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The map label for this gene is btuF [H]

Identifier: 116690470

GI number: 116690470

Start: 2722750

End: 2723667

Strand: Direct

Name: btuF [H]

Synonym: Bcen2424_2450

Alternate gene names: 116690470

Gene position: 2722750-2723667 (Clockwise)

Preceding gene: 116690469

Following gene: 116690476

Centisome position: 78.15

GC content: 71.35

Gene sequence:

>918_bases
ATGAGCGCCCCCATGTTCCGCCGGCTCGCGCCGGCCGCGCTGCTGGCCGCGCTCGCTCACGCACCGCTCGTCCACGCCGA
CGTCACGACCCGCGACGACGCCGGCAACACGGTCACGTTACCCGCGCCTGCCCAGCGCGTAATCAGCCTCGCGCCGCACG
CGACCGAGCTCGTCTATGCGGCCGGCGGCGGCGCGAAGCTCGTCGGCACCGTCACGTACAGCGACTATCCGGCCGCCGCG
CAAGCGGTGCCACGCGTCGGCGACAACAAGGCGCTCGATCTCGAACGCATCGCCGCGCTGAAGCCCGACCTGATCGTCGT
GTGGCGGCACGGCAACGCCGAGCGGCAGACCGACGCGCTGCGCGCGCTGCACATTCCGCTGTTCTTCAGCGAACCGAAGC
ATCTCGACGACGTGTCGTCGTCGTTGCGCCGGCTCGGCACGCTGCTCGGCACGCAACCGACTGCCGATGCCGCCGCGGCC
GCCTTTACCCGAGACATCGCGACACTGCGCGCACGCTACGCGGCACGCCCGCCCGTCACGATGTTCTTCCAGGTGTGGGA
CCGCCCGCTGACGACGCTCAACGGCACGCATCTGATCAACGAAGTGTTCGAACTCTGCGGCGGCCGCAACGTATTCGCGT
CGCTCAAGCCGCTCGCGCCGACCGTGACCGACGAGGCCGTGCTCGCGGCGAATCCGGAAGCGATCGTGACGACGAGTGCC
GGCGCGACCCGTTCGAACGAACCGCTGCCGAGCCTCGCGCGCTGGCGTGCGTGGCCCGCGCTGACGGCCGTGGCGCGCAA
CAACCTGTTCGCGATCGACGGCGATCTGCTGACGCGGCCATCGCCGCGGATCGCGCAGGGTGCGGCCGCGCTGTGCGAGG
ATCTGGATGCCGCGCGTGCGCGGCGGCCGGTGCGCTGA

Upstream 100 bases:

>100_bases
CGCCGTCGAGCGTACGCATCGGCCTGCCGGCGAGCGAAGCCGAATGGCAGCGCCTCGGCGACGCGCTCGCGCATTGCGTG
CCGCTGCTGAAGGCATCCGC

Downstream 100 bases:

>100_bases
TTTCCCGCGCAGCGGCTCGCGCGTTCATCGCGCATCGGTGCCGGATGCGTCCGGCGGCCGAGCGCAGATCGATCCGTGTG
CCGCGTCGTTCGCTTGCAGA

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSAPMFRRLAPAALLAALAHAPLVHADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSDYPAAA
QAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFFSEPKHLDDVSSSLRRLGTLLGTQPTADAAAA
AFTRDIATLRARYAARPPVTMFFQVWDRPLTTLNGTHLINEVFELCGGRNVFASLKPLAPTVTDEAVLAANPEAIVTTSA
GATRSNEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPVR

Sequences:

>Translated_305_residues
MSAPMFRRLAPAALLAALAHAPLVHADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSDYPAAA
QAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFFSEPKHLDDVSSSLRRLGTLLGTQPTADAAAA
AFTRDIATLRARYAARPPVTMFFQVWDRPLTTLNGTHLINEVFELCGGRNVFASLKPLAPTVTDEAVLAANPEAIVTTSA
GATRSNEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPVR
>Mature_304_residues
SAPMFRRLAPAALLAALAHAPLVHADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYAAGGGAKLVGTVTYSDYPAAAQ
AVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDALRALHIPLFFSEPKHLDDVSSSLRRLGTLLGTQPTADAAAAA
FTRDIATLRARYAARPPVTMFFQVWDRPLTTLNGTHLINEVFELCGGRNVFASLKPLAPTVTDEAVLAANPEAIVTTSAG
ATRSNEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARARRPVR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=36.1111111111111, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 32498; Mature: 32367

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPMFRRLAPAALLAALAHAPLVHADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYA
CCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEECCCCHHHHHHCCCCHHEEEEE
AGGGAKLVGTVTYSDYPAAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDAL
CCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
RALHIPLFFSEPKHLDDVSSSLRRLGTLLGTQPTADAAAAAFTRDIATLRARYAARPPVT
HHEECEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
MFFQVWDRPLTTLNGTHLINEVFELCGGRNVFASLKPLAPTVTDEAVLAANPEAIVTTSA
HHHHHHCCCHHHCCHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCEEEECCCCEEEEECC
GATRSNEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRPVR
CCCCC
>Mature Secondary Structure 
SAPMFRRLAPAALLAALAHAPLVHADVTTRDDAGNTVTLPAPAQRVISLAPHATELVYA
CCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCEEECCCCHHHHHHCCCCHHEEEEE
AGGGAKLVGTVTYSDYPAAAQAVPRVGDNKALDLERIAALKPDLIVVWRHGNAERQTDAL
CCCCCEEEEEEEECCCCHHHHHHCCCCCCCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
RALHIPLFFSEPKHLDDVSSSLRRLGTLLGTQPTADAAAAAFTRDIATLRARYAARPPVT
HHEECEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
MFFQVWDRPLTTLNGTHLINEVFELCGGRNVFASLKPLAPTVTDEAVLAANPEAIVTTSA
HHHHHHCCCHHHCCHHHHHHHHHHHHCCCCEEHHCCCCCCCCCCCEEEECCCCEEEEECC
GATRSNEPLPSLARWRAWPALTAVARNNLFAIDGDLLTRPSPRIAQGAAALCEDLDAARA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHH
RRPVR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA