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The map label for this gene is shiA [H]

Identifier: 116690179

GI number: 116690179

Start: 2402345

End: 2403757

Strand: Reverse

Name: shiA [H]

Synonym: Bcen2424_2158

Alternate gene names: 116690179

Gene position: 2403757-2402345 (Counterclockwise)

Preceding gene: 116690180

Following gene: 116690176

Centisome position: 69.0

GC content: 68.93

Gene sequence:

>1413_bases
ATGAGCCGTCCGCAATCCCCGCCCGCAGCACAACCGCGGCCCGGCCGCGCCGCCCTCGCGGCGTTCGTCGGCACCACGAT
CGAGTGGTACGACTTCTACATCTACGGCACGGCCGCGGCGCTCGTGTTCGGCAAGGTGTTCTTTTCGAGCACGATGGACC
CCGGCGTCGCGACGCTGCTCGCGTTCGTCACGTTCTGGGCCGGCTTCGCCGCGCGGCCGCTCGGCGGCATCGTGTTCGGC
CATCTCGGCGATCGCGTCGGCCGCAAGACCGCGCTCGTGATCACGCTGGTGATGATGGGGCTCGCGACGACCGGCATCGG
CCTGCTGCCCGGTTACGCGTCGATCGGCGTGTGGGCGCCGGCGGGCCTCGTCGCGCTACGCGTGCTGCAGGGCATCGCGG
TCGGCGGCGAGTGGGGCGGCGCGGTGCTGATCGCGAGCGAGAATGCGCCGAAGCATCGCAGCATCCTGTATGCGGCGTTC
GCGCAGCAAGGCTCGCCCACCGGCAACCTGCTGGCCACTGCGGCATTCTTCGCGCTGAGCGCGCTGCCGACGCCGTCGTT
CCTGATGTGGGGCTGGCGTATTCCGTTCCTGCTGTCGGCCGTGCTCGTGATCATCGGCATGGTGATCCGGCTGAGGCTCG
AGGAGTCGGTCGACATGCAGCGCGTGCTCGCGCGCAAGCGCACGGTGAAGCTGCCGCTGCGCGACGTCGTGCGCGACCAC
TGGGTCGTCGTGCTGCTCGCGGCCGGCACGCTGCCGGTGATCAACGTCACGTATTTCCGCAGCACGTTCGCTTTGTCGTG
GGCGACGAAGACGCTCGGCTACGCGCAGGGCACGTTCCTCGGCATCCTGTCGATCTCGCTCGTCGTGCAATTCCTGATGC
AGCCGGTCGGCGCGTGGTTCGTGTCGAAGGTCGACATGCGGCGCGCGATGTGCTGGATCCTGATTCCGGAAATCGTGCTG
ATGCCCGTGATGTTCCATGCGCTTGCGACGCGTTCGTACTGGGTCGCCGTCGCGGGCATGTGCATCTCGACGATTCCGTC
GGCGATGTTCTACGGCGCGGTCGGCGGCGTGCTCGCGCGCGTGTTTCCGGCCAACATCCGCTATACGGGCCTGTCGCTCG
CGTATCAGCTGAGCGCGCTGGTCGTCGGCGGCGGCACGCCGGTGCTCGCGCAGGCGATCCTCAATGCGACCGGCAGCATC
GTCGGCGTCGCGATCGCGTCGGGGCTGTACGCGCTCGTGTCGCTGGCGTGCATGCTGGCGCTGCTCAATCGCACCGGATA
TCGCGCCGACGAACTGTCGAGCGCCGAGCGCAGCGATGCGGCCGAGTGGGGCACCGAAGGCGCGAGCGACGATCGTCCGG
TCGGCGAGCCGGCGCAGCCGGACGATCGCGGCACGTTGAAACCGGCGGGTTGA

Upstream 100 bases:

>100_bases
GCCCGACTGACACGACGCCGCCGGCCGGCGGCGTGATGCCGGCCACCCGGGAAAGGCGCGCGCGAGTCGCGCCCCCGATC
ATCACAATATGGAGACATCG

Downstream 100 bases:

>100_bases
GCGGGGGAGGCCGGAGTGATTCGGCACGCTGGCGATTCGGCAATTCGGTGAGACGGCAATTCGCCGGCGCGGTAGCGCGG
TGATTCGGCGCTCCGTCGGC

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MSRPQSPPAAQPRPGRAALAAFVGTTIEWYDFYIYGTAAALVFGKVFFSSTMDPGVATLLAFVTFWAGFAARPLGGIVFG
HLGDRVGRKTALVITLVMMGLATTGIGLLPGYASIGVWAPAGLVALRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAF
AQQGSPTGNLLATAAFFALSALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLRLEESVDMQRVLARKRTVKLPLRDVVRDH
WVVVLLAAGTLPVINVTYFRSTFALSWATKTLGYAQGTFLGILSISLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVL
MPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNATGSI
VGVAIASGLYALVSLACMLALLNRTGYRADELSSAERSDAAEWGTEGASDDRPVGEPAQPDDRGTLKPAG

Sequences:

>Translated_470_residues
MSRPQSPPAAQPRPGRAALAAFVGTTIEWYDFYIYGTAAALVFGKVFFSSTMDPGVATLLAFVTFWAGFAARPLGGIVFG
HLGDRVGRKTALVITLVMMGLATTGIGLLPGYASIGVWAPAGLVALRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAF
AQQGSPTGNLLATAAFFALSALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLRLEESVDMQRVLARKRTVKLPLRDVVRDH
WVVVLLAAGTLPVINVTYFRSTFALSWATKTLGYAQGTFLGILSISLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVL
MPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNATGSI
VGVAIASGLYALVSLACMLALLNRTGYRADELSSAERSDAAEWGTEGASDDRPVGEPAQPDDRGTLKPAG
>Mature_469_residues
SRPQSPPAAQPRPGRAALAAFVGTTIEWYDFYIYGTAAALVFGKVFFSSTMDPGVATLLAFVTFWAGFAARPLGGIVFGH
LGDRVGRKTALVITLVMMGLATTGIGLLPGYASIGVWAPAGLVALRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAFA
QQGSPTGNLLATAAFFALSALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLRLEESVDMQRVLARKRTVKLPLRDVVRDHW
VVVLLAAGTLPVINVTYFRSTFALSWATKTLGYAQGTFLGILSISLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVLM
PVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNATGSIV
GVAIASGLYALVSLACMLALLNRTGYRADELSSAERSDAAEWGTEGASDDRPVGEPAQPDDRGTLKPAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=435, Percent_Identity=37.7011494252874, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789941, Length=384, Percent_Identity=39.5833333333333, Blast_Score=211, Evalue=7e-56,
Organism=Escherichia coli, GI1790550, Length=397, Percent_Identity=31.4861460957179, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1788942, Length=376, Percent_Identity=32.7127659574468, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI87082231, Length=285, Percent_Identity=25.9649122807018, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49856; Mature: 49725

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: PS00024 HEMOPEXIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRPQSPPAAQPRPGRAALAAFVGTTIEWYDFYIYGTAAALVFGKVFFSSTMDPGVATLL
CCCCCCCCCCCCCCCHHHHHHHHCCCEEHHEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
AFVTFWAGFAARPLGGIVFGHLGDRVGRKTALVITLVMMGLATTGIGLLPGYASIGVWAP
HHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCCCH
AGLVALRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAFAQQGSPTGNLLATAAFFALS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLRLEESVDMQRVLARKRTVKLPLRDVVRDH
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
WVVVLLAAGTLPVINVTYFRSTFALSWATKTLGYAQGTFLGILSISLVVQFLMQPVGAWF
HEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSKVDMRRAMCWILIPEIVLMPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNATGSIVGVAIASGLYALVSLACMLA
HHCCCCEEEHHHHHHEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLNRTGYRADELSSAERSDAAEWGTEGASDDRPVGEPAQPDDRGTLKPAG
HHHCCCCCHHHHCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRPQSPPAAQPRPGRAALAAFVGTTIEWYDFYIYGTAAALVFGKVFFSSTMDPGVATLL
CCCCCCCCCCCCCCHHHHHHHHCCCEEHHEEEEHHHHHHHHHHHHHHHCCCCHHHHHHH
AFVTFWAGFAARPLGGIVFGHLGDRVGRKTALVITLVMMGLATTGIGLLPGYASIGVWAP
HHHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHCCCCHHHCCCCH
AGLVALRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAFAQQGSPTGNLLATAAFFALS
HHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLRLEESVDMQRVLARKRTVKLPLRDVVRDH
CCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHH
WVVVLLAAGTLPVINVTYFRSTFALSWATKTLGYAQGTFLGILSISLVVQFLMQPVGAWF
HEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
VSKVDMRRAMCWILIPEIVLMPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNATGSIVGVAIASGLYALVSLACMLA
HHCCCCEEEHHHHHHEEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
LLNRTGYRADELSSAERSDAAEWGTEGASDDRPVGEPAQPDDRGTLKPAG
HHHCCCCCHHHHCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]