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The map label for this gene is lysS [H]

Identifier: 116690139

GI number: 116690139

Start: 2351471

End: 2352997

Strand: Direct

Name: lysS [H]

Synonym: Bcen2424_2118

Alternate gene names: 116690139

Gene position: 2351471-2352997 (Clockwise)

Preceding gene: 116690138

Following gene: 116690140

Centisome position: 67.5

GC content: 65.03

Gene sequence:

>1527_bases
ATGACCGAACCGACCCAAACGCAGCCCGCCGTCACGGCGGACGAAAACCAGATCATCGCCGAGCGCCGCGAGAAGCTGCG
CGCGTTGCGCGAGCAAGGCGTCGCCTACCCGAACGATTTCCGCCCCGAGCACCACGCAGCCGACCTGCAGGCCAAATTCG
CCGATTCGGACAAGGCCGCGCTCGAAGCGAACCCGGTCGAGGTGTCGGTGGCCGGCCGCATGATGCTCAAGCGCGTGATG
GGCAAGGCGAGCTTCGCGACGGTGCAGGACGGCTCGGGCCAGATCCAGTTCTTCGTGACGCCGAACGACGTCGGCGCCGA
CACCTACGACGCGTTCAAGAAGTGGGACCTCGGCGACATCGTCGCCGCGCGCGGCGTGCTGTTCCGCACCAACAAGGGCG
AGCTGTCGGTCCAGTGCAAGGAGCTGCGTCTGCTGTCGAAGGCGCTGCGCCCGCTGCCGGACAAGTTCCACGGGCTGTCC
GACCAGGAAATGCGCTACCGCCAGCGCTACGTCGACCTGATCGTCACGCCGGAAACGCGCGACACGTTCCGCGCCCGCAC
GAAGACGATCGCGTCGATCCGCAAGTTCATGGACAACGCCGACTTCATGGAAGTCGAGACGCCGATGCTGCACCCGATCC
CGGGCGGCGCGGCCGCGAAGCCGTTCGTCACGCATCACAATGCGCTCGACATGCAGATGTTCCTGCGCATCGCGCCGGAG
CTGTACCTGAAGCGCCTGATCGTCGGCGGCTTCGAGCGCGTGTTCGAGATCAACCGCAATTTCCGGAATGAAGGCGTGTC
GCCGCGTCACAATCCGGAATTCACGATGATGGAGTTCTACGCCGCGTACACCGATTACCGCTGGCTGATGGACTTCACCG
AGCAACTGATCCGCCAGGCGGCGATCGATGCGCTCGGCACCGCGACGATCCAGTATCAAGGCCGTGAACTCGACCTCGCG
AAGCCGTTCCATCGCCTGACGATCACGCAGGCGATCCAGAAGTACGCACCGGACTACACCGACGGCCAGCTGTCGGACGA
CGCGTTCCTGCGCACCGAACTGAAGCGCTTCGGCGTCGACGTGTCGCAGCCGGCGTTCCTGAACGCCGGCATCGGCGCCC
TTCAGCTCGCGCTGTTCGAGGAAACGGCCGAAGCGCAGCTGTGGGAACCGACGTTCATCATCGACTACCCGGTCGAGGTG
TCGCCGCTCGCACGCGCATCGGATACGGTGCCGGGCATCACCGAGCGTTTCGAGCTGTTCATGACCGGCCGCGAGATCGC
GAACGGCTTCTCGGAGCTGAACGATCCGGAAGACCAGGCCGCGCGCTTCAAGAAGCAGGTCGAGCAAAAGGATGCGGGCG
ACGAGGAAGCGATGTTCTTCGACGCCGACTACATCCGCGCGCTCGAATACGGGATGCCTCCGACCGGCGGCTGCGGGATC
GGCATCGACCGTCTCGTGATGCTGCTGACCGACAGCCCGACGATTCGCGACGTGCTGCTGTTCCCGCATCTGCGCCGCGA
AGACTGA

Upstream 100 bases:

>100_bases
AGCGCGAGCCTCAAACAGGGCGTGTAAGCGCCGCGGCGCGGCCGGCCCGCGCCGCCCTTTCCGTTTGCGTTGCCGCACCC
CACTCCGAATTCCGACCATC

Downstream 100 bases:

>100_bases
CGGCCGCGCCCGCGTGTGCGCCGCCCCCCGTGGCGCGCACGCGTCGGCTTTGTAACGACGCGTAAATCCCGCCAGCCGGC
GTCTGCCGGCCTTCGATCCT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 508; Mature: 507

Protein sequence:

>508_residues
MTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPEHHAADLQAKFADSDKAALEANPVEVSVAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPNDVGADTYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLS
DQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLA
KPFHRLTITQAIQKYAPDYTDGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEV
SPLARASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED

Sequences:

>Translated_508_residues
MTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPEHHAADLQAKFADSDKAALEANPVEVSVAGRMMLKRVM
GKASFATVQDGSGQIQFFVTPNDVGADTYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLS
DQEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLA
KPFHRLTITQAIQKYAPDYTDGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEV
SPLARASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
GIDRLVMLLTDSPTIRDVLLFPHLRRED
>Mature_507_residues
TEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPEHHAADLQAKFADSDKAALEANPVEVSVAGRMMLKRVMG
KASFATVQDGSGQIQFFVTPNDVGADTYDAFKKWDLGDIVAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLSD
QEMRYRQRYVDLIVTPETRDTFRARTKTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPEL
YLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQAAIDALGTATIQYQGRELDLAK
PFHRLTITQAIQKYAPDYTDGQLSDDAFLRTELKRFGVDVSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEVS
PLARASDTVPGITERFELFMTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGIG
IDRLVMLLTDSPTIRDVLLFPHLRRED

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=514, Percent_Identity=40.0778210116732, Blast_Score=356, Evalue=2e-98,
Organism=Homo sapiens, GI194272210, Length=514, Percent_Identity=40.0778210116732, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI40789249, Length=330, Percent_Identity=24.5454545454545, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1789256, Length=506, Percent_Identity=58.498023715415, Blast_Score=608, Evalue=1e-175,
Organism=Escherichia coli, GI1790571, Length=493, Percent_Identity=59.2292089249493, Blast_Score=603, Evalue=1e-173,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=31.7073170731707, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788173, Length=284, Percent_Identity=24.6478873239437, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=531, Percent_Identity=40.8662900188324, Blast_Score=363, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17535925, Length=531, Percent_Identity=40.8662900188324, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI71994340, Length=498, Percent_Identity=42.1686746987952, Blast_Score=362, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17551876, Length=475, Percent_Identity=22.9473684210526, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=285, Percent_Identity=24.2105263157895, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=519, Percent_Identity=42.0038535645472, Blast_Score=369, Evalue=1e-103,
Organism=Saccharomyces cerevisiae, GI6324256, Length=550, Percent_Identity=30.9090909090909, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6321807, Length=455, Percent_Identity=24.1758241758242, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325153, Length=325, Percent_Identity=24, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640849, Length=518, Percent_Identity=41.3127413127413, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640851, Length=518, Percent_Identity=41.3127413127413, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24584738, Length=318, Percent_Identity=25.7861635220126, Blast_Score=91, Evalue=3e-18,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57558; Mature: 57427

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPEHHAADLQAKFADSDKAA
CCCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHCCCCCCCE
LEANPVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGADTYDAFKKWDLGDI
EECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCHHHH
VAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHEEEEECCCCH
DTFRARTKTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTDGQLSDDAFLRTELKRFGVD
HHHHHCCEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
VSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEVSPLARASDTVPGITERFELF
CCCCCHHHCCHHHHHHHHHHHHHHCEECCCEEEEECCCCCCCCHHCCCCCCCHHHHHHHH
MTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHEEHHCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEPTQTQPAVTADENQIIAERREKLRALREQGVAYPNDFRPEHHAADLQAKFADSDKAA
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCHHHCCCCCCCE
LEANPVEVSVAGRMMLKRVMGKASFATVQDGSGQIQFFVTPNDVGADTYDAFKKWDLGDI
EECCCEEEEHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHCCCHHHH
VAARGVLFRTNKGELSVQCKELRLLSKALRPLPDKFHGLSDQEMRYRQRYVDLIVTPETR
HHHCCEEEECCCCCEEEHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHEEEEECCCCH
DTFRARTKTIASIRKFMDNADFMEVETPMLHPIPGGAAAKPFVTHHNALDMQMFLRIAPE
HHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHH
LYLKRLIVGGFERVFEINRNFRNEGVSPRHNPEFTMMEFYAAYTDYRWLMDFTEQLIRQA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIDALGTATIQYQGRELDLAKPFHRLTITQAIQKYAPDYTDGQLSDDAFLRTELKRFGVD
HHHHHCCEEEEECCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
VSQPAFLNAGIGALQLALFEETAEAQLWEPTFIIDYPVEVSPLARASDTVPGITERFELF
CCCCCHHHCCHHHHHHHHHHHHHHCEECCCEEEEECCCCCCCCHHCCCCCCCHHHHHHHH
MTGREIANGFSELNDPEDQAARFKKQVEQKDAGDEEAMFFDADYIRALEYGMPPTGGCGI
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHCCCCCCCCCCC
GIDRLVMLLTDSPTIRDVLLFPHLRRED
CHHHHEEHHCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA