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The map label for this gene is cysD [H]

Identifier: 116690492

GI number: 116690492

Start: 2743192

End: 2744154

Strand: Reverse

Name: cysD [H]

Synonym: Bcen2424_2472

Alternate gene names: 116690492

Gene position: 2744154-2743192 (Counterclockwise)

Preceding gene: 116690493

Following gene: 116690491

Centisome position: 78.77

GC content: 67.08

Gene sequence:

>963_bases
ATGAGCACGACGCTCGAGCAATCCGCCTTTGCCCCGCCCACCGGCGCCGACAGCCGCATGGGCCACCTCGACTGGCTCGA
AGCCGAGTCGATCCACATCCTGCGCGAACTGGTTGCGGAATGCAGCAAGCCGGCGCTGTTGTTCTCGGGCGGCAAGGATT
CGGTCGTCGTGCTGCATCTGGCGCTGAAGGCGTTCGGCCTCGGCGCGAACCGCAAGACGACGCTGCCGTTCCCGCTCGTG
CACATCGACACGGGCCACAACTACGAGGAAGTGATCGACTTCCGCGACCGCCGTGCGCAAGAGCTCGGCGCCGAGCTGGT
GGTCGGTCACGTCGAGGATTCGATCAAGCGCGGCACGGTCGTGCTGCGCCGCGAGACCGATTCGCGCAACGCCGCGCAGG
CCGTCACGCTGCTCGAGACGATCGAACAGCACGGCTACACGGCGCTGATCGGCGGCGCGCGCCGCGACGAAGAGAAGGCC
CGCGCGAAGGAGCGGATCTTCTCGTTCCGCGACGAATTCGGCCAGTGGGACCCGAAGGCGCAGCGCCCGGAACTGTGGAG
CCTGTACAACGCCCGCCTGCACAAGGGCGAGCATCTGCGCGTGTTCCCGATCTCGAACTGGACCGAGCTCGACGTGTGGC
AGTACATCGCGCGCGAGAACCTCGAACTGCCGTCGATCTATTACGCGCACCAGCGCGAGATCGTGCGCCGCAACGGGCTG
CTCGTGCCGGTCACGCCGCTCACGCCGATGCGTGACGGCGAGACGAGCGAGCTGGCGCAGGTGCGCTTCCGCACGGTCGG
CGACATCAGCTGCACGTGCCCGGTCGAGAGCGACGCGGACGACGTCGAGAAGATCATCGCCGAGACGGCGGTGACCGAGA
TCACCGAGCGCGGCGCGACCCGGATGGACGACCAGGCCTCCGAAGCCGCGATGGAACAGCGCAAGAAGCAAGGTTATTTC
TGA

Upstream 100 bases:

>100_bases
GCCGAAGCCGGCGCCGCGCACTGAACGCCCGATAAGAAACTGAATATTGCGCCGCCTGCGACAGCGGGCGGCACGAACCC
AGAAGAGAAGGACTGAAATC

Downstream 100 bases:

>100_bases
AGCACACGAGGACATTCACATCATGAGCATCATCGAGAACACCGAAGACCTCGGCGTGTTGCGTTTCATCACCGCGGGCA
GCGTCGACGACGGCAAGAGC

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSTTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRKTTLPFPLV
HIDTGHNYEEVIDFRDRRAQELGAELVVGHVEDSIKRGTVVLRRETDSRNAAQAVTLLETIEQHGYTALIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
LVPVTPLTPMRDGETSELAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGYF

Sequences:

>Translated_320_residues
MSTTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRKTTLPFPLV
HIDTGHNYEEVIDFRDRRAQELGAELVVGHVEDSIKRGTVVLRRETDSRNAAQAVTLLETIEQHGYTALIGGARRDEEKA
RAKERIFSFRDEFGQWDPKAQRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
LVPVTPLTPMRDGETSELAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGYF
>Mature_319_residues
STTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHLALKAFGLGANRKTTLPFPLVH
IDTGHNYEEVIDFRDRRAQELGAELVVGHVEDSIKRGTVVLRRETDSRNAAQAVTLLETIEQHGYTALIGGARRDEEKAR
AKERIFSFRDEFGQWDPKAQRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGLL
VPVTPLTPMRDGETSELAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGATRMDDQASEAAMEQRKKQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=304, Percent_Identity=46.3815789473684, Blast_Score=268, Evalue=2e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 36139; Mature: 36008

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHH
ALKAFGLGANRKTTLPFPLVHIDTGHNYEEVIDFRDRRAQELGAELVVGHVEDSIKRGTV
HHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHEEHHHHHHHCCCEE
VLRRETDSRNAAQAVTLLETIEQHGYTALIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPMRDGETSELAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGAT
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQASEAAMEQRKKQGYF
CCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STTLEQSAFAPPTGADSRMGHLDWLEAESIHILRELVAECSKPALLFSGGKDSVVVLHL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEHHHH
ALKAFGLGANRKTTLPFPLVHIDTGHNYEEVIDFRDRRAQELGAELVVGHVEDSIKRGTV
HHHHHCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCHHHEEHHHHHHHCCCEE
VLRRETDSRNAAQAVTLLETIEQHGYTALIGGARRDEEKARAKERIFSFRDEFGQWDPKA
EEEECCCCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCC
QRPELWSLYNARLHKGEHLRVFPISNWTELDVWQYIARENLELPSIYYAHQREIVRRNGL
CCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCC
LVPVTPLTPMRDGETSELAQVRFRTVGDISCTCPVESDADDVEKIIAETAVTEITERGAT
EEEECCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCC
RMDDQASEAAMEQRKKQGYF
CCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA