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The map label for this gene is ligA [H]

Identifier: 116690045

GI number: 116690045

Start: 2242818

End: 2244893

Strand: Reverse

Name: ligA [H]

Synonym: Bcen2424_2024

Alternate gene names: 116690045

Gene position: 2244893-2242818 (Counterclockwise)

Preceding gene: 116690046

Following gene: 116690044

Centisome position: 64.44

GC content: 68.21

Gene sequence:

>2076_bases
ATGGCCCGAACCCAAGCCGAACCGCCAGCCAGCCAGCCCGACGCGCGCGCCGCGTGGCTGCGCGACCAACTCGAGCGGGC
GAACTACGCCTACTACGTGCTCGACCAGCCGGATCTGCCCGACGCCGAATACGACCGGCTGTTCCGCGAGTTGCAGCAGC
TCGAAACCGACCATCCCGAACTCGTGACACCCGATTCGCCGACGCAGCGTGTCGGCGGCGAAGCGGCCGGCGGCTTCACG
CCGGTCGTCCACGATGCGCCGATGCTGTCGCTGAACAACGGCTTTGCCGACGAGGACATCGTCGCGTTCGACAAGCGCGT
CGCCGACGCGCTCGCCAAGACGACCGATCTGGCCGGCTCGGTGACCGACCCCGTCGAATACGCGTGCGAACTGAAGTTCG
ACGGCCTGGCGATCTCGCTGCGCTACGAGCAAGGCGTGTTCGTGCAGGCCGCGACGCGTGGCGACGGGACGACGGGCGAG
GACGTGACCGAGAACGTGCGCACGATCCGCTCGATTCCGCTGAAGCTGAAGGGCAAGCACGTGCCGGCGGTGCTCGACGT
ACGCGGCGAGGTGCTGATGTTCAAGCGCGATTTCGCACGCCTGAACGAACGCCAGCGCGCGGCCGAGCAGCGCGAATTCG
CGAACCCGCGCAACGCAGCGGCCGGCAGCCTGCGCCAGCTCGACTCGAAGATCACCGCGCAGCGGCCGCTGTCGTTCTTC
GCGTACGGGATCGGCGTGCTCGACGGGATGCCGATGCCCGACACGCACAGCGCGCTGCTCGACTGGTACGAGTCGCTCGG
CCTGCCGGTGAATCGGGAGCGCGCGGTCGTGCACGGCGCCGAAGGCTTGCTCGACTTCTTCCGCAAGGTCGGCGAGAAGC
GCGAGTCGCTGCCGTACGACATCGACGGCGTCGTCTACAAGGTCAACCGGCGCGACGAGCAGGAGCGGCTCGGCTTCGTG
TCGCGTGCGCCGCGCTTCGCGCTCGCGCACAAGTTCCCCGCGCAGGAAGCGCTGACGAAGCTCGTCGCGATCGACGTGCA
GGTCGGCCGCACCGGTGCGATCACGCCGGTCGCGCGTCTCGAGCCGGTGTTCGTCGGCGGCGCGACCGTGACCAATGCGA
CGCTGCACAACGAGGACGAAGTGCGCCGCAAGGACATCCGGATCGGCGACACCGTGATCGTGCGGCGTGCGGGCGACGTG
ATTCCGGAAGTCGTCGGCGCGCTGCTCGACCGGCGCCCGGCGGATGCGGCCGAATTCGTGATGCCGACCGAATGCCCGGT
GTGCGGCTCGAAGATCGAGCGCCTGCCGGACGAGGCGATCGCGCGCTGCACGGGCGGGCTGTTCTGCCCGGCGCAGCGCA
AGCAGGCGCTGTGGCATTTCGCGCAACGCCGCGCGCTCGACATCGACGGCCTCGGCGAGAAGATCATCGACCAGCTCGTC
GAGCTGAATCTCGTGCGCACGCCGGCCGACCTGTTCAATCTGGGCTTCGCGACGCTGGCCGAACTCGACCGGTTCGCCGA
GAAGTCCGCGCAGAACCTGATCGATTCGCTCGAGAAGGCCAAGCATACGACGCTCGCGCGCTTCATCTACGGGCTCGGCA
TCCGCCATGTCGGCGAATCGACCGCGAAGGATCTCGCGAAGCATTTCGGCTCGCTGACGCCGATCATGGACGCGTCGATC
GAGGAACTGCTCGAAGTCAACGACGTCGGGCCGATCGTGGCCGAGTCGCTCCACCAGTTCTTCGCGGAGGAGCACAACCG
GACCGTGATCGAGCAGTTGCGCGCGCCGGGCAAGGTCACGTGGCCCGAAGGGCCGCCCGCGCCGAAGGCGCCGCAGGGCG
TGCTGGCCGGCAAGACGGTCGTGCTGACGGGCACGCTGCCGAATCTCACGCGCGATGCGGCGAAGGAGATGCTCGAAGCG
GCGGGCGCGAAAGTGGCCGGTTCGGTGTCGAAGAAGACGGATTACGTGGTCGCGGGCGCCGAAGCCGGCAGCAAGCTCGC
GAAGGCCGAGGAACTCGGCATCCCCGTGCTGGACGAAGATGGTCTGCACCAACTCCTGGAGGGCAATACGCCATGA

Upstream 100 bases:

>100_bases
TGCGCTGCAGCACGTCATCCGGCCCGCATTGCGTACCCCGCCGCGGCGTCAAACTGAGATAATCGGGCATCCGATTTTTC
AGAACGACTCTGCCGCCAGC

Downstream 100 bases:

>100_bases
TTCGCGAGATCCTGAAGATGGGCGATCCGCGCCTGCTCGAAATCGCCCAGCCGGTCGAGCGGTTCGATACACCCGAGCTG
CACGAGATCGTCGCGGACAT

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPELVTPDSPTQRVGGEAAGGFT
PVVHDAPMLSLNNGFADEDIVAFDKRVADALAKTTDLAGSVTDPVEYACELKFDGLAISLRYEQGVFVQAATRGDGTTGE
DVTENVRTIRSIPLKLKGKHVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
AYGIGVLDGMPMPDTHSALLDWYESLGLPVNRERAVVHGAEGLLDFFRKVGEKRESLPYDIDGVVYKVNRRDEQERLGFV
SRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVGALLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYGLGIRHVGESTAKDLAKHFGSLTPIMDASI
EELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDAAKEMLEA
AGAKVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGLHQLLEGNTP

Sequences:

>Translated_691_residues
MARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPELVTPDSPTQRVGGEAAGGFT
PVVHDAPMLSLNNGFADEDIVAFDKRVADALAKTTDLAGSVTDPVEYACELKFDGLAISLRYEQGVFVQAATRGDGTTGE
DVTENVRTIRSIPLKLKGKHVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
AYGIGVLDGMPMPDTHSALLDWYESLGLPVNRERAVVHGAEGLLDFFRKVGEKRESLPYDIDGVVYKVNRRDEQERLGFV
SRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVGALLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYGLGIRHVGESTAKDLAKHFGSLTPIMDASI
EELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDAAKEMLEA
AGAKVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGLHQLLEGNTP
>Mature_690_residues
ARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPELVTPDSPTQRVGGEAAGGFTP
VVHDAPMLSLNNGFADEDIVAFDKRVADALAKTTDLAGSVTDPVEYACELKFDGLAISLRYEQGVFVQAATRGDGTTGED
VTENVRTIRSIPLKLKGKHVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFFA
YGIGVLDGMPMPDTHSALLDWYESLGLPVNRERAVVHGAEGLLDFFRKVGEKRESLPYDIDGVVYKVNRRDEQERLGFVS
RAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVI
PEVVGALLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVE
LNLVRTPADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYGLGIRHVGESTAKDLAKHFGSLTPIMDASIE
ELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDAAKEMLEAA
GAKVAGSVSKKTDYVVAGAEAGSKLAKAEELGIPVLDEDGLHQLLEGNTP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=46.1538461538462, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=58.5949177877429, Blast_Score=766, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=418, Percent_Identity=25.8373205741627, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324791, Length=90, Percent_Identity=43.3333333333333, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI62472391, Length=92, Percent_Identity=44.5652173913043, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737765, Length=92, Percent_Identity=44.5652173913043, Blast_Score=68, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75478; Mature: 75347

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
LVTPDSPTQRVGGEAAGGFTPVVHDAPMLSLNNGFADEDIVAFDKRVADALAKTTDLAGS
EECCCCCHHHCCCCCCCCCCCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
VTDPVEYACELKFDGLAISLRYEQGVFVQAATRGDGTTGEDVTENVRTIRSIPLKLKGKH
CCCHHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCC
VPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
CCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
AYGIGVLDGMPMPDTHSALLDWYESLGLPVNRERAVVHGAEGLLDFFRKVGEKRESLPYD
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQERLGFVSRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARL
CCCEEEEECCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALLDRRPADAAEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYGLGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLTPIMDASIEELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVT
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
WPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
EAGSKLAKAEELGIPVLDEDGLHQLLEGNTP
CCCHHHHHHHHCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
ARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPE
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
LVTPDSPTQRVGGEAAGGFTPVVHDAPMLSLNNGFADEDIVAFDKRVADALAKTTDLAGS
EECCCCCHHHCCCCCCCCCCCHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
VTDPVEYACELKFDGLAISLRYEQGVFVQAATRGDGTTGEDVTENVRTIRSIPLKLKGKH
CCCHHHHHEEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCC
VPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
CCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
AYGIGVLDGMPMPDTHSALLDWYESLGLPVNRERAVVHGAEGLLDFFRKVGEKRESLPYD
HHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQERLGFVSRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARL
CCCEEEEECCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALLDRRPADAAEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLIDSLEKAKHTTLARFIYGLGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLTPIMDASIEELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVT
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
WPEGPPAPKAPQGVLAGKTVVLTGTLPNLTRDAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
EAGSKLAKAEELGIPVLDEDGLHQLLEGNTP
CCCHHHHHHHHCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA