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The map label for this gene is rotA [H]

Identifier: 116690101

GI number: 116690101

Start: 2310676

End: 2311251

Strand: Direct

Name: rotA [H]

Synonym: Bcen2424_2080

Alternate gene names: 116690101

Gene position: 2310676-2311251 (Clockwise)

Preceding gene: 116690100

Following gene: 116690102

Centisome position: 66.32

GC content: 63.89

Gene sequence:

>576_bases
ATGAAACGTCTGTTGCTGGCGCTTGGCGGCGCCGCCCTTCTCGCGACCGCGCCCGCGTTCGCGCAAACGGCCACCGCGCA
CCCCGTCGTGCAGCTGAAGACCTCGCAGGGCGACATCCGCGTCGAGCTCTATCCGGAGAAGGCGCCGAAATCCGTCGCCA
ACTTCCTCGATTACGTGAAGGCCGGCCAGTACAACGGCACGATCTTCCATCGCGTGATCAAGGGCTTCATGATCCAGGGC
GGCGGCTACAAGACCAACTTCGAGGAAAAGCCGACCCGCGCGCCGATCCCGCTGGAAAGCCGCAATGGTCTGAAGAACCT
GACGGGCACGATCGCGATGGCGCGCACGAGCGATCCGAATTCGGCCACCGCGCAGTTCTTCATCAATACGGTCGACAACG
GCGGCCTCGACTATCCGAACCCGGACGGCAACGGCTATGCGGTGTTCGGCAAGGTCGTGTCCGGCCTCGACGTCGTGAAG
AAGATCGAAGGCGTGGCGACGACCTCGCGCGGCCCGATGCAGGACGTACCGGCGCAACCGGTCGTGATCGAATCGGCGAG
CATCGTCTCGAAGTAA

Upstream 100 bases:

>100_bases
GTTCCAGTTCAGCGGTCCGTCGGGCGCACTGGCGGCGCCGTACGTCGCGCCGTCGCAATAAGCGGCGCGACCTTCCTTCC
CTCCCAACCTGAGGATCGCA

Downstream 100 bases:

>100_bases
GCACCTGCCGGCACGTCCGGCGCCACACGCGGCCGCGTCGCACGACCGGCCGCGTGCCATTTAAACCGCCTCACCGAAGG
AATTCATCATGGTTGAACTG

Product: peptidylprolyl isomerase

Products: NA

Alternate protein names: PPIase A; Cyclophilin A; Rotamase A [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKRLLLALGGAALLATAPAFAQTATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVKAGQYNGTIFHRVIKGFMIQG
GGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNGGLDYPNPDGNGYAVFGKVVSGLDVVK
KIEGVATTSRGPMQDVPAQPVVIESASIVSK

Sequences:

>Translated_191_residues
MKRLLLALGGAALLATAPAFAQTATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVKAGQYNGTIFHRVIKGFMIQG
GGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNGGLDYPNPDGNGYAVFGKVVSGLDVVK
KIEGVATTSRGPMQDVPAQPVVIESASIVSK
>Mature_191_residues
MKRLLLALGGAALLATAPAFAQTATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVKAGQYNGTIFHRVIKGFMIQG
GGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPNSATAQFFINTVDNGGLDYPNPDGNGYAVFGKVVSGLDVVK
KIEGVATTSRGPMQDVPAQPVVIESASIVSK

Specific function: PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides [H]

COG id: COG0652

COG function: function code O; Peptidyl-prolyl cis-trans isomerase (rotamase) - cyclophilin family

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase cyclophilin-type domain [H]

Homologues:

Organism=Homo sapiens, GI19557636, Length=170, Percent_Identity=33.5294117647059, Blast_Score=87, Evalue=7e-18,
Organism=Homo sapiens, GI22547212, Length=162, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI7657473, Length=162, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI22547215, Length=162, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI24308049, Length=168, Percent_Identity=34.5238095238095, Blast_Score=82, Evalue=2e-16,
Organism=Homo sapiens, GI7706339, Length=171, Percent_Identity=35.672514619883, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI5454154, Length=185, Percent_Identity=34.0540540540541, Blast_Score=74, Evalue=7e-14,
Organism=Homo sapiens, GI4758950, Length=201, Percent_Identity=32.3383084577114, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI64276486, Length=135, Percent_Identity=35.5555555555556, Blast_Score=68, Evalue=6e-12,
Organism=Escherichia coli, GI1789763, Length=190, Percent_Identity=54.2105263157895, Blast_Score=183, Evalue=5e-48,
Organism=Escherichia coli, GI1786736, Length=162, Percent_Identity=54.9382716049383, Blast_Score=183, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI71980594, Length=160, Percent_Identity=35.625, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI71980590, Length=144, Percent_Identity=36.8055555555556, Blast_Score=90, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17539496, Length=154, Percent_Identity=32.4675324675325, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17532641, Length=162, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17559074, Length=160, Percent_Identity=33.75, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17566266, Length=158, Percent_Identity=33.5443037974684, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17552780, Length=200, Percent_Identity=31, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565860, Length=153, Percent_Identity=33.9869281045752, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17506307, Length=166, Percent_Identity=34.3373493975904, Blast_Score=66, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71997782, Length=135, Percent_Identity=32.5925925925926, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6323562, Length=160, Percent_Identity=36.25, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6320359, Length=145, Percent_Identity=35.1724137931034, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922376, Length=170, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI17986117, Length=170, Percent_Identity=32.3529411764706, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI19922912, Length=175, Percent_Identity=33.7142857142857, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI45549139, Length=168, Percent_Identity=36.3095238095238, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI17136360, Length=205, Percent_Identity=29.2682926829268, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130249, Length=157, Percent_Identity=35.6687898089172, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24652460, Length=147, Percent_Identity=31.9727891156463, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015891
- InterPro:   IPR020892
- InterPro:   IPR002130 [H]

Pfam domain/function: PF00160 Pro_isomerase [H]

EC number: =5.2.1.8 [H]

Molecular weight: Translated: 20250; Mature: 20250

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: PS00170 CSA_PPIASE_1 ; PS50072 CSA_PPIASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLLALGGAALLATAPAFAQTATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVK
CCCEEEECCCHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
AGQYNGTIFHRVIKGFMIQGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPN
CCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCC
SATAQFFINTVDNGGLDYPNPDGNGYAVFGKVVSGLDVVKKIEGVATTSRGPMQDVPAQP
CCEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VVIESASIVSK
EEEECCHHHCC
>Mature Secondary Structure
MKRLLLALGGAALLATAPAFAQTATAHPVVQLKTSQGDIRVELYPEKAPKSVANFLDYVK
CCCEEEECCCHHHHHCCCHHHHHCCCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHH
AGQYNGTIFHRVIKGFMIQGGGYKTNFEEKPTRAPIPLESRNGLKNLTGTIAMARTSDPN
CCCCCCHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEEEEEECCCCC
SATAQFFINTVDNGGLDYPNPDGNGYAVFGKVVSGLDVVKKIEGVATTSRGPMQDVPAQP
CCEEEEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VVIESASIVSK
EEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9418240 [H]