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The map label for this gene is ynfL [H]

Identifier: 116690182

GI number: 116690182

Start: 2406957

End: 2407883

Strand: Direct

Name: ynfL [H]

Synonym: Bcen2424_2161

Alternate gene names: 116690182

Gene position: 2406957-2407883 (Clockwise)

Preceding gene: 116690178

Following gene: 116690194

Centisome position: 69.09

GC content: 68.93

Gene sequence:

>927_bases
ATGCCGACGCCGGGAGGAGACACCGTGGAACTGAGGCAGTTGCGCTACTTCGTGGCGGTCGCCGAGGAGCTGCATTTCGG
GCGCGCGGCGAAGCGCCTGTTCATCTCGCAGCCGGCGCTGAGTTTCGACATCCGCAAGTTCGAGGACGCGCTCGGCGTGC
AGCTGTTCTCGCGCACCAACAAGACGGTCGCGCTGACCAACGCGGGCGAGGTGCTGCTCGGCGAAGCGCGCCGGCTGCTG
CTGCAGGCGGACGAAGCCGAGCGCCTGACGGTGCGCTCCGCGTCGGGGCTCGCCGGCCGGCTGCGGATCGGCTTCGTCCA
TTCAATGCTGTATCGCGGGCTGCCCGACGCCGTGCGGCGCTTCGAAGCCGACTATCCGGGCGTCGAGGTCGTGCTGAGCG
AGATGAACACGCACGCGCAGGTGCAGGCGATCCAGCGCGGCCAGATCGATCTCGGCTATGCGCACTGGGGCCACTTCCCG
CCCGAGGTCGAATCGGTGGCCGTCTATGCGGAGCCGTTCGTGTGCTGCCTGCCGGCCGCGCATCCACTGGCGCGGCGCAG
GCAGGTCGCGCTCGCCACGCTCGCGACCGAACCGTTCATCCTGTTTCCGCGCGACGCCGCGCCGCACTATCACGACCTGA
TCATCGCGCAGTGCGTGAACGCGGGATTCAGTCCGCTGATCCGCCACGAGGCGCGGCTGTGGCAAACGATCCTGTCGATG
ATCGAGTTCGGGATGGGCATCGCACTGGTGCCGCGCGTGCTGCAGCAGGCGAAAAGCGATCGGCTCGCGTTCCTGCCGCT
GAAGGATGCGTCGCTCGAATCGCGCACGCTCGCGCTGAAACGCAGCGGCACCGCCGAACCGGTGGCGCTGCGCTTTGCCG
GCTACGTGCGCGCGTCGATCGACGCGCTGCCTGCGCTGGCGGGCTGA

Upstream 100 bases:

>100_bases
CGCCCGGCCGGTCCTGCGTCAAGCCTCCCGCAGAGCGTAGGCGCGCGATCGATAAGCGTCCAATACAGGATTTGTCCGGT
ACGATAGAGATCGCTTATCG

Downstream 100 bases:

>100_bases
GCGCGCGGCTCGCGATCCGGTGCTTGCGGCTATTTGAAAAAATTTGCAGAAAGGGCTTTACGAGCCCCAAAATCTCTGAC
ATAATTTGATTCTTCTTTGG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MPTPGGDTVELRQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDALGVQLFSRTNKTVALTNAGEVLLGEARRLL
LQADEAERLTVRSASGLAGRLRIGFVHSMLYRGLPDAVRRFEADYPGVEVVLSEMNTHAQVQAIQRGQIDLGYAHWGHFP
PEVESVAVYAEPFVCCLPAAHPLARRRQVALATLATEPFILFPRDAAPHYHDLIIAQCVNAGFSPLIRHEARLWQTILSM
IEFGMGIALVPRVLQQAKSDRLAFLPLKDASLESRTLALKRSGTAEPVALRFAGYVRASIDALPALAG

Sequences:

>Translated_308_residues
MPTPGGDTVELRQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDALGVQLFSRTNKTVALTNAGEVLLGEARRLL
LQADEAERLTVRSASGLAGRLRIGFVHSMLYRGLPDAVRRFEADYPGVEVVLSEMNTHAQVQAIQRGQIDLGYAHWGHFP
PEVESVAVYAEPFVCCLPAAHPLARRRQVALATLATEPFILFPRDAAPHYHDLIIAQCVNAGFSPLIRHEARLWQTILSM
IEFGMGIALVPRVLQQAKSDRLAFLPLKDASLESRTLALKRSGTAEPVALRFAGYVRASIDALPALAG
>Mature_307_residues
PTPGGDTVELRQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDALGVQLFSRTNKTVALTNAGEVLLGEARRLLL
QADEAERLTVRSASGLAGRLRIGFVHSMLYRGLPDAVRRFEADYPGVEVVLSEMNTHAQVQAIQRGQIDLGYAHWGHFPP
EVESVAVYAEPFVCCLPAAHPLARRRQVALATLATEPFILFPRDAAPHYHDLIIAQCVNAGFSPLIRHEARLWQTILSMI
EFGMGIALVPRVLQQAKSDRLAFLPLKDASLESRTLALKRSGTAEPVALRFAGYVRASIDALPALAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=263, Percent_Identity=37.6425855513308, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI2367136, Length=263, Percent_Identity=36.8821292775665, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1788887, Length=302, Percent_Identity=32.1192052980132, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI157672245, Length=302, Percent_Identity=30.4635761589404, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=30.3846153846154, Blast_Score=118, Evalue=6e-28,
Organism=Escherichia coli, GI145693105, Length=287, Percent_Identity=30.3135888501742, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790208, Length=284, Percent_Identity=29.2253521126761, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788748, Length=241, Percent_Identity=28.6307053941909, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=25.609756097561, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1789173, Length=272, Percent_Identity=28.3088235294118, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787601, Length=262, Percent_Identity=29.3893129770992, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081904, Length=213, Percent_Identity=28.6384976525822, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788481, Length=199, Percent_Identity=31.1557788944724, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1788296, Length=186, Percent_Identity=26.8817204301075, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=251, Percent_Identity=24.7011952191235, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788297, Length=153, Percent_Identity=31.3725490196078, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994962, Length=67, Percent_Identity=47.7611940298507, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787589, Length=124, Percent_Identity=36.2903225806452, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1789204, Length=246, Percent_Identity=25.609756097561, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790262, Length=314, Percent_Identity=26.1146496815287, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33929; Mature: 33798

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTPGGDTVELRQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDALGVQLFSRTN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
KTVALTNAGEVLLGEARRLLLQADEAERLTVRSASGLAGRLRIGFVHSMLYRGLPDAVRR
CEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHH
FEADYPGVEVVLSEMNTHAQVQAIQRGQIDLGYAHWGHFPPEVESVAVYAEPFVCCLPAA
HCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEEHHCCCCCCCHHHEEEEECCCEEECCCC
HPLARRRQVALATLATEPFILFPRDAAPHYHDLIIAQCVNAGFSPLIRHEARLWQTILSM
CHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IEFGMGIALVPRVLQQAKSDRLAFLPLKDASLESRTLALKRSGTAEPVALRFAGYVRASI
HHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEEECCCCCCCCHHHHHHHHHHHH
DALPALAG
HHHCCCCC
>Mature Secondary Structure 
PTPGGDTVELRQLRYFVAVAEELHFGRAAKRLFISQPALSFDIRKFEDALGVQLFSRTN
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCC
KTVALTNAGEVLLGEARRLLLQADEAERLTVRSASGLAGRLRIGFVHSMLYRGLPDAVRR
CEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCHHHHHH
FEADYPGVEVVLSEMNTHAQVQAIQRGQIDLGYAHWGHFPPEVESVAVYAEPFVCCLPAA
HCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEEHHCCCCCCCHHHEEEEECCCEEECCCC
HPLARRRQVALATLATEPFILFPRDAAPHYHDLIIAQCVNAGFSPLIRHEARLWQTILSM
CHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
IEFGMGIALVPRVLQQAKSDRLAFLPLKDASLESRTLALKRSGTAEPVALRFAGYVRASI
HHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCHHEEEEECCCCCCCCHHHHHHHHHHHH
DALPALAG
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]