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The map label for this gene is dapE [H]

Identifier: 116690053

GI number: 116690053

Start: 2254911

End: 2256050

Strand: Direct

Name: dapE [H]

Synonym: Bcen2424_2032

Alternate gene names: 116690053

Gene position: 2254911-2256050 (Clockwise)

Preceding gene: 116690052

Following gene: 116690054

Centisome position: 64.72

GC content: 68.68

Gene sequence:

>1140_bases
ATGTCCGCCACCCTAGCCCTTACCGAACAGCTGATCGCCCGCGCGTCCGTGACGCCCGACGACCAGCACTGCCAGCAGAT
CATGACCGAGCGCCTCGCCGCGCTCGGCTTCGAATGCGAGACCATCGCGTCGCACGGCGTGACCAACCTGTGGGCCGTCA
AGCGCGGCACCGACGGCCGCGACGGCAAGCTGCTCGCGTTCGCGGGCCATACCGACGTCGTGCCGACCGGCCCGCTCGAA
CAGTGGACCTCGCCGCCGTTCGTCCCCGCCCATCGCGACGGCAAGCTGTACGGCCGCGGCGCGGCCGACATGAAGACGTC
GCTCGCGGCGTTCGTCGTCGCCTCCGAGGAATTCGTCGCGGCGCATCCGGACCACCGCGGCGCGATCGCGTTCCTGATCA
CGAGCGACGAGGAAGGCCCGGCCACCGACGGCACCGTGAAGGTCGTCGAGCTGCTGCAGGAACGCGGCGAGCGGCTCGAC
TACTGCATCGTCGGCGAGCCGACGTCGACCGCCGAGCTCGGCGACGTCGTCAAGAACGGCCGCCGCGGGTCGATGTCCGG
CGAACTGGTCATCAAGGGCGTGCAAGGCCACATTGCCTATCCGCACCTCGCGAAGAACCCGATCCACCTGCTCGCGCCGG
CGCTCGCCGAGCTCGCCGCCGAGCAATGGGACGCCGGCAACGAATACTTCCCGCCGACCACCTGGCAGGTGTCGAACCTG
CACGCTGGCACCGGCGCGACCAACGTGATCCCGGGCCACGCCGACCTGCTGTTCAACTTCCGCTTCTCGACCGCGAGCAC
CGTCGAAGGCCTGCAGGCCCGCGTGCACGCGATCCTCGACAAGCACGGCCTCGAATACACGCTGAAGTGGTCGGTGAGCG
GCCTGCCGTTCCTCACGCCGCGCGGTGAACTGTCGGGCGCGCTGGAACACGCGATCCGCACCGAGACCGGTATCACGACC
GAGCTGTCGACGACGGGCGGCACGTCGGACGGCCGCTTCATTGCGCGCATCTGCCCGCAGGTGATCGAGTTCGGCCCGCC
GAACGGCAGCATCCACAAGATCGACGAGCACATCGAAGTGCGCTTCGTCGACCCGCTGAAGAACGTGTACCGACGCGCGC
TCGAACAACTGATCGCCTGA

Upstream 100 bases:

>100_bases
TGCCGCATAGGGTCGCCCGCGCTGCGCCCACGGGCGCCGCCCGACGCCCTGTCGCTGCCGGCCACCGCGCCGGCATTTTT
TATCGAAAGTGGTCCGAACC

Downstream 100 bases:

>100_bases
CGGAGCCTCGCATGACGACACCTTTTGCCACAGTCCGCGACCTGCTGCGCTACGCGGTCACGCGCTTCTCGAAAGCGAAG
CTCGCGTTCGGTCACGGTTC

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MSATLALTEQLIARASVTPDDQHCQQIMTERLAALGFECETIASHGVTNLWAVKRGTDGRDGKLLAFAGHTDVVPTGPLE
QWTSPPFVPAHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPDHRGAIAFLITSDEEGPATDGTVKVVELLQERGERLD
YCIVGEPTSTAELGDVVKNGRRGSMSGELVIKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
HAGTGATNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTPRGELSGALEHAIRTETGITT
ELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEVRFVDPLKNVYRRALEQLIA

Sequences:

>Translated_379_residues
MSATLALTEQLIARASVTPDDQHCQQIMTERLAALGFECETIASHGVTNLWAVKRGTDGRDGKLLAFAGHTDVVPTGPLE
QWTSPPFVPAHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPDHRGAIAFLITSDEEGPATDGTVKVVELLQERGERLD
YCIVGEPTSTAELGDVVKNGRRGSMSGELVIKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
HAGTGATNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTPRGELSGALEHAIRTETGITT
ELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEVRFVDPLKNVYRRALEQLIA
>Mature_378_residues
SATLALTEQLIARASVTPDDQHCQQIMTERLAALGFECETIASHGVTNLWAVKRGTDGRDGKLLAFAGHTDVVPTGPLEQ
WTSPPFVPAHRDGKLYGRGAADMKTSLAAFVVASEEFVAAHPDHRGAIAFLITSDEEGPATDGTVKVVELLQERGERLDY
CIVGEPTSTAELGDVVKNGRRGSMSGELVIKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNLH
AGTGATNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTPRGELSGALEHAIRTETGITTE
LSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEVRFVDPLKNVYRRALEQLIA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788816, Length=376, Percent_Identity=56.9148936170213, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1790395, Length=389, Percent_Identity=24.6786632390745, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789236, Length=269, Percent_Identity=22.3048327137546, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17551016, Length=277, Percent_Identity=24.9097472924188, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.5.1.18 [H]

Molecular weight: Translated: 40858; Mature: 40726

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATLALTEQLIARASVTPDDQHCQQIMTERLAALGFECETIASHGVTNLWAVKRGTDGR
CCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCC
DGKLLAFAGHTDVVPTGPLEQWTSPPFVPAHRDGKLYGRGAADMKTSLAAFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHEEEECCHHEE
AHPDHRGAIAFLITSDEEGPATDGTVKVVELLQERGERLDYCIVGEPTSTAELGDVVKNG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCC
RRGSMSGELVIKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
CCCCCCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCE
HAGTGATNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTP
ECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEECC
RGELSGALEHAIRTETGITTELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEV
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEE
RFVDPLKNVYRRALEQLIA
EEECHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SATLALTEQLIARASVTPDDQHCQQIMTERLAALGFECETIASHGVTNLWAVKRGTDGR
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEEECCCCCC
DGKLLAFAGHTDVVPTGPLEQWTSPPFVPAHRDGKLYGRGAADMKTSLAAFVVASEEFVA
CCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCCCEEECCCHHHHHHHHHEEEECCHHEE
AHPDHRGAIAFLITSDEEGPATDGTVKVVELLQERGERLDYCIVGEPTSTAELGDVVKNG
ECCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCC
RRGSMSGELVIKGVQGHIAYPHLAKNPIHLLAPALAELAAEQWDAGNEYFPPTTWQVSNL
CCCCCCCEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCE
HAGTGATNVIPGHADLLFNFRFSTASTVEGLQARVHAILDKHGLEYTLKWSVSGLPFLTP
ECCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEECC
RGELSGALEHAIRTETGITTELSTTGGTSDGRFIARICPQVIEFGPPNGSIHKIDEHIEV
CHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCEEEECCCEEE
RFVDPLKNVYRRALEQLIA
EEECHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA