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The map label for this gene is sir1 [H]

Identifier: 116690495

GI number: 116690495

Start: 2745542

End: 2747221

Strand: Reverse

Name: sir1 [H]

Synonym: Bcen2424_2475

Alternate gene names: 116690495

Gene position: 2747221-2745542 (Counterclockwise)

Preceding gene: 116690498

Following gene: 116690494

Centisome position: 78.85

GC content: 65.71

Gene sequence:

>1680_bases
ATGTATCAGTACGACCAATACGACCAGACGATCGTCGACGAGCGAGTCGCGCAGTACCGCGATCAGGTGCGCCGCCGGCT
GTCGGGCGAGTTGAGCGAAGACGAATTCCGTCCGCTGCGTCTGCAGAACGGCCTGTACATGCAGCGCCACGCGTACATGC
ACCGCATCGCGATTCCGTACGGCAACCTGCGCAGCGACCAGCTCCGGATGCTGGCGCGCATCGCCCGCGAACACGACCGC
GGCTACGGCCATTTCTCGACCCGCTCGAACATCCAGTTCAACTGGATCGAGCTGGAAGACACACCCGAAATCCTCGCGAA
GCTCGCGTCGGTGCAGATGCATGGCATCCAGACGTCGGGCAACTGCATCCGCAACATCACGGCCGACCAGTTCGCCGGCG
TCGCGCAGGACGAGGAGATCGATCCGCGTCCGTGGGCCGAGATCCTGCGCCAGTGGTCGACGTTCCACCCCGAATTCGCG
TGGCTGCCGCGCAAGTTCAAGATCGCGGTGTCGGGCTCGAAGCGTGACCGCGCGGCCGTGCAGATCCACGACCTCGGCGT
GTACCTGAAGAAGAACGCGCAGGGCGAGGTGGTCGCGAGCATCCTCGCGGGCGGCGGCCTCGGCCGTACGCCGATCGTCG
GCGCGGTGATCAAGGAAGACCTGCCGTGGCAGCACCTGCTCACGTACTGCGAAGCCGTGCTGCGCGTGTACAACCGTTAC
GGCCGCCGCGACAACCTGTACAAGGCGCGCATCAAGATCCTCGTGAAGGCGCTGTCGCCGGCGAAGTTCGCGCAGCAGGT
CGAGGAAGAATGGCAGCACCTGAAGGACGGCCCGTCGACGCTCACGCAGGCCGAAATCGACCGCGTGTCGCAGTACTTCC
AGCCGCCCGTCTACGAGAAGCTGGCCGACACCGACGCGTCGTTCGAACAGCACCTGCTCGAGAACAAGGCGTTCGCGCGC
TGGGTCGAGCGTAACGTCGCGCCGCACAAGGTGCCGGGCTATGCGGCCGTCACGCTGTCGCTGAAGGACCACCGCGTGGC
CCCGGGCGACGCGACCGACGCGCAGATGGATCTGGTCGCCGACTGGGCGGACGCGTATTCGTTGGGCGAGCTGCGCGTGT
CGCACGAGCAGAACCTGATTCTCGCGAACGTGAAGAAGCGCGACCTGTTCGCGCTGTGGGAAAAGGCGAAGGCGGCCGGT
TTCGCGACGCCGAACGTCGGCCTGCTGACCGACATCATCGCGTGCCCGGGCGGCGACTTCTGCTCGCTCGCGAACGCGAA
GTCGATCCCGATCGCGCTGGCGATCCAGCAGCGTTTCGACGATCTGGACTACGTGTACGACCTCGGCGACCTGTCGCTGA
ACATTTCCGGTTGCATGAACTCGTGCGGTCACCACCACGTCGGCAACATCGGCATCTTGGGCGTCGACAAGGACGGCGCC
GAGTGGTACCAGGTGTCGCTCGGCGGCGAGCAGGGCACGGGCCGCAACGGCGCGCGTCTCGGCCGCGTGATCGGGCCGTC
GTTCTCGGCGGAAGAAGTGCCCGACGTGATCGCGAAGCTGATCGATACGTTCGTCGAGGCGCGCATCGACGGCGAGCGCT
TCATCGACACGTATGATCGCATCGGCATCGCGCCGTTCAAGGAGCGCGTGTATGCGGCGCGCCAGGCAGTGAACGCGTAA

Upstream 100 bases:

>100_bases
CCAGCCGGCAGCGGTTGGCGGCGGTGACGACGGAACGGAGATTCGGGCGCGACGTGGCTAGGACGTCGCGCAGTCACCAC
GAAAACCCTGGGGTCCCCGA

Downstream 100 bases:

>100_bases
TCAACCGGGTAGAGGGAATTTGCAGATGGCTTCGATTATCAAGAACCGCGCAGTGATCGACGACGCATGGCAGGTCGTAC
GCGCGGCGGAAGACGGTACG

Product: nitrite/sulfite reductase hemoprotein beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MYQYDQYDQTIVDERVAQYRDQVRRRLSGELSEDEFRPLRLQNGLYMQRHAYMHRIAIPYGNLRSDQLRMLARIAREHDR
GYGHFSTRSNIQFNWIELEDTPEILAKLASVQMHGIQTSGNCIRNITADQFAGVAQDEEIDPRPWAEILRQWSTFHPEFA
WLPRKFKIAVSGSKRDRAAVQIHDLGVYLKKNAQGEVVASILAGGGLGRTPIVGAVIKEDLPWQHLLTYCEAVLRVYNRY
GRRDNLYKARIKILVKALSPAKFAQQVEEEWQHLKDGPSTLTQAEIDRVSQYFQPPVYEKLADTDASFEQHLLENKAFAR
WVERNVAPHKVPGYAAVTLSLKDHRVAPGDATDAQMDLVADWADAYSLGELRVSHEQNLILANVKKRDLFALWEKAKAAG
FATPNVGLLTDIIACPGGDFCSLANAKSIPIALAIQQRFDDLDYVYDLGDLSLNISGCMNSCGHHHVGNIGILGVDKDGA
EWYQVSLGGEQGTGRNGARLGRVIGPSFSAEEVPDVIAKLIDTFVEARIDGERFIDTYDRIGIAPFKERVYAARQAVNA

Sequences:

>Translated_559_residues
MYQYDQYDQTIVDERVAQYRDQVRRRLSGELSEDEFRPLRLQNGLYMQRHAYMHRIAIPYGNLRSDQLRMLARIAREHDR
GYGHFSTRSNIQFNWIELEDTPEILAKLASVQMHGIQTSGNCIRNITADQFAGVAQDEEIDPRPWAEILRQWSTFHPEFA
WLPRKFKIAVSGSKRDRAAVQIHDLGVYLKKNAQGEVVASILAGGGLGRTPIVGAVIKEDLPWQHLLTYCEAVLRVYNRY
GRRDNLYKARIKILVKALSPAKFAQQVEEEWQHLKDGPSTLTQAEIDRVSQYFQPPVYEKLADTDASFEQHLLENKAFAR
WVERNVAPHKVPGYAAVTLSLKDHRVAPGDATDAQMDLVADWADAYSLGELRVSHEQNLILANVKKRDLFALWEKAKAAG
FATPNVGLLTDIIACPGGDFCSLANAKSIPIALAIQQRFDDLDYVYDLGDLSLNISGCMNSCGHHHVGNIGILGVDKDGA
EWYQVSLGGEQGTGRNGARLGRVIGPSFSAEEVPDVIAKLIDTFVEARIDGERFIDTYDRIGIAPFKERVYAARQAVNA
>Mature_559_residues
MYQYDQYDQTIVDERVAQYRDQVRRRLSGELSEDEFRPLRLQNGLYMQRHAYMHRIAIPYGNLRSDQLRMLARIAREHDR
GYGHFSTRSNIQFNWIELEDTPEILAKLASVQMHGIQTSGNCIRNITADQFAGVAQDEEIDPRPWAEILRQWSTFHPEFA
WLPRKFKIAVSGSKRDRAAVQIHDLGVYLKKNAQGEVVASILAGGGLGRTPIVGAVIKEDLPWQHLLTYCEAVLRVYNRY
GRRDNLYKARIKILVKALSPAKFAQQVEEEWQHLKDGPSTLTQAEIDRVSQYFQPPVYEKLADTDASFEQHLLENKAFAR
WVERNVAPHKVPGYAAVTLSLKDHRVAPGDATDAQMDLVADWADAYSLGELRVSHEQNLILANVKKRDLFALWEKAKAAG
FATPNVGLLTDIIACPGGDFCSLANAKSIPIALAIQQRFDDLDYVYDLGDLSLNISGCMNSCGHHHVGNIGILGVDKDGA
EWYQVSLGGEQGTGRNGARLGRVIGPSFSAEEVPDVIAKLIDTFVEARIDGERFIDTYDRIGIAPFKERVYAARQAVNA

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

None

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62906; Mature: 62906

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQYDQYDQTIVDERVAQYRDQVRRRLSGELSEDEFRPLRLQNGLYMQRHAYMHRIAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHEECCC
GNLRSDQLRMLARIAREHDRGYGHFSTRSNIQFNWIELEDTPEILAKLASVQMHGIQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCC
NCIRNITADQFAGVAQDEEIDPRPWAEILRQWSTFHPEFAWLPRKFKIAVSGSKRDRAAV
CHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCEEEEEECCCCCCCCEE
QIHDLGVYLKKNAQGEVVASILAGGGLGRTPIVGAVIKEDLPWQHLLTYCEAVLRVYNRY
EEEECCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GRRDNLYKARIKILVKALSPAKFAQQVEEEWQHLKDGPSTLTQAEIDRVSQYFQPPVYEK
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHH
LADTDASFEQHLLENKAFARWVERNVAPHKVPGYAAVTLSLKDHRVAPGDATDAQMDLVA
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
DWADAYSLGELRVSHEQNLILANVKKRDLFALWEKAKAAGFATPNVGLLTDIIACPGGDF
HHHHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
CSLANAKSIPIALAIQQRFDDLDYVYDLGDLSLNISGCMNSCGHHHVGNIGILGVDKDGA
CCCCCCCCCCEEEEEHHHCCCHHHHEECCCCEEEHHHHHHCCCCCCCCCEEEEEECCCCC
EWYQVSLGGEQGTGRNGARLGRVIGPSFSAEEVPDVIAKLIDTFVEARIDGERFIDTYDR
CEEEEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IGIAPFKERVYAARQAVNA
CCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYQYDQYDQTIVDERVAQYRDQVRRRLSGELSEDEFRPLRLQNGLYMQRHAYMHRIAIPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHEECCC
GNLRSDQLRMLARIAREHDRGYGHFSTRSNIQFNWIELEDTPEILAKLASVQMHGIQTSG
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHCCCCCC
NCIRNITADQFAGVAQDEEIDPRPWAEILRQWSTFHPEFAWLPRKFKIAVSGSKRDRAAV
CHHHCCCHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCHHCCCCEEEEEECCCCCCCCEE
QIHDLGVYLKKNAQGEVVASILAGGGLGRTPIVGAVIKEDLPWQHLLTYCEAVLRVYNRY
EEEECCEEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GRRDNLYKARIKILVKALSPAKFAQQVEEEWQHLKDGPSTLTQAEIDRVSQYFQPPVYEK
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHH
LADTDASFEQHLLENKAFARWVERNVAPHKVPGYAAVTLSLKDHRVAPGDATDAQMDLVA
HHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHH
DWADAYSLGELRVSHEQNLILANVKKRDLFALWEKAKAAGFATPNVGLLTDIIACPGGDF
HHHHHHHHHHEEECCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCC
CSLANAKSIPIALAIQQRFDDLDYVYDLGDLSLNISGCMNSCGHHHVGNIGILGVDKDGA
CCCCCCCCCCEEEEEHHHCCCHHHHEECCCCEEEHHHHHHCCCCCCCCCEEEEEECCCCC
EWYQVSLGGEQGTGRNGARLGRVIGPSFSAEEVPDVIAKLIDTFVEARIDGERFIDTYDR
CEEEEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
IGIAPFKERVYAARQAVNA
CCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA