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The map label for this gene is nfdA [H]

Identifier: 116689418

GI number: 116689418

Start: 1539483

End: 1541372

Strand: Direct

Name: nfdA [H]

Synonym: Bcen2424_1396

Alternate gene names: 116689418

Gene position: 1539483-1541372 (Clockwise)

Preceding gene: 116689417

Following gene: 116689419

Centisome position: 44.19

GC content: 67.51

Gene sequence:

>1890_bases
ATGAGCGCAACCGATACCCCACCCGATCTCATCCTGCACAACGGGCGCATCACGACGCTCGATCGTGCGAACCCGGTCGC
GACGGCGGTCGCGATTGCCGCGGGACGCTTCGTCGCCGTCGGCAGCGAGGCCGACGTGATGCCGCTCGCGGGCCGCGCGA
CGAAGGTGGTCGATCTCGGCGGCCGCGGCGTGCTGCCGGGTCTGATCGACAACCACTGCCACGTGATTCGTGGCGGGCTG
AACTACAACATGGAACTGCGCTGGGACGGCGTGCCGTCGCTGGCGCTCGCGATGGAGATGCTCAAGCAGCAGGTCGCGAT
CACGCCCGCGCCGCAGTGGGTGCGCGTGGTCGGCGGCTTCACCGAACATCAGTTCGTGGAGAAGCGGCTGCCGACGATCG
AAGAACTGAACGCAGTCGCGCCCGATACGCCGGTATTCATCCTGCACCTGTACGATCGCGCGCTGCTGAACGCGGCCGCG
CTGCGGGTGGTCGGCTATACGAAGGACACGCCGGAGCCGCCGGGCGGCACGATCCTGCGCGATGCCGCGGGCAACCCGAC
CGGCCTGCTGCTCGCGAACCCGAACGCGACGATCCTCTACGCGACGCTCGCGAAAGGCCCGAAGCTGCCGTTCGAGTATC
AGTACAACTCGACGCGCCATTTCATGCGCGAGCTGAACCGGCTCGGCGTGACGGGCGTGATCGACGCGGGCGGCGGCTCG
CAGAATTATCCCGACGATTACGAGGTGATCCGCAAGCTGCACGATGCGGGCGAGATGACGATCCGGATCGCGTACAACCT
GTTCACGCAGAAGCCGAACGCGGAGAAGGAAGACTTCGTGAACTGGACGAAGAGCGTCAAGTACCACGATGGCACCGACT
ACTTCCGCAACAACGGCGCGGGCGAGATGCTGGCGTTTTCCGCGGCCGACTTCGAGGACTTCCGCGTCGCGCGGCCGGAC
CTGCCCGCGCAGATGGAGGACGACCTCGAAGGCGTCGTGCGCGTGCTCGCGGAAAACCGTTGGCCGTGGCGCATGCACGC
GACCTATGACGAAACAATCAGCCGCGCGCTCGACGTGTTCGAGAAGGTCAACGAGGACATCCCGCTCGCAGGGCTGAACT
GGTTCTTCGATCACGCGGAAACGATCACCGAGCAATCGATGGACCGGATCGCCGCGCTCGGCGGCGGTATCGCGGTGCAG
CACCGGATGGCGTACCAGGGCGAGTACTTCGTCGAGCGCTACGGCGCGCAAGCCGCGGAGGCGACGCCGCCGGTCGCGAA
GATGCTCGCCAAGGGGCTCAAGGTGTCGGCCGGCACGGACGCGACGCGCGTCGCGTCGTACAACCCGTGGGTGTCGCTTG
CATGGCTCGTGACGGGCAAGACGGTCGGCGGCCTGAGGATGTATCCGCAGCGCAACCTGCTTGATCGCGAAACCGCGCTG
CGGATGTGGACCGAGTATGTGACGTGGTTCTCGAACGAGGAAGGCAAGAAAGGACGCATCGCGGTCGGGCAGCTTGCGGA
CCTGGTCGTCCCGGATCGCGATTTCTTCGGGTGCGCGGAGGACGATATCGCCGGCACGACCGCGTTGCTGACGGTGGTCG
ACGGGAAGATCGTGTGGGGCGCGGGGCCGTTCGCGTCGCACGACGCGCCGATTCCGCCGGCGATGCCGGACTGGTCGCCG
GTGCGCGAATTCGGCGGCTATGGCGGCTGGGGTGCGACGCAGCGCGACGGCGCGCCGTTGCAGCGCGCGGCGGCCGCCGC
GATGTGCGCCTGCGCGAACGAATGCAACGTTCACCAGCATGCGCACGCGGGCGCGTGGGGCCGCACGTTGCCGACGTCGG
ACGCGAAGGGCTTCTGGGGCGCGTTCGGGTGCTCGTGCTGGGCGGTCTGA

Upstream 100 bases:

>100_bases
CGCTGATCGCGCTGGTCGGCCTCGCCGGCATGCTGGTCGGCGCGCAGGCCATCGCGCCGGTTCGTCACTGGCTCGGCTTC
TGAGCACATCGAGGACATTC

Downstream 100 bases:

>100_bases
CATGACGACGCGCCCCGTCTACGGCACCCCCGCGTGGGTCCGCGTGCTGCTGTCGCAGCCGTGGGTCCTGCCGCTCGCGC
GCCTCGCGCTGGTGTCCGCC

Product: amidohydrolase 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSATDTPPDLILHNGRITTLDRANPVATAVAIAAGRFVAVGSEADVMPLAGRATKVVDLGGRGVLPGLIDNHCHVIRGGL
NYNMELRWDGVPSLALAMEMLKQQVAITPAPQWVRVVGGFTEHQFVEKRLPTIEELNAVAPDTPVFILHLYDRALLNAAA
LRVVGYTKDTPEPPGGTILRDAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
QNYPDDYEVIRKLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRNNGAGEMLAFSAADFEDFRVARPD
LPAQMEDDLEGVVRVLAENRWPWRMHATYDETISRALDVFEKVNEDIPLAGLNWFFDHAETITEQSMDRIAALGGGIAVQ
HRMAYQGEYFVERYGAQAAEATPPVAKMLAKGLKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
RMWTEYVTWFSNEEGKKGRIAVGQLADLVVPDRDFFGCAEDDIAGTTALLTVVDGKIVWGAGPFASHDAPIPPAMPDWSP
VREFGGYGGWGATQRDGAPLQRAAAAAMCACANECNVHQHAHAGAWGRTLPTSDAKGFWGAFGCSCWAV

Sequences:

>Translated_629_residues
MSATDTPPDLILHNGRITTLDRANPVATAVAIAAGRFVAVGSEADVMPLAGRATKVVDLGGRGVLPGLIDNHCHVIRGGL
NYNMELRWDGVPSLALAMEMLKQQVAITPAPQWVRVVGGFTEHQFVEKRLPTIEELNAVAPDTPVFILHLYDRALLNAAA
LRVVGYTKDTPEPPGGTILRDAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
QNYPDDYEVIRKLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRNNGAGEMLAFSAADFEDFRVARPD
LPAQMEDDLEGVVRVLAENRWPWRMHATYDETISRALDVFEKVNEDIPLAGLNWFFDHAETITEQSMDRIAALGGGIAVQ
HRMAYQGEYFVERYGAQAAEATPPVAKMLAKGLKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
RMWTEYVTWFSNEEGKKGRIAVGQLADLVVPDRDFFGCAEDDIAGTTALLTVVDGKIVWGAGPFASHDAPIPPAMPDWSP
VREFGGYGGWGATQRDGAPLQRAAAAAMCACANECNVHQHAHAGAWGRTLPTSDAKGFWGAFGCSCWAV
>Mature_628_residues
SATDTPPDLILHNGRITTLDRANPVATAVAIAAGRFVAVGSEADVMPLAGRATKVVDLGGRGVLPGLIDNHCHVIRGGLN
YNMELRWDGVPSLALAMEMLKQQVAITPAPQWVRVVGGFTEHQFVEKRLPTIEELNAVAPDTPVFILHLYDRALLNAAAL
RVVGYTKDTPEPPGGTILRDAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGSQ
NYPDDYEVIRKLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRNNGAGEMLAFSAADFEDFRVARPDL
PAQMEDDLEGVVRVLAENRWPWRMHATYDETISRALDVFEKVNEDIPLAGLNWFFDHAETITEQSMDRIAALGGGIAVQH
RMAYQGEYFVERYGAQAAEATPPVAKMLAKGLKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETALR
MWTEYVTWFSNEEGKKGRIAVGQLADLVVPDRDFFGCAEDDIAGTTALLTVVDGKIVWGAGPFASHDAPIPPAMPDWSPV
REFGGYGGWGATQRDGAPLQRAAAAAMCACANECNVHQHAHAGAWGRTLPTSDAKGFWGAFGCSCWAV

Specific function: Hydrolyzes N-substituted formamides, but not amides. N- benzylformamide is the preferred substrate, while N-butylformamide is hydrolyzed at a much lower rate. Has very low activity towards allylformamide, N-(2-cyclohex-1-enylethyl)formamide and N-(alpha-

COG id: COG1574

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013108
- InterPro:   IPR011059 [H]

Pfam domain/function: PF07969 Amidohydro_3 [H]

EC number: =3.5.1.91 [H]

Molecular weight: Translated: 68616; Mature: 68485

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATDTPPDLILHNGRITTLDRANPVATAVAIAAGRFVAVGSEADVMPLAGRATKVVDLG
CCCCCCCHHHEEECCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCEECCCCCEEEEECC
GRGVLPGLIDNHCHVIRGGLNYNMELRWDGVPSLALAMEMLKQQVAITPAPQWVRVVGGF
CCCCCCHHHCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
TEHQFVEKRLPTIEELNAVAPDTPVFILHLYDRALLNAAALRVVGYTKDTPEPPGGTILR
HHHHHHHHHCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEE
DAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
CCCCCCCEEEEECCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCEEEEECCCCC
QNYPDDYEVIRKLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRNNGA
CCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCEECCCCHHHHCCCC
GEMLAFSAADFEDFRVARPDLPAQMEDDLEGVVRVLAENRWPWRMHATYDETISRALDVF
CCEEEEECCCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNEDIPLAGLNWFFDHAETITEQSMDRIAALGGGIAVQHRMAYQGEYFVERYGAQAAE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCC
ATPPVAKMLAKGLKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHH
RMWTEYVTWFSNEEGKKGRIAVGQLADLVVPDRDFFGCAEDDIAGTTALLTVVDGKIVWG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEC
AGPFASHDAPIPPAMPDWSPVREFGGYGGWGATQRDGAPLQRAAAAAMCACANECNVHQH
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
AHAGAWGRTLPTSDAKGFWGAFGCSCWAV
CCCCCCCCCCCCCCCCCCCHHHCCEEECC
>Mature Secondary Structure 
SATDTPPDLILHNGRITTLDRANPVATAVAIAAGRFVAVGSEADVMPLAGRATKVVDLG
CCCCCCHHHEEECCEEEEECCCCHHHHHHHHHCCCEEEECCCCCCEECCCCCEEEEECC
GRGVLPGLIDNHCHVIRGGLNYNMELRWDGVPSLALAMEMLKQQVAITPAPQWVRVVGGF
CCCCCCHHHCCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCC
TEHQFVEKRLPTIEELNAVAPDTPVFILHLYDRALLNAAALRVVGYTKDTPEPPGGTILR
HHHHHHHHHCCCHHHHHCCCCCCCEEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCEEE
DAAGNPTGLLLANPNATILYATLAKGPKLPFEYQYNSTRHFMRELNRLGVTGVIDAGGGS
CCCCCCCEEEEECCCCEEEEEEECCCCCCCEEECCCCHHHHHHHHHHCCCEEEEECCCCC
QNYPDDYEVIRKLHDAGEMTIRIAYNLFTQKPNAEKEDFVNWTKSVKYHDGTDYFRNNGA
CCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCEECCCCHHHHCCCC
GEMLAFSAADFEDFRVARPDLPAQMEDDLEGVVRVLAENRWPWRMHATYDETISRALDVF
CCEEEEECCCCCHHEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
EKVNEDIPLAGLNWFFDHAETITEQSMDRIAALGGGIAVQHRMAYQGEYFVERYGAQAAE
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCHHHHHHHCCCCCC
ATPPVAKMLAKGLKVSAGTDATRVASYNPWVSLAWLVTGKTVGGLRMYPQRNLLDRETAL
CCCHHHHHHHCCCEEECCCCCCEEECCCCCEEEEEEEECCCCCCEEECCCCCCHHHHHHH
RMWTEYVTWFSNEEGKKGRIAVGQLADLVVPDRDFFGCAEDDIAGTTALLTVVDGKIVWG
HHHHHHHHHHCCCCCCCCCEEHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEC
AGPFASHDAPIPPAMPDWSPVREFGGYGGWGATQRDGAPLQRAAAAAMCACANECNVHQH
CCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHH
AHAGAWGRTLPTSDAKGFWGAFGCSCWAV
CCCCCCCCCCCCCCCCCCCHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA