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The map label for this gene is mls [H]

Identifier: 116690073

GI number: 116690073

Start: 2276613

End: 2278205

Strand: Direct

Name: mls [H]

Synonym: Bcen2424_2052

Alternate gene names: 116690073

Gene position: 2276613-2278205 (Clockwise)

Preceding gene: 116690072

Following gene: 116690075

Centisome position: 65.35

GC content: 65.41

Gene sequence:

>1593_bases
ATGAGCACCCCGATCACGCTGCCGCAAGGCATGACGATCACCGGCGAAATCAAGCCGGGTTACGAAGCCATCCTGACGCC
CGACGCGCTGGCACTCGTCGCGGCGCTGCACCGCACGTTCGAATCGCGCCGCCAGGCGCTGCTGCAGGCGCGCGTCGAGC
GCACGAAACGCCTCGACGCGGGCGAGCGCCCCGATTTCCTGGCCGAGACGAAGGCGATCCGCGAAGGCGACTGGAAGGTC
GCGCCGCTGCCGGCCGACCTGCAATGCCGCCGGGTCGAGATCACGGGGCCCGTCGAGCGCAAGATGATCATCAACGCGCT
GAACTCGGGCGCCGATTCGTACATGACGGACTTCGAGGATTCGAACGCGCCGAGCTGGACGAACCAGATCGACGGCCAGA
TCAACCTGAAGGACGCGGTGCGCCGCACGATCTCGCTCGAGCAGAACGGCAAGTCGTACCGGCTGAACGACAAGGTCGCG
ACGCTGATCGTGCGTCCGCGCGGCTGGCACCTCGACGAGAAGCACGTGACGGTCGACGGCCAGCGCGTGTCCGGCGGCAT
CTTCGATTTCGCGCTGTTCCTGTTCCACAACGCGAAGGAACTGCTCGCGCGCGGCTCGGGCCCGTACTTCTACCTGCCGA
AGATGGAAAGCCATCTCGAGGCGCGCCTGTGGAACGACATCTTCGTCGCCGCGCAGGAAGCGGTCGGCGTGCCGCGCGGC
ACGATCCGCGCGACGGTGCTGATCGAAACGATCCTCGCCGCGTTCGAGATGGACGAGATCCTGTACGAACTGCGCGAACA
CAGCTCGGGCCTGAACGCCGGCCGCTGGGACTACATCTTCTCGGCGATCAAGAAGTTCAAGAACGACCGCGACTTCTGCC
TGGCCGACCGTTCGAAGATCACGATGACGGTGCCGTTCATGCGTGCGTACGCACTGCTGCTGCTGAAGACCTGCCACAAG
CGCAACGCGCCGGCGATCGGCGGGATGAGCGCGCTGATTCCGATCAAGAACGATCCGGAAGCGAACGACAAGGCGATGGG
CGGCGTGCGCTCGGACAAGCAGCGCGACGCGACCGACGGCTACGACGGCGGCTGGGTCGCGCACCCGGGCCTCGTGCCGA
TCGCGATGGAAGAGTTCGTCAAGGTACTCCGCGACAAGCCGAACCAGATCGCGAAGCAGCGCGACGACGTGCAGGTCGAA
GGCAGGAACCTGCTCGACTTCCAGCCGGAAGCGCCGATCACCGAAGCGGGCCTGCGCAACAACATCAACGTCGGGATCCA
CTATCTCGGCGCGTGGCTCGACGGCAACGGCTGCGTGCCGATCCACAACCTGATGGAAGATGCGGCGACGGCCGAGATTT
CCCGCTCGCAGGTGTGGCAATGGATCCGCTCGCCGAAGGGCGTGCTCGACGACGGCCGCAAGGTCACGGCCGAACTCGTG
CGCGAATTCGCGAAGATCGAACTGGACAACGTGAAGCGCGCGGTCGGCGGCAACACGCAACCGTACGAGCGCGCCGCGGC
GATCTTCGAGCAGATGTCGACGTCGGAAGGCTTCACCGAATTCCTGACGCTGCCGCTGTACGAGGAAATCTGA

Upstream 100 bases:

>100_bases
CCGCAAACCGCACGCGCCCCGGCCCGGGTGCATGACGCTCGCAGCTGCCGCCTTCCCCCTTCACCGAAACCGCAACTGAC
CGACCAAGGAGATGAGACTC

Downstream 100 bases:

>100_bases
CGAGCTGTCCGCTGCGATCGTCGGCCGGCTCCACGTCGTGCTGCCGGTCGCGTGCGACGACGGATCAATGGCATGAAAAA
GCGCCCCGCGGGGCGCTTTT

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSTPITLPQGMTITGEIKPGYEAILTPDALALVAALHRTFESRRQALLQARVERTKRLDAGERPDFLAETKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLRDKPNQIAKQRDDVQVE
GRNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
REFAKIELDNVKRAVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Sequences:

>Translated_530_residues
MSTPITLPQGMTITGEIKPGYEAILTPDALALVAALHRTFESRRQALLQARVERTKRLDAGERPDFLAETKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLRDKPNQIAKQRDDVQVE
GRNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
REFAKIELDNVKRAVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
>Mature_529_residues
STPITLPQGMTITGEIKPGYEAILTPDALALVAALHRTFESRRQALLQARVERTKRLDAGERPDFLAETKAIREGDWKVA
PLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVAT
LIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRGT
IRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKITMTVPFMRAYALLLLKTCHKR
NAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLRDKPNQIAKQRDDVQVEG
RNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELVR
EFAKIELDNVKRAVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=513, Percent_Identity=50.2923976608187, Blast_Score=501, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=51.1450381679389, Blast_Score=523, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=53.3333333333333, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=52.5291828793774, Blast_Score=540, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=51.3618677042802, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59288; Mature: 59157

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPITLPQGMTITGEIKPGYEAILTPDALALVAALHRTFESRRQALLQARVERTKRLDA
CCCCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GERPDFLAETKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDG
EEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLRDKPNQIAKQRDDVQVEGRNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEECCCCEECCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
REFAKIELDNVKRAVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
>Mature Secondary Structure 
STPITLPQGMTITGEIKPGYEAILTPDALALVAALHRTFESRRQALLQARVERTKRLDA
CCCCCCCCCCEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GERPDFLAETKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYRLNDKVATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEAVGVPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLADRSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDG
EEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLRDKPNQIAKQRDDVQVEGRNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHHCCCHHHHHCCCCEEECCCCEECCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
REFAKIELDNVKRAVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA