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The map label for this gene is cysN [H]

Identifier: 116690491

GI number: 116690491

Start: 2741853

End: 2743169

Strand: Reverse

Name: cysN [H]

Synonym: Bcen2424_2471

Alternate gene names: 116690491

Gene position: 2743169-2741853 (Counterclockwise)

Preceding gene: 116690492

Following gene: 116690490

Centisome position: 78.74

GC content: 67.35

Gene sequence:

>1317_bases
ATGAGCATCATCGAGAACACCGAAGACCTCGGCGTGTTGCGTTTCATCACCGCGGGCAGCGTCGACGACGGCAAGAGCAC
GCTGATCGGCCGCCTGCTGTACGACAGCAAGGCCGTGCTGTCCGACCAGCTGTCCGCGCTGTCGCGCGCGAAGAACAAGC
GCACGGTCGGCGACGAGCTGGATCTCGCGCTGCTGACCGACGGCCTCGAAGCCGAGCGCGAGCAGGGCATCACGATCGAC
GTCGCGTACCGCTACTTCGCGACCGCGAAGCGCAAGTTCATCATCGCCGACACGCCGGGCCACGAGCAGTACACGCGCAA
CATGGTGACGGGCGCGTCGACCGCGCACGCGGCGATCGTGCTGATCGACGCGACGCGCATCACGGTCGAGAACGGCGTCG
TGCAACTGCTGCCGCAGACCAAGCGCCACAGCGCGATCGTCAAGCTGCTCGGCCTGCAGCACGTGATCGTCGCGATCAAC
AAAATGGACCTCGTCGACTACAGCGAGGCGCGTTTCAACGAGATCCGCGACGCGTACGTCGCGCTCGCGAAGCAGCTCGG
CCTGACCGACGTGCGTTTCGTGCCGGTGTCGGCGCTGAAGGGCGACAACATCGTCGGCGCGAGCGAGCGGATGCCGTGGT
ACGCGGGCGAGCCGCTCCTCGACGTGCTCGAGTCGCTGCCGGTCGAGACGCAGGCGCATGACGCGCTGCGCTTCCCGGTG
CAGTGGGTCGCGCGCCAGGACGGCAGCTCGGCTGACGATTTCCGCGGTTACATGGGCCGCATCGAGTCGGGCGAAGTGAA
GGTCGGCGACGCGATCGTCGTGCTGCCGTCGAACCGCACCGCGACGGTTGCCGAGATCGTCGCGCCGGTGCCGGGCGGCA
CCGCGGCAGTTCCGCACGCGTTCGCGGGCCAGACGGTGACGATCCGTCTCGAAGAGGACGTCGACGTGTCGCGCGGCGAC
ATGTTCGTCACGACCGCCGAGCCGGTCGAGCCGGCCAAGAAGCTCGAGGCCGACCTGTGCTGGTTCGACGAGGCGCCGCT
GTCGCCGCAGCGCAAGTACCTGCTGAAGCAGACCACCAGCACGGTGTTCGCGAAGATCGGCGCGGTCAAGCAGGTGCTCG
ACGTGCATACGCTGTCGCACGCGACCGACCGTCAGGAACTGAAGATGAACGACATCGGCCGCGTGGCGCTGACGCTGCAA
AAGCCGATCGTCTGCGACACGTACGATGCGCACCCCGGTACGGGCGCGTTCGTGCTGATCGACGAGGCGACGCATCACAC
GGTCGCCGCGGGCATGATCCGCGCGTTCTCGGCGTAA

Upstream 100 bases:

>100_bases
GAGCGCGGCGCGACCCGGATGGACGACCAGGCCTCCGAAGCCGCGATGGAACAGCGCAAGAAGCAAGGTTATTTCTGAAG
CACACGAGGACATTCACATC

Downstream 100 bases:

>100_bases
TCGTCAATCTGCGCGGCGGCGAACCGGCCGCCGCGGCAGGACCTTAGCGAAGCGACAAACATGGGCAAGGTGTATCTGAT
CGGAGCAGGACCGGGCGCGG

Product: sulfate adenylyltransferase, large subunit

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDELDLALLTDGLEAEREQGITID
VAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIVLIDATRITVENGVVQLLPQTKRHSAIVKLLGLQHVIVAIN
KMDLVDYSEARFNEIRDAYVALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVETQAHDALRFPV
QWVARQDGSSADDFRGYMGRIESGEVKVGDAIVVLPSNRTATVAEIVAPVPGGTAAVPHAFAGQTVTIRLEEDVDVSRGD
MFVTTAEPVEPAKKLEADLCWFDEAPLSPQRKYLLKQTTSTVFAKIGAVKQVLDVHTLSHATDRQELKMNDIGRVALTLQ
KPIVCDTYDAHPGTGAFVLIDEATHHTVAAGMIRAFSA

Sequences:

>Translated_438_residues
MSIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDELDLALLTDGLEAEREQGITID
VAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIVLIDATRITVENGVVQLLPQTKRHSAIVKLLGLQHVIVAIN
KMDLVDYSEARFNEIRDAYVALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVETQAHDALRFPV
QWVARQDGSSADDFRGYMGRIESGEVKVGDAIVVLPSNRTATVAEIVAPVPGGTAAVPHAFAGQTVTIRLEEDVDVSRGD
MFVTTAEPVEPAKKLEADLCWFDEAPLSPQRKYLLKQTTSTVFAKIGAVKQVLDVHTLSHATDRQELKMNDIGRVALTLQ
KPIVCDTYDAHPGTGAFVLIDEATHHTVAAGMIRAFSA
>Mature_437_residues
SIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDELDLALLTDGLEAEREQGITIDV
AYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIVLIDATRITVENGVVQLLPQTKRHSAIVKLLGLQHVIVAINK
MDLVDYSEARFNEIRDAYVALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVETQAHDALRFPVQ
WVARQDGSSADDFRGYMGRIESGEVKVGDAIVVLPSNRTATVAEIVAPVPGGTAAVPHAFAGQTVTIRLEEDVDVSRGDM
FVTTAEPVEPAKKLEADLCWFDEAPLSPQRKYLLKQTTSTVFAKIGAVKQVLDVHTLSHATDRQELKMNDIGRVALTLQK
PIVCDTYDAHPGTGAFVLIDEATHHTVAAGMIRAFSA

Specific function: May be the GTPase, regulating ATP sulfurylase activity [H]

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI223555963, Length=434, Percent_Identity=29.9539170506912, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI5729864, Length=434, Percent_Identity=29.9539170506912, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI4503475, Length=445, Percent_Identity=27.8651685393258, Blast_Score=161, Evalue=9e-40,
Organism=Homo sapiens, GI194018522, Length=426, Percent_Identity=28.6384976525822, Blast_Score=159, Evalue=7e-39,
Organism=Homo sapiens, GI194018520, Length=426, Percent_Identity=28.6384976525822, Blast_Score=158, Evalue=8e-39,
Organism=Homo sapiens, GI4503471, Length=446, Percent_Identity=28.47533632287, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI194097354, Length=426, Percent_Identity=28.6384976525822, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI46094014, Length=426, Percent_Identity=28.169014084507, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI34147630, Length=153, Percent_Identity=33.9869281045752, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1789108, Length=426, Percent_Identity=50.2347417840376, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI2367247, Length=364, Percent_Identity=25, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1790412, Length=148, Percent_Identity=31.7567567567568, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1789737, Length=148, Percent_Identity=31.0810810810811, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17552884, Length=445, Percent_Identity=29.438202247191, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17569207, Length=445, Percent_Identity=29.438202247191, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI115532067, Length=345, Percent_Identity=33.9130434782609, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI115532065, Length=343, Percent_Identity=33.8192419825073, Blast_Score=172, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI32566303, Length=445, Percent_Identity=27.6404494382022, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=439, Percent_Identity=27.5626423690205, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI32566301, Length=144, Percent_Identity=32.6388888888889, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=271, Percent_Identity=28.0442804428044, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556456, Length=342, Percent_Identity=26.6081871345029, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322937, Length=364, Percent_Identity=30.2197802197802, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325337, Length=448, Percent_Identity=27.2321428571429, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=448, Percent_Identity=27.2321428571429, Blast_Score=152, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=441, Percent_Identity=28.7981859410431, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324761, Length=271, Percent_Identity=29.1512915129151, Blast_Score=89, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652838, Length=451, Percent_Identity=29.7117516629712, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17137572, Length=451, Percent_Identity=29.7117516629712, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45553807, Length=451, Percent_Identity=28.8248337028825, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45553816, Length=451, Percent_Identity=28.8248337028825, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24651721, Length=451, Percent_Identity=28.8248337028825, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17864154, Length=451, Percent_Identity=28.8248337028825, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45550900, Length=438, Percent_Identity=29.4520547945205, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137380, Length=438, Percent_Identity=27.1689497716895, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI281363316, Length=265, Percent_Identity=30.9433962264151, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17864358, Length=265, Percent_Identity=30.9433962264151, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI19921738, Length=159, Percent_Identity=32.7044025157233, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 47584; Mature: 47453

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEL
CCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
DLALLTDGLEAEREQGITIDVAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIV
CEEEEECCCHHHHHCCCEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEE
LIDATRITVENGVVQLLPQTKRHSAIVKLLGLQHVIVAINKMDLVDYSEARFNEIRDAYV
EEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHHHHHH
ALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVETQAHDALRFPV
HHHHHCCCCEEEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCH
QWVARQDGSSADDFRGYMGRIESGEVKVGDAIVVLPSNRTATVAEIVAPVPGGTAAVPHA
HHHHHCCCCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCEEHHHHHCCCCCCCCCCCCC
FAGQTVTIRLEEDVDVSRGDMFVTTAEPVEPAKKLEADLCWFDEAPLSPQRKYLLKQTTS
CCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHCCCEEECCCCCCCHHHHHHHHHHHH
TVFAKIGAVKQVLDVHTLSHATDRQELKMNDIGRVALTLQKPIVCDTYDAHPGTGAFVLI
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCEEEEE
DEATHHTVAAGMIRAFSA
ECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SIIENTEDLGVLRFITAGSVDDGKSTLIGRLLYDSKAVLSDQLSALSRAKNKRTVGDEL
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCC
DLALLTDGLEAEREQGITIDVAYRYFATAKRKFIIADTPGHEQYTRNMVTGASTAHAAIV
CEEEEECCCHHHHHCCCEEEEEHHHHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCEEEE
LIDATRITVENGVVQLLPQTKRHSAIVKLLGLQHVIVAINKMDLVDYSEARFNEIRDAYV
EEEEEEEEECCCEEEECCCCHHHHHHHHHHHHHEEEHEEECCCCCCCCHHHHHHHHHHHH
ALAKQLGLTDVRFVPVSALKGDNIVGASERMPWYAGEPLLDVLESLPVETQAHDALRFPV
HHHHHCCCCEEEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHCCH
QWVARQDGSSADDFRGYMGRIESGEVKVGDAIVVLPSNRTATVAEIVAPVPGGTAAVPHA
HHHHHCCCCCHHHHHHHHCCCCCCCEEECCEEEEECCCCCEEHHHHHCCCCCCCCCCCCC
FAGQTVTIRLEEDVDVSRGDMFVTTAEPVEPAKKLEADLCWFDEAPLSPQRKYLLKQTTS
CCCCEEEEEEECCCCCCCCCEEEEECCCCCHHHHHCCCEEECCCCCCCHHHHHHHHHHHH
TVFAKIGAVKQVLDVHTLSHATDRQELKMNDIGRVALTLQKPIVCDTYDAHPGTGAFVLI
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEEEEEECCCEEEECCCCCCCCCEEEEE
DEATHHTVAAGMIRAFSA
ECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA