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The map label for this gene is folD [H]

Identifier: 116690161

GI number: 116690161

Start: 2377163

End: 2378020

Strand: Direct

Name: folD [H]

Synonym: Bcen2424_2140

Alternate gene names: 116690161

Gene position: 2377163-2378020 (Clockwise)

Preceding gene: 116690160

Following gene: 116690162

Centisome position: 68.23

GC content: 67.37

Gene sequence:

>858_bases
ATGACAGCCCTCCTCATCGACGGCAACGCCCTTTCGAAGACCTTGCGCGCCCAGGCCGCCGAACGCGCCGCCGCGCTGAC
CGCACGCGGCCACCAGCCCGGTCTCGCGGTGATCCTCGTCGGCGCCAACCCGGCGAGCGAAGTCTACGTGCGCAACAAGA
TCAAGGCGTGCGAGGACAACGGCTTCTTCTCGCTGAAGGATGCGTACCCGGCCACGCTGTCGGAAGCCGACCTGCTCGCG
CGCATCGACGAACTGAACCGCGACCCGAAGATCCACGGCATCCTCGTCCAGCTGCCGCTGCCCGCGCACATCGACAGCCA
CAAGGTGATCGAGGCGATCGCGCCGGAAAAGGACGTCGACGGCTTTCACGTCGCGAACGCCGGCGCGCTGATGACCGGCA
AGCCGCTGTTCCGCCCGTGCACACCGTACGGCGTGATGAAGATGTTCGAAGCGCACGGCATTGCGCTGCAAGGCGCGAAC
GCGGTCGTGATCGGCCGCTCAAACATCGTCGGCAAGCCGATGGCGATGATGCTGCTCGACGCCGGCGCGACCGTGACGAT
TTGCCACAGCAAGACGCGCGACCTCGCCGCGCATACGCGCGAGGCCGACATCGTGGTCGCCGCGGTCGGCAAGCGCAACA
TCCTGACCGCCGACATGGTGAAGCCGGGCGCGACGGTGATCGACGTCGGCATGAACCGTGACGACGCGGGCAAGCTGTGC
GGCGACGTCGATTTCGCGGGCGTGAAGGAAGTCGCCGGCTACATTACGCCGGTGCCGGGCGGCGTCGGCCCGATGACCAT
CACGATGCTGCTGATCAACACGATCGAATCCGCCGAACGCGCCGCCGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
CTGTCGGACGGCAGCAGGCGAACGGTATAATTACGTGCTTTGCTACGGCGCTTCGCGCGCCGTACGCCCGCATTCCAACT
TCCCGGCAGGACCCACCACC

Downstream 100 bases:

>100_bases
CGCTGCCTTTGCCGAGCCGGTGCGCGGTCCGCGCGCCGGCGCCTCGCCAGCCGCAATCGCGCGGCTGCATCGCGCCGGCC
GGCGCGCCGATGCACCTCGC

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MTALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDNGFFSLKDAYPATLSEADLLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFEAHGIALQGAN
AVVIGRSNIVGKPMAMMLLDAGATVTICHSKTRDLAAHTREADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTIESAERAAAAA

Sequences:

>Translated_285_residues
MTALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDNGFFSLKDAYPATLSEADLLA
RIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFEAHGIALQGAN
AVVIGRSNIVGKPMAMMLLDAGATVTICHSKTRDLAAHTREADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTIESAERAAAAA
>Mature_284_residues
TALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDNGFFSLKDAYPATLSEADLLAR
IDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVDGFHVANAGALMTGKPLFRPCTPYGVMKMFEAHGIALQGANA
VVIGRSNIVGKPMAMMLLDAGATVTICHSKTRDLAAHTREADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLCG
DVDFAGVKEVAGYITPVPGGVGPMTITMLLINTIESAERAAAAA

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=47.2789115646259, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=45.578231292517, Blast_Score=232, Evalue=2e-61,
Organism=Homo sapiens, GI222136639, Length=290, Percent_Identity=42.4137931034483, Blast_Score=221, Evalue=5e-58,
Organism=Homo sapiens, GI36796743, Length=223, Percent_Identity=27.8026905829596, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=52.3297491039427, Blast_Score=281, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=295, Percent_Identity=42.7118644067797, Blast_Score=204, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=47.2027972027972, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=300, Percent_Identity=41.3333333333333, Blast_Score=225, Evalue=5e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=295, Percent_Identity=25.7627118644068, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=45.3287197231834, Blast_Score=253, Evalue=9e-68,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=45.3287197231834, Blast_Score=253, Evalue=9e-68,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=45.3287197231834, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=45.3287197231834, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=47.2789115646259, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=47.2789115646259, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 29787; Mature: 29656

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDN
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEHHCCCEECCC
GFFSLKDAYPATLSEADLLARIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALMTGKPLFRPCTPYGVMKMFEAHGIALQGANAVVIGRSNIVGKPMAMMLLD
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCEEEECCCEEEEECCCCCCCCCEEEEEC
AGATVTICHSKTRDLAAHTREADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCHHHC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTIESAERAAAAA
CCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TALLIDGNALSKTLRAQAAERAAALTARGHQPGLAVILVGANPASEVYVRNKIKACEDN
EEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHEEEHHCCCEECCC
GFFSLKDAYPATLSEADLLARIDELNRDPKIHGILVQLPLPAHIDSHKVIEAIAPEKDVD
CEEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCCCC
GFHVANAGALMTGKPLFRPCTPYGVMKMFEAHGIALQGANAVVIGRSNIVGKPMAMMLLD
CEEEECCCEEEECCCCCCCCCCHHHHHHHHHCCEEEECCCEEEEECCCCCCCCCEEEEEC
AGATVTICHSKTRDLAAHTREADIVVAAVGKRNILTADMVKPGATVIDVGMNRDDAGKLC
CCCEEEEEECCCHHHHHHCCCCCEEEEECCCCCEEEHHCCCCCCEEEEECCCCCCCHHHC
GDVDFAGVKEVAGYITPVPGGVGPMTITMLLINTIESAERAAAAA
CCCCCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA