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The map label for this gene is gcvA [H]

Identifier: 116690197

GI number: 116690197

Start: 2424052

End: 2424993

Strand: Direct

Name: gcvA [H]

Synonym: Bcen2424_2176

Alternate gene names: 116690197

Gene position: 2424052-2424993 (Clockwise)

Preceding gene: 116690194

Following gene: 116690198

Centisome position: 69.58

GC content: 72.29

Gene sequence:

>942_bases
ATGTCAACGCCACTCGTCCGTCTCCCGTCGCTCGACCTCGTCCGCGGTTTCGTCGCCGTCGGCCGCCGCATGAGCATCAC
GCTCGCCGCGCAGGATCTGTGCGTCACGCAGTCCGCGGTCAGCCGCCAGGTCCATGCGCTCGAAGCGCATCTCGGCGTGA
CGTTGCTGCAGCGCGGCTACCGGAAGATCGCGTTCACGCCGGAAGGCGAACGGCTGTTCCGCGTCGCCGATGCGGCGCTG
CTCGGCCTGCAGGACACCGTCGCGTCGCTCGCCGCGGCGCGCGAGCGCCAGCCCGTGACGATCACCGCCAGCATCGGCGT
GACCGCGCTGTGGCTGCTGCCGCGGCTCGGGCGGCTGCAGGCGCGGCTGCCCGGGATCGACCTGCGCGTCGCCGCGAACG
ACAAGGTACTCGATTTGCGCGCCGAAGGCATCGACCTCGGCATCCGCTACTGCCCGCGCGAGCGCGCGCCGGCCGGCGCG
CTGCGCCTGTTCGACGAGATCGTCGTGCCGGTCGCGCACCCGGCGCTCGCCGCGCGACCGGTCACGAACGCGGCCGCGCT
CGCCGATCACGTGCTGCTCGAATTCGACGGGCCACCGCAACCGCAGCTACAGTGGCACGCGCACCTGCACGCGGCCGGGC
TCGGCGACGCGCGCCCGAAAGGCGTGCTGCGCTTCAACCAGTACGACCAGGTGATCCAGGCCGCGATCGCGGGCCAGGGC
ATCGCGCTCGGCCGGCTCGCGCTCGTCGCGCCGATGCTCGCCGACGGCCGGCTCGCGGTGCTCGGGCCGCACACGCAGAC
GCTGTCGCAGACGTACGGCTACTGGCTGTTCCAGCACGATCCGGCCCCTCGCCGCGAAGTCGCCGACGTGCGCGACTGGA
TGCTCGCCGAAGCAGCCGAATGCGATGCGGCGATGCATGCGCACGATACGACGTCGGTCTGA

Upstream 100 bases:

>100_bases
CTACTCGGCCCCGTCCTCCGCGATCACCCAGCCTCCATTGAACTTGACGCGTGCCGCGTTCTCAACCAATCTTGCTGCTC
CGTTCGCATGAGTCACGCGC

Downstream 100 bases:

>100_bases
GTCGGCGGTGCGGATGCCCGTGACCACCGGCAACCGCCATCGCCCCTTCACATTCCCATGACGCATGCCAACCTGACCGA
ATGATCGCTTGAGCCGACGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVTLLQRGYRKIAFTPEGERLFRVADAAL
LGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPGIDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGA
LRLFDEIVVPVAHPALAARPVTNAAALADHVLLEFDGPPQPQLQWHAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
IALGRLALVAPMLADGRLAVLGPHTQTLSQTYGYWLFQHDPAPRREVADVRDWMLAEAAECDAAMHAHDTTSV

Sequences:

>Translated_313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVTLLQRGYRKIAFTPEGERLFRVADAAL
LGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPGIDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGA
LRLFDEIVVPVAHPALAARPVTNAAALADHVLLEFDGPPQPQLQWHAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
IALGRLALVAPMLADGRLAVLGPHTQTLSQTYGYWLFQHDPAPRREVADVRDWMLAEAAECDAAMHAHDTTSV
>Mature_312_residues
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVTLLQRGYRKIAFTPEGERLFRVADAALL
GLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPGIDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGAL
RLFDEIVVPVAHPALAARPVTNAAALADHVLLEFDGPPQPQLQWHAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQGI
ALGRLALVAPMLADGRLAVLGPHTQTLSQTYGYWLFQHDPAPRREVADVRDWMLAEAAECDAAMHAHDTTSV

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=296, Percent_Identity=31.7567567567568, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788706, Length=271, Percent_Identity=29.8892988929889, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=30.7958477508651, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=28.3464566929134, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081978, Length=264, Percent_Identity=29.5454545454545, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI145693193, Length=247, Percent_Identity=27.9352226720648, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787589, Length=231, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI157672245, Length=268, Percent_Identity=29.4776119402985, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33795; Mature: 33664

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVTLLQRGY
CCCCCEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKIAFTPEGERLFRVADAALLGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
ARLPGIDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGALRLFDEIVVPVAHPALAARP
HCCCCCEEEEECCCCEEEEEECCEEECEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCC
VTNAAALADHVLLEFDGPPQPQLQWHAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
CCHHHHHHHEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCEEEECHHHHHHHHHHCCCC
IALGRLALVAPMLADGRLAVLGPHTQTLSQTYGYWLFQHDPAPRREVADVRDWMLAEAAE
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHH
CDAAMHAHDTTSV
HHHHHHCCCCCCC
>Mature Secondary Structure 
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVTLLQRGY
CCCCEECCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKIAFTPEGERLFRVADAALLGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
ARLPGIDLRVAANDKVLDLRAEGIDLGIRYCPRERAPAGALRLFDEIVVPVAHPALAARP
HCCCCCEEEEECCCCEEEEEECCEEECEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCC
VTNAAALADHVLLEFDGPPQPQLQWHAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
CCHHHHHHHEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCEEEECHHHHHHHHHHCCCC
IALGRLALVAPMLADGRLAVLGPHTQTLSQTYGYWLFQHDPAPRREVADVRDWMLAEAAE
HHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHH
CDAAMHAHDTTSV
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]