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The map label for this gene is pdhB [H]

Identifier: 116690157

GI number: 116690157

Start: 2369096

End: 2370745

Strand: Reverse

Name: pdhB [H]

Synonym: Bcen2424_2136

Alternate gene names: 116690157

Gene position: 2370745-2369096 (Counterclockwise)

Preceding gene: 116690158

Following gene: 116690156

Centisome position: 68.05

GC content: 67.45

Gene sequence:

>1650_bases
ATGAGTCAAGCGATCGAAGTGAAGGTGCCGGATATCGGCGATTACAAGGACGTGCCGGTCATCGAGATCGGCGTGAAGGT
GGGCGATACGGTCGAGCCCGAGCAGTCGCTCGTCACGCTCGAGTCGGACAAGGCGACGATGGACGTGCCGAGCCCGGTCG
GCGGCGTCGTGAAGGAAATCAAGGTCAAGGTCGGCGATTCCGTGTCGGAAGGCTCGCTGATCATCCTGCTCGAAGGCGGC
GCAGGCGCCCAGGCGAACGGCGCGGCCGCGCCGGCCGCGGCTCCGGCTCCGGCAGCAGCACCGGCACCGGCACCCGCAGC
TCCCGCTGCCGCGCCGGCGGCGTCCGGCGGCACGCTGGAAGTGAAGGTGCCCGACATCGGCGACTACAAGGACGTCCCGG
TGATCGAGATCGGCGTGAAGGTCGGCGACACGGTCGAGAAGGAACAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACG
ATGGACGTGCCGAGCCCGGCCGCCGGCGTCGTGAAGGACATCAAGGTCAAGGTGGGCGATTCGGTGTCGGAAGGCACGCT
GATCGTGCTGCTCGAAGCCGCCGGCGCACCGGCTGCCGCACCGCAGGCCAGCGCACCGGCTCCGGCCGCTGCCGCACCGG
CGCCTGCTGCTGCACCGGCACCCGCGCCTGCGAAGGCTGCGCCGGCACCGGCTGCCGCCGCCCCGGCCGCCGCGCCGGCG
GGCGAATACCGCGCCAGCCACGCATCGCCGTCGGTCCGCAAGTTCGCACGCGAACTCGGCGTCGACGTCGCGCGTGTGCA
GGGTTCGGGTCCGAAGGGCCGCATTACGAAGGAAGACGTCACGGGCTTCGTGAAGGGCGTGATGACCGGCCAGCGCGCGG
CACCCGCCGCGGCAGCCGCACCGGCAGGCGGCGGCGAGCTGAACCTGCTGCCGTGGCCGAAGGTCGACTTCTCGAAGTTC
GGCCCGTTCGAGGCGAAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACCTGCATCGCAACTGGGTGATGATCCC
GCACGTCACGAACAACGACGAAGCGGACATCACCGAGCTCGAAGCGCTGCGCGTCCAGCTGAACAAGGAGCACGAAAAGG
CGGGCGTGAAGTTCACGATGCTCGCGTTCGTGATCAAGGCAGTCGTCGCCGCGCTGAAGAAATTCCCGACCTTCAACGCG
AGCCTCGACGGCGACAACCTCGTGTTCAAGCAGTACTTCCACGTCGGTTTCGCGGCCGATACGCCGAACGGCCTCGTCGT
GCCGGTGATCCGCGATGCGGACAAGAAGGGCCTCGTCGACATCGCGAAGGAAATGAGCGACCTGTCGAAGGCCGCGCGCG
AAGGCAAGCTGAAGCCGGACCAGATGCAAGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGGATCGGCGGGACGAACTTC
ACGCCGATCATCAACGCGCCGGAAGTGGCGATCCTCGGGTTGTCGCGCGGCCAGATGAAGCCGGTGTGGGACGGCAAGCA
GTTCGTGCCGCGGCTCATGCTGCCGCTGTCGCTGTCGTATGACCATCGCGTCATCGATGGCGCGGAAGCCGCGCGGTTCA
ATGCGTATCTCGGGGCGTTGCTCGGGGATTTCCGTCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
ATCCGTCCAAGCCCAACCCGATGACCGTTTAACGCACACGCCGTGTGATGCCGCGCGCCGCTCCCGCGAGGAGCGCGCGC
GGCCATGGAGACAGAAACAG

Downstream 100 bases:

>100_bases
TGAGATGAGGGGCTCGCGGGGGCGTGGGTTTGTTTTTGGTTCTCCACGCCCTTGTCGTTGAACCTGTTTTGCCAACGGTC
TGCCAGCGTCGCCCCTGCGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 549; Mature: 548

Protein sequence:

>549_residues
MSQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEIKVKVGDSVSEGSLIILLEGG
AGAQANGAAAPAAAPAPAAAPAPAPAAPAAAPAASGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKAT
MDVPSPAAGVVKDIKVKVGDSVSEGTLIVLLEAAGAPAAAPQASAPAPAAAAPAPAAAPAPAPAKAAPAPAAAAPAAAPA
GEYRASHASPSVRKFARELGVDVARVQGSGPKGRITKEDVTGFVKGVMTGQRAAPAAAAAPAGGGELNLLPWPKVDFSKF
GPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNA
SLDGDNLVFKQYFHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMSDLSKAAREGKLKPDQMQGGCFSISSLGGIGGTNF
TPIINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLGDFRRIIL

Sequences:

>Translated_549_residues
MSQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEIKVKVGDSVSEGSLIILLEGG
AGAQANGAAAPAAAPAPAAAPAPAPAAPAAAPAASGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKAT
MDVPSPAAGVVKDIKVKVGDSVSEGTLIVLLEAAGAPAAAPQASAPAPAAAAPAPAAAPAPAPAKAAPAPAAAAPAAAPA
GEYRASHASPSVRKFARELGVDVARVQGSGPKGRITKEDVTGFVKGVMTGQRAAPAAAAAPAGGGELNLLPWPKVDFSKF
GPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNA
SLDGDNLVFKQYFHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMSDLSKAAREGKLKPDQMQGGCFSISSLGGIGGTNF
TPIINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLGDFRRIIL
>Mature_548_residues
SQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEIKVKVGDSVSEGSLIILLEGGA
GAQANGAAAPAAAPAPAAAPAPAPAAPAAAPAASGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDSVSEGTLIVLLEAAGAPAAAPQASAPAPAAAAPAPAAAPAPAPAKAAPAPAAAAPAAAPAG
EYRASHASPSVRKFARELGVDVARVQGSGPKGRITKEDVTGFVKGVMTGQRAAPAAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYFHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMSDLSKAAREGKLKPDQMQGGCFSISSLGGIGGTNFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLGDFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=448, Percent_Identity=29.9107142857143, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI31711992, Length=425, Percent_Identity=32.7058823529412, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI203098816, Length=488, Percent_Identity=27.4590163934426, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI203098753, Length=424, Percent_Identity=28.5377358490566, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=33.920704845815, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=36.6013071895425, Blast_Score=97, Evalue=4e-20,
Organism=Escherichia coli, GI1786305, Length=548, Percent_Identity=54.9270072992701, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=30.4147465437788, Blast_Score=185, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=29.0094339622642, Blast_Score=172, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=29.8876404494382, Blast_Score=148, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=205, Percent_Identity=37.0731707317073, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=32.3529411764706, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=416, Percent_Identity=29.5673076923077, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=430, Percent_Identity=29.0697674418605, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=32.7102803738318, Blast_Score=179, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=32.3275862068966, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=32.3275862068966, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.7826086956522, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56302; Mature: 56171

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEI
CCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHH
KVKVGDSVSEGSLIILLEGGAGAQANGAAAPAAAPAPAAAPAPAPAAPAAAPAASGGTLE
HHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGD
EECCCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCHHHHHEEEEEEECC
SVSEGTLIVLLEAAGAPAAAPQASAPAPAAAAPAPAAAPAPAPAKAAPAPAAAAPAAAPA
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEYRASHASPSVRKFARELGVDVARVQGSGPKGRITKEDVTGFVKGVMTGQRAAPAAAAA
CHHHCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCC
PAGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITEL
CCCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHH
EALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYFHVGFAAD
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHHEEEECC
TPNGLVVPVIRDADKKGLVDIAKEMSDLSKAAREGKLKPDQMQGGCFSISSLGGIGGTNF
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCC
TPIINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGAL
CEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCEEECCCHHHHHHHHHHHH
LGDFRRIIL
HHHHHHHCC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEI
CCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHH
KVKVGDSVSEGSLIILLEGGAGAQANGAAAPAAAPAPAAAPAPAPAAPAAAPAASGGTLE
HHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGD
EECCCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCHHHHHEEEEEEECC
SVSEGTLIVLLEAAGAPAAAPQASAPAPAAAAPAPAAAPAPAPAKAAPAPAAAAPAAAPA
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEYRASHASPSVRKFARELGVDVARVQGSGPKGRITKEDVTGFVKGVMTGQRAAPAAAAA
CHHHCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHCC
PAGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITEL
CCCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHH
EALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYFHVGFAAD
HHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHEEHHHHEEEECC
TPNGLVVPVIRDADKKGLVDIAKEMSDLSKAAREGKLKPDQMQGGCFSISSLGGIGGTNF
CCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCC
TPIINAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGAL
CEEECCCCEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCEEECCCHHHHHHHHHHHH
LGDFRRIIL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]