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The map label for this gene is purD [H]

Identifier: 116690316

GI number: 116690316

Start: 2550717

End: 2551997

Strand: Direct

Name: purD [H]

Synonym: Bcen2424_2295

Alternate gene names: 116690316

Gene position: 2550717-2551997 (Clockwise)

Preceding gene: 116690315

Following gene: 116690317

Centisome position: 73.21

GC content: 67.14

Gene sequence:

>1281_bases
ATGAAACTACTCGTCGTCGGTTCCGGCGGCCGCGAACATGCGCTGGCGTGGAAGCTCGCGCAATCGCCGCGCGTCCAGAT
GGTCTACGTCGCGCCCGGCAATGGCGGCACGGCGCAGGACGAGCGTCTGAAGAACGTCGACATCACGGCGCTCGACGAAC
TCGCCGATTTCGCGGAGCGCGAAGGCGTTGCGTTCACGCTCGTCGGGCCGGAAGCGCCGCTCGCGGCCGGCATCGTCAAC
CTGTTCCGCGCACGCGGCCTGAAGGTATTCGGCCCGACCCGCGAAGCCGCGCAGCTCGAAAGCTCGAAGGATTTCGCGAA
GGCGTTCATGAAGCGCCACAACATTCCGACCGCCGACTACGAAACCTTCTCCGATGCGGCCGCCGCCCACGCGTACATCG
ACGCGAAGGGCGCGCCGATCGTCGTGAAGGCCGACGGCCTCGCGGCTGGCAAGGGCGTCGTCGTCGCGATGACGCTCGAA
GAGGCGCATGCGGCCGTCGACATGATGCTGTCGGGCAACAAGCTCGGCGATGCCGGCGCGCGCGTCGTGATCGAGGAATT
CCTCGATGGCGAGGAAGCCAGCTTCATCGTGATGGTCGACGGCAAGCACGCGCTGGCGCTGGCCTCCAGCCAGGACCACA
AGCGCCTGCTCGACGAGGATCGCGGCCCGAACACCGGCGGCATGGGCGCGTATTCGCCCGCGCCGATCGTCACGCCGCAG
ATGCATGCACGCGTGATGCGCGAGATCATCATGCCGACCGTGCGCGGGATGGAGAAGGACGGCATCCGCTTCACCGGCTT
CCTGTACGCGGGCCTGATGATCGACAAGGACGGCAATCCGCGCACGCTCGAGTTCAACTGCCGGATGGGCGACCCGGAAA
CGCAACCGATCATGGCGCGCCTGAAGAGCGATTTCTCGAAGGTCGTCGAGCAGGCGATCGCGGGCACGCTCGACACGGTC
GAGCTCGACTGGGATCGCCGCACCGCGCTGGGCGTCGTGCTCGCCGCGCACGGCTACCCGGACGCGCCGCGCAAGGGCGA
CCGTATCAACGGAATCCCGGCCGAGACCGAACAGGCCGTGACGTTCCATGCGGGCACGACGCTCGCCGACGGCGACAAGC
TCGTGACGTCGGGCGGCCGCGTGCTGTGCGTGGTCGGCCTCGCCGATTCGGTGCGCGAAGCGCAGCAGCATGCGTACGAC
ACGATCAACCAGATCAATTTCGAAGGCATGCAGTACCGCCGCGACATCGGCTTTCGCGCGCTCAACCGCAAGAGCACGTG
A

Upstream 100 bases:

>100_bases
CAGCCGCCGCGCGCAGCGTTGCGACGGCGGCCCGACCGATTTCGCGGCCGGCGCGCCTGAGCGTGCCGGCCGCCCTGTTT
TCTAGTCTGCGGGGAATCCC

Downstream 100 bases:

>100_bases
CGTCGTGATCGCCGCCCCGCGCCCGCACGGCGGCGGCCCCTCTGCCGGGGTTCGATAGAATGCCCGGCAGATGCTTTTTT
TACGGCCCTCGCGTCAGGCG

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAEREGVAFTLVGPEAPLAAGIVN
LFRARGLKVFGPTREAAQLESSKDFAKAFMKRHNIPTADYETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHAAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKDGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAVTFHAGTTLADGDKLVTSGGRVLCVVGLADSVREAQQHAYD
TINQINFEGMQYRRDIGFRALNRKST

Sequences:

>Translated_426_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAEREGVAFTLVGPEAPLAAGIVN
LFRARGLKVFGPTREAAQLESSKDFAKAFMKRHNIPTADYETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHAAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKDGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAVTFHAGTTLADGDKLVTSGGRVLCVVGLADSVREAQQHAYD
TINQINFEGMQYRRDIGFRALNRKST
>Mature_426_residues
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAEREGVAFTLVGPEAPLAAGIVN
LFRARGLKVFGPTREAAQLESSKDFAKAFMKRHNIPTADYETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLE
EAHAAVDMMLSGNKLGDAGARVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKDGNPRTLEFNCRMGDPETQPIMARLKSDFSKVVEQAIAGTLDTV
ELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAVTFHAGTTLADGDKLVTSGGRVLCVVGLADSVREAQQHAYD
TINQINFEGMQYRRDIGFRALNRKST

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=425, Percent_Identity=50.1176470588235, Blast_Score=401, Evalue=1e-112,
Organism=Homo sapiens, GI4503915, Length=425, Percent_Identity=50.1176470588235, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869995, Length=425, Percent_Identity=50.1176470588235, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI209869993, Length=425, Percent_Identity=50.1176470588235, Blast_Score=400, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=429, Percent_Identity=60.8391608391608, Blast_Score=526, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=45.0236966824645, Blast_Score=346, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6321203, Length=449, Percent_Identity=42.3162583518931, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI62471619, Length=431, Percent_Identity=45.4756380510441, Blast_Score=352, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24582400, Length=434, Percent_Identity=45.3917050691244, Blast_Score=351, Evalue=6e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 45820; Mature: 45820

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHH
EGVAFTLVGPEAPLAAGIVNLFRARGLKVFGPTREAAQLESSKDFAKAFMKRHNIPTADY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLEEAHAAVDMMLSGNKLGDAGA
CHHHHHHHHEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHEECCCCCCCCHH
RVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
HHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKDGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLADGDKLVTSGGRVLCVVGLADSVREAQQHAYDTINQINFEGMQYRRDIGFRA
EEECCCEECCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHH
LNRKST
HCCCCC
>Mature Secondary Structure
MKLLVVGSGGREHALAWKLAQSPRVQMVYVAPGNGGTAQDERLKNVDITALDELADFAER
CEEEEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCCHHHHCCCCHHHHHHHHHHHHH
EGVAFTLVGPEAPLAAGIVNLFRARGLKVFGPTREAAQLESSKDFAKAFMKRHNIPTADY
CCCEEEEECCCCCHHHHHHHHHHHCCCEEECCCHHHHHHHCCHHHHHHHHHHCCCCCCCC
ETFSDAAAAHAYIDAKGAPIVVKADGLAAGKGVVVAMTLEEAHAAVDMMLSGNKLGDAGA
CHHHHHHHHEEEEECCCCEEEEEECCCCCCCCEEEEEEHHHHHHHHHHEECCCCCCCCHH
RVVIEEFLDGEEASFIVMVDGKHALALASSQDHKRLLDEDRGPNTGGMGAYSPAPIVTPQ
HHHHHHHCCCCCCEEEEEECCCEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
MHARVMREIIMPTVRGMEKDGIRFTGFLYAGLMIDKDGNPRTLEFNCRMGDPETQPIMAR
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEEECCCCCCEEEEEEECCCCCCHHHHHH
LKSDFSKVVEQAIAGTLDTVELDWDRRTALGVVLAAHGYPDAPRKGDRINGIPAETEQAV
HHHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCEE
TFHAGTTLADGDKLVTSGGRVLCVVGLADSVREAQQHAYDTINQINFEGMQYRRDIGFRA
EEECCCEECCCCEEEECCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCHHH
LNRKST
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]