESDETVLAAALRQNVHLPYGCKNGACGSCKGQIVSGQIEQGPHA
CEEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
ASALSNDERTRGLALLCCSKAQCDLEIDVREIAGVDGVQVKKLPCRIAALERKADDVMVV
HHHCCCCHHHCCEEEEEECCCCCCEEEEHHHHCCCCCCEEECCCHHHHHHHCCCCCEEEE
KLQLPANERLQYLAGQYVEFILKDGSRRSYSMANAPHEEGPIELHIRHMPGGKFTDHVFG
EEECCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEECCCCCHHHHHHH
AMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHVKHSGITRPMTLYWG
HHHCCCEEEEECCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEC
ARRKKDIYLAELAEQWAREIPNFKYVPVLSEPDDADQWTGRTGFVHRAVIEDLPDLSGHQ
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHCCCCCCCE
VYACGAPVMVESAQRDFTQHHALPADEFYADSFTSAADLAHPV
EEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCC
>Mature Secondary Structure 
AFNVTLKQSGRQFQVESDETVLAAALRQNVHLPYGCKNGACGSCKGQIVSGQIEQGPHA
EEEEEECCCCCEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCH
ASALSNDERTRGLALLCCSKAQCDLEIDVREIAGVDGVQVKKLPCRIAALERKADDVMVV
HHHCCCCHHHCCEEEEEECCCCCCEEEEHHHHCCCCCCEEECCCHHHHHHHCCCCCEEEE
KLQLPANERLQYLAGQYVEFILKDGSRRSYSMANAPHEEGPIELHIRHMPGGKFTDHVFG
EEECCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCEEEEEEECCCCCHHHHHHH
AMKERDILRFEGPLGTFFLREDSDKPIVLLASGTGFAPIKAIIEHVKHSGITRPMTLYWG
HHHCCCEEEEECCCEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEC
ARRKKDIYLAELAEQWAREIPNFKYVPVLSEPDDADQWTGRTGFVHRAVIEDLPDLSGHQ
CCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHCCCCCCHHHHHHHHHCCCCCCCE
VYACGAPVMVESAQRDFTQHHALPADEFYADSFTSAADLAHPV
EEECCCCCEEHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]