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The map label for this gene is acoB [H]

Identifier: 116689859

GI number: 116689859

Start: 2042055

End: 2043059

Strand: Reverse

Name: acoB [H]

Synonym: Bcen2424_1838

Alternate gene names: 116689859

Gene position: 2043059-2042055 (Counterclockwise)

Preceding gene: 116689860

Following gene: 116689858

Centisome position: 58.64

GC content: 67.36

Gene sequence:

>1005_bases
ATGGCAAGGAAGATCACTTATTCGCAGGCGATCAACGAGGCGCTCGCGCAGGAAATGGCGCGCGACGACAGCGTGATCGT
GATGGGCGAGGACAACGCGGGCGGTGCGGGCGCGCCGGGCGAGGACGACGCATGGGGCGGCGTGCTCGGCGTGACGAAGG
GGCTGTTCCACAAGTTTCCGGGCCGCGTGCTCGATACGCCGCTGTCGGAGGGCGGCTACATCGGCGCGGCGGTCGGCGCC
GCCGCGTGCGGGATGCGTCCCGTCGCGGAACTGATGTTCATCGATTTCATGGGCGTGTGTTTCGACCAGATCTTCAACCA
GGCCGCGAAATTCCGCTACATGTTCGGCGGCAAGGCCGTGACGCCGGTCGTGATCCGCGCGATGTACGGCGCCGGCCTGC
GCGCGGCCGCGCAGCACTCGCAGATGCTCACGTCGCTGTTCACGCATATCCCGGGGCTGAAGGTCGTGTGCCCGTCGACG
CCGTACGACGCGAAGGGGCTGCTGATCCAGGCGATCCGCGACAACGATCCCGTGATCTTCCTCGAACACAAGCTGCTCTA
TACGCGTGAGGGCGACGTCCCGGAGGAATCCTATGCGATTCCGTTCGGCGAGGCGAACGTGCTGCGCGACGGCGACGACG
CGACGATCGTCACGTACGGCCGGATGGTGCATCTCGCGATGGACGCGGCCGCGACGCTCGCGAAGGGCGGCATCCAGTGC
GACGTGATCGACCTGCGCACGACGTCGCCGCTCGACGAGGAAACGATCCTCGAAAGCGCGGCGCGCACCGGGCGCGTGGT
GGTGGTCGACGAAGCGAATCCGCGCTGCTCGATCGCGACCGACATCGCCGCGCTCGTCGCGCAGCGCGCGTTCCGGTCTC
TGAAGGCGCCGATCGAGCTCGTCACCGCGCCGCATACGCCCGCGCCGTTCGCCGGCGTGCTGGAAGACCTGTATATCCCG
TCCGCCGATGCAATTGCGCAGGCGGTACTCAAGACGAGGAGCTGA

Upstream 100 bases:

>100_bases
GGATGCCGCCGACCTGCTGGCCGACGTCTACGTGTCGTATCCGTGAGCGCGGCCTGATCCAGCAGCCGGCACGGCGAACC
GAACATTCCAGGAGACAGAC

Downstream 100 bases:

>100_bases
CACGATGTCGATTCACATGATCACGATGCCCAAATGGGGGCTGTCGATGGAGCAGGGGCAGGTCAACGGCTGGCTGAAGG
CGGTCGGCGAACGCGTGGCG

Product: transketolase, central region

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFPGRVLDTPLSEGGYIGAAVGA
AACGMRPVAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAVTPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPST
PYDAKGLLIQAIRDNDPVIFLEHKLLYTREGDVPEESYAIPFGEANVLRDGDDATIVTYGRMVHLAMDAAATLAKGGIQC
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLKAPIELVTAPHTPAPFAGVLEDLYIP
SADAIAQAVLKTRS

Sequences:

>Translated_334_residues
MARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFPGRVLDTPLSEGGYIGAAVGA
AACGMRPVAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAVTPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPST
PYDAKGLLIQAIRDNDPVIFLEHKLLYTREGDVPEESYAIPFGEANVLRDGDDATIVTYGRMVHLAMDAAATLAKGGIQC
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLKAPIELVTAPHTPAPFAGVLEDLYIP
SADAIAQAVLKTRS
>Mature_333_residues
ARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFPGRVLDTPLSEGGYIGAAVGAA
ACGMRPVAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAVTPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPSTP
YDAKGLLIQAIRDNDPVIFLEHKLLYTREGDVPEESYAIPFGEANVLRDGDDATIVTYGRMVHLAMDAAATLAKGGIQCD
VIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLKAPIELVTAPHTPAPFAGVLEDLYIPS
ADAIAQAVLKTRS

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The beta subunit is probably not the catalytic subunit of the enzyme [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=337, Percent_Identity=40.0593471810089, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI291084858, Length=337, Percent_Identity=38.2789317507418, Blast_Score=224, Evalue=7e-59,
Organism=Homo sapiens, GI4557353, Length=336, Percent_Identity=37.797619047619, Blast_Score=218, Evalue=6e-57,
Organism=Homo sapiens, GI34101272, Length=336, Percent_Identity=37.797619047619, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI17538422, Length=337, Percent_Identity=40.3560830860534, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17506935, Length=334, Percent_Identity=40.1197604790419, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319698, Length=339, Percent_Identity=38.6430678466077, Blast_Score=232, Evalue=5e-62,
Organism=Drosophila melanogaster, GI21358145, Length=336, Percent_Identity=39.2857142857143, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24650940, Length=336, Percent_Identity=39.2857142857143, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI160714832, Length=332, Percent_Identity=35.8433734939759, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI160714828, Length=332, Percent_Identity=35.8433734939759, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI24650943, Length=103, Percent_Identity=39.8058252427184, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650945, Length=103, Percent_Identity=39.8058252427184, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35501; Mature: 35370

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFP
CCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GRVLDTPLSEGGYIGAAVGAAACGMRPVAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAV
CHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCH
TPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPSTPYDAKGLLIQAIRDNDPVIF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEEE
LEHKLLYTREGDVPEESYAIPFGEANVLRDGDDATIVTYGRMVHLAMDAAATLAKGGIQC
EEEEEEEEECCCCCCCCEECCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCEE
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLKAPIEL
EEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
VTAPHTPAPFAGVLEDLYIPSADAIAQAVLKTRS
EECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARKITYSQAINEALAQEMARDDSVIVMGEDNAGGAGAPGEDDAWGGVLGVTKGLFHKFP
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
GRVLDTPLSEGGYIGAAVGAAACGMRPVAELMFIDFMGVCFDQIFNQAAKFRYMFGGKAV
CHHHCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCCH
TPVVIRAMYGAGLRAAAQHSQMLTSLFTHIPGLKVVCPSTPYDAKGLLIQAIRDNDPVIF
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCEEE
LEHKLLYTREGDVPEESYAIPFGEANVLRDGDDATIVTYGRMVHLAMDAAATLAKGGIQC
EEEEEEEEECCCCCCCCEECCCCCCCCEECCCCCEEEEEHHHHHHHHHHHHHHHCCCCEE
DVIDLRTTSPLDEETILESAARTGRVVVVDEANPRCSIATDIAALVAQRAFRSLKAPIEL
EEEECCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEE
VTAPHTPAPFAGVLEDLYIPSADAIAQAVLKTRS
EECCCCCCCHHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]