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The map label for this gene is pnp [H]

Identifier: 116690274

GI number: 116690274

Start: 2507019

End: 2509166

Strand: Reverse

Name: pnp [H]

Synonym: Bcen2424_2253

Alternate gene names: 116690274

Gene position: 2509166-2507019 (Counterclockwise)

Preceding gene: 116690275

Following gene: 116690273

Centisome position: 72.02

GC content: 65.04

Gene sequence:

>2148_bases
ATGTCCATGTTCAACAAGGTCGTGAAGGAATTCCAGTGGGGCCAGCACAAGGTGCGCCTCGAAACCGGTGAAGTCGCCCG
CCAGGCGAGCGGCGCCGTGATCGTCGACGTCGAAGACACCGTCGTGCTGGCAACCGTCGTCGGCGCGAAGTCGGCGAAGC
CGGGTCAGGATTTCTTCCCGTTGACCGTCGACTACCTCGAGAAGACCTACTCGGCCGGCAAGATCCCGGGCGGCTTCTTC
CGCCGCGAAGGCCGTCCGTCGGAGCACGAGACGCTGACGTCGCGCCTGATCGACCGTCCGCTGCGCCCGCTGTTCCCGGA
AGGCTTCTACAACGAAGTCCAGGTCGTGATCCACGTGCTGTCCGTGAACCCGGAAATCCCGGCCGACATCCCCGCGCTGA
TCGGCGCATCGGCCGCGCTCGCCGTGTCGGGCCTGCCGTTCAACGGCCCGGTCGGTGCCGCACGCGTGGCATACATCGAC
AACGCATACGTGCTGAACCCGACGCGTGACCAGCTCAAGGCATCGAGCCTCGACCTCGTCGTCGCGGGTACGGAGCGCGC
CGTGCTGATGGTCGAGTCGGAAGCCGACCAACTGTCGGAAGAAGTGATGCTGGGCGCCGTGGTGTTCGGCCACGAGCAGA
TGCAAATCGCGATCGACGCGATCCACGAACTGGTGCGCGACGGCGGCAAGCCCGAGTGGGACTGGCAGCCGGCGCCGAAG
AACGAGGCGCTGATCGCACGCGTGACCGAGCTGGCGCAGAACGATCTGCTCGCCGCTTACCAGCTGCGCGACAAGCAGGC
GCGTTCGGCGAAGCTGAAGGAAGTCTATGCGGCAACCTCGGCGAAGCTCGAGGAAGACGCGCTGGCGGCCGGTACGGTCG
CGGCCGACAAGGCCACGGTCGGCAACGTGCTGTTCGACATCGAAGCGAAGATCGTCCGTTCGCAGATCCTGAACGGCGAG
CCGCGCATCGACGGCCGTGACACGCGCACCGTGCGTCCGATCGAAATCCGCACCGGCGTGCTGCCGCGTACCCACGGCTC
GGCGCTGTTCACGCGCGGCGAGACGCAGGCGCTGGTCGTCGCGACGCTCGGCACGAAGGGTGACGAGCAGATCATCGACG
CGCTCGAAGGCGAATACCGCGAGCGCTTCATGCTCCACTACAACATGCCCCCGTTCGCGACCGGCGAAACGGGCCGCGTC
GGCTCGCCGAAGCGCCGCGAAATCGGCCACGGCCGGCTGGCCAAGCGCGCGCTGGTCAAGTGCCTGCCGAGCGCCGACGA
ATTCGGCTACTCGATCCGCGTCGTGTCGGAAATCACCGAGTCGAACGGTTCGTCGTCGATGGCATCGGTGTGCGGCGGCT
GCCTCGCGCTGATGGACGCCGGCGTGCCGATGAAGGCGCACGTCGCGGGTATCGCGATGGGCCTGATCCTCGAAGGCAAC
AAGTTCGCGGTGCTGACCGACATCCTCGGCGACGAAGATCACCTCGGCGACATGGACTTCAAGGTGGCCGGCACGGAGCA
AGGCGTGACGGCGCTGCAGATGGACATCAAGATCCAGGGCATCACGAAGGAAATCATGCAGGTCGCGCTCGCGCAGGCGA
AGGAAGGCCGCCTGCACATCCTCGGCAAGATGACGTCGGCGGTGTCGGGTGCGAACACGCAACTGTCGGAATTCGCGCCG
CGCATGATCACGGTCAAGATCAACCCGGAAAAGATCCGCGACGTGATCGGCAAGGGCGGTTCGGTGATCCGCGCGCTGAC
GGAAGAAACGGGCACGACCATCGACATCTCGGATGACGGCGTCGTGACGATCGCCAGCACGAGCAGCGAAGGCATGGCCG
AAGCGAAGAAGCGCATCGAGCAAATCACGGCCGAGATCGAAGTCGGTCAGGTGTATGAAGGCACGGTGCTCAAGCTGCTC
GATTTCGGCGCGATCGTGAATCTGCTGCCGGGCAAGGATGGCCTGCTGCACATCTCGGAAATCGTCAACGAGCGCGTGAA
GGACATCAACGACTACCTGAAGGAAGGTCAGCAGGTGAAGGTCAAGGTGATCCAGACGGACGAGAAGGGTCGCGTGCGTC
TGTCGGCCAAGGCGCTGCTGAATGAAGCAGCGGCGGCAGCGCAGTCGGATACGCCGCCGCAGCAGTAA

Upstream 100 bases:

>100_bases
GGCATAAAAAACACACCTTGCTCCGGGTGATTCGGTCGGGACGCCGCCGCGCGGGATGGCCGGCGCGGCGAATGAAATGC
ATGAATACAAAGGAGCAACC

Downstream 100 bases:

>100_bases
GCGGACAGGCGGATCAACGGCCGGCAGTGCGAAAGCGTGCCGGCCGTTTTTTCTGTAGCATCGTTGTGCGGCGCAGCAGC
GCCGGCGAGGCGCCCGCGCC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MSMFNKVVKEFQWGQHKVRLETGEVARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYLEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYID
NAYVLNPTRDQLKASSLDLVVAGTERAVLMVESEADQLSEEVMLGAVVFGHEQMQIAIDAIHELVRDGGKPEWDWQPAPK
NEALIARVTELAQNDLLAAYQLRDKQARSAKLKEVYAATSAKLEEDALAAGTVAADKATVGNVLFDIEAKIVRSQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVKCLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITKEIMQVALAQAKEGRLHILGKMTSAVSGANTQLSEFAP
RMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ

Sequences:

>Translated_715_residues
MSMFNKVVKEFQWGQHKVRLETGEVARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYLEKTYSAGKIPGGFF
RREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYID
NAYVLNPTRDQLKASSLDLVVAGTERAVLMVESEADQLSEEVMLGAVVFGHEQMQIAIDAIHELVRDGGKPEWDWQPAPK
NEALIARVTELAQNDLLAAYQLRDKQARSAKLKEVYAATSAKLEEDALAAGTVAADKATVGNVLFDIEAKIVRSQILNGE
PRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRV
GSPKRREIGHGRLAKRALVKCLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITKEIMQVALAQAKEGRLHILGKMTSAVSGANTQLSEFAP
RMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGTVLKLL
DFGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ
>Mature_714_residues
SMFNKVVKEFQWGQHKVRLETGEVARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFPLTVDYLEKTYSAGKIPGGFFR
REGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIDN
AYVLNPTRDQLKASSLDLVVAGTERAVLMVESEADQLSEEVMLGAVVFGHEQMQIAIDAIHELVRDGGKPEWDWQPAPKN
EALIARVTELAQNDLLAAYQLRDKQARSAKLKEVYAATSAKLEEDALAAGTVAADKATVGNVLFDIEAKIVRSQILNGEP
RIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVVATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVG
SPKRREIGHGRLAKRALVKCLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGNK
FAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITKEIMQVALAQAKEGRLHILGKMTSAVSGANTQLSEFAPR
MITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDGVVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGTVLKLLD
FGAIVNLLPGKDGLLHISEIVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=724, Percent_Identity=37.9834254143646, Blast_Score=449, Evalue=1e-126,
Organism=Escherichia coli, GI145693187, Length=697, Percent_Identity=65.1362984218078, Blast_Score=931, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=32.3033707865169, Blast_Score=324, Evalue=1e-88,
Organism=Drosophila melanogaster, GI281362905, Length=715, Percent_Identity=35.6643356643357, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651641, Length=715, Percent_Identity=35.6643356643357, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24651643, Length=715, Percent_Identity=35.6643356643357, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=35.5855855855856, Blast_Score=377, Evalue=1e-104,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 77023; Mature: 76892

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMFNKVVKEFQWGQHKVRLETGEVARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYLEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIDNAYVLNPTRDQLKASSLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCEEEECCCHHHHCCCCCEEE
VAGTERAVLMVESEADQLSEEVMLGAVVFGHEQMQIAIDAIHELVRDGGKPEWDWQPAPK
EECCCEEEEEEECCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALIARVTELAQNDLLAAYQLRDKQARSAKLKEVYAATSAKLEEDALAAGTVAADKATV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHH
GNVLFDIEAKIVRSQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVK
EEECCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITKEIMQVALAQAKEGRLHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEE
LGKMTSAVSGANTQLSEFAPRMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
EEHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SMFNKVVKEFQWGQHKVRLETGEVARQASGAVIVDVEDTVVLATVVGAKSAKPGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCCCCC
LTVDYLEKTYSAGKIPGGFFRREGRPSEHETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CCHHHHHHHHCCCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHEEEE
SVNPEIPADIPALIGASAALAVSGLPFNGPVGAARVAYIDNAYVLNPTRDQLKASSLDLV
ECCCCCCCCCHHHHCCCHHHEEECCCCCCCCCCEEEEEECCEEEECCCHHHHCCCCCEEE
VAGTERAVLMVESEADQLSEEVMLGAVVFGHEQMQIAIDAIHELVRDGGKPEWDWQPAPK
EECCCEEEEEEECCHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALIARVTELAQNDLLAAYQLRDKQARSAKLKEVYAATSAKLEEDALAAGTVAADKATV
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHH
GNVLFDIEAKIVRSQILNGEPRIDGRDTRTVRPIEIRTGVLPRTHGSALFTRGETQALVV
CCEEEEHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCEEEECCCCCEEEE
ATLGTKGDEQIIDALEGEYRERFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALVK
EEECCCCHHHHHHHHCCHHHEEEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHH
CLPSADEFGYSIRVVSEITESNGSSSMASVCGGCLALMDAGVPMKAHVAGIAMGLILEGN
HCCCCHHCCEEEEEEEHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHEEEEEEEECC
KFAVLTDILGDEDHLGDMDFKVAGTEQGVTALQMDIKIQGITKEIMQVALAQAKEGRLHI
CEEEEEECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEE
LGKMTSAVSGANTQLSEFAPRMITVKINPEKIRDVIGKGGSVIRALTEETGTTIDISDDG
EEHHHHHHCCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCC
VVTIASTSSEGMAEAKKRIEQITAEIEVGQVYEGTVLKLLDFGAIVNLLPGKDGLLHISE
EEEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHH
IVNERVKDINDYLKEGQQVKVKVIQTDEKGRVRLSAKALLNEAAAAAQSDTPPQQ
HHHHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA