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The map label for this gene is pncA [H]

Identifier: 116689926

GI number: 116689926

Start: 2111800

End: 2112432

Strand: Reverse

Name: pncA [H]

Synonym: Bcen2424_1905

Alternate gene names: 116689926

Gene position: 2112432-2111800 (Counterclockwise)

Preceding gene: 116689927

Following gene: 116689925

Centisome position: 60.63

GC content: 70.77

Gene sequence:

>633_bases
ATGAAACGCACCGACGACGTCCTGCTCGTGATCGACGTGCAATACGACTTCATGCCGGGCGGCGCGCTCGCGGTGCCCGA
CGGCGATGCGGTCGTGCCGGTGATCAACGCGCTCGCGCAATGCTTCGACCAGGTCGTGCTCACGCAGGACTGGCACCCGC
GCGAGCACGTGTCGTTCGCGGCGAACCATCCGGGCCGCGAGCCGTTCTCGACGCTCGCGCTGCCGTACGGCGAGCAGGTG
CTGTGGCCCGTGCACTGCGTGCAGGACACCGACGGCGCGGCGCTGCATCGCGACCTCGACATCCCGCACGCGCGGCTCGT
GATCCGCAAGGGCGGCGACGCGCAGGTCGACAGCTATTCCGCGTTCGTCGAAGCCGACCGCACGACACGCACGGGGCTCG
CGGGCTATCTGCGCGAACTCGGGGCGAAGCGCGTGTGGTGCTGCGGGCTCGCGACCGACTATTGCGTCGCGTGGTCGGCG
CTCGATGCGCGCGCGGCCGGTTTCGAAGCCGCGGTGATCAACGATGCGTGCCGTGCGATCGACCTGAACGGGTCGCTCGC
GCACGCATGGCAGCAGATGCAGGCAGCGGGCGTCGTGCATGCGACGTCCGCGGGCGCGCGCCCGGCGGCGTAG

Upstream 100 bases:

>100_bases
GCGCCGTCGATCGCGGAACTGGCGAAGGCCGGCCGCGGGTTCAACGGCTGACGCCGCCGCATCGCATCGCGGCGCGACCG
ATCCTGTCTGGAGACACGCA

Downstream 100 bases:

>100_bases
CGCATTTCCCATCCGAATCAGCAGGACCGAAGGAGACTCGCATGACCGAAGCCGTAATCGTATCGACCGCACGCACGCCG
CTGGCGAAATCCTGGCGCGG

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQCFDQVVLTQDWHPREHVSFAANHPGREPFSTLALPYGEQV
LWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYSAFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVINDACRAIDLNGSLAHAWQQMQAAGVVHATSAGARPAA

Sequences:

>Translated_210_residues
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQCFDQVVLTQDWHPREHVSFAANHPGREPFSTLALPYGEQV
LWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYSAFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVINDACRAIDLNGSLAHAWQQMQAAGVVHATSAGARPAA
>Mature_210_residues
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQCFDQVVLTQDWHPREHVSFAANHPGREPFSTLALPYGEQV
LWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYSAFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVINDACRAIDLNGSLAHAWQQMQAAGVVHATSAGARPAA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=202, Percent_Identity=43.5643564356436, Blast_Score=149, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI193206735, Length=224, Percent_Identity=33.0357142857143, Blast_Score=100, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17543130, Length=224, Percent_Identity=33.4821428571429, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI71999339, Length=215, Percent_Identity=33.0232558139535, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=189, Percent_Identity=34.9206349206349, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648247, Length=229, Percent_Identity=34.061135371179, Blast_Score=125, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22561; Mature: 22561

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQCFDQVVLTQDWHPREHVSFA
CCCCCCEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCEEE
ANHPGREPFSTLALPYGEQVLWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYS
CCCCCCCCHHHEECCCCCCEEEEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCHH
AFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSALDARAAGFEAAVINDACRAI
HEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHEECCCHHEE
DLNGSLAHAWQQMQAAGVVHATSAGARPAA
ECCCHHHHHHHHHHHCCEEEECCCCCCCCC
>Mature Secondary Structure
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQCFDQVVLTQDWHPREHVSFA
CCCCCCEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCEEE
ANHPGREPFSTLALPYGEQVLWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYS
CCCCCCCCHHHEECCCCCCEEEEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCHH
AFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSALDARAAGFEAAVINDACRAI
HEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHEECCCHHEE
DLNGSLAHAWQQMQAAGVVHATSAGARPAA
ECCCHHHHHHHHHHHCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]