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The map label for this gene is amtB [C]

Identifier: 116689224

GI number: 116689224

Start: 1326243

End: 1327436

Strand: Reverse

Name: amtB [C]

Synonym: Bcen2424_1202

Alternate gene names: 116689224

Gene position: 1327436-1326243 (Counterclockwise)

Preceding gene: 116689225

Following gene: 116689223

Centisome position: 38.1

GC content: 69.1

Gene sequence:

>1194_bases
ATGGATGGTCTGAAATCCGGCGTCGACACACTGTTCCTGTTGATCGGCGCCGTCATGGTGCTCGCGATGCACGCGGGCTT
CGCGTTTCTCGAACTCGGCACGGTCCGCAAGAAGAACCAGGTCAACGCGCTCGTGAAGATCCTGGTCGACTTCTCGGTGT
CGACGCTCGCGTACTTCTTCATCGGCTACACGATCGCGTACGGCGTCGAATTCTTCGACGACATCGGCACGCTGTCGCAG
CACAACGGCTACGCGCTCGTCCGCTTCTTTTTCCTGCTGACGTTCGCGGCGGCGATCCCGGCGATCGTGTCGGGCGGCAT
CGCGGAGCGCGCGAAGTTCAACCCGCAACTCGTCGCGACGCTGATCATCGTCGGCTTCATCTATCCGTTCTTCGAAGGGA
TCGCCTGGAACGAGCGCTTCGGCGTGCAGGCCTGGCTCGCGCACGCGTTCGGCGCGCCGTTCCACGACTTCGCGGGCTCG
GTGGTCGTGCACGCGTTCGGCGGCTGGGTCGCGCTGCCGGCCGTGCTGCTGCTCGGCGCGCGCCACGGCCGCTATGCGAA
GGACGGCCGGATTGCCGCGCACCCGCCGTCGAACATCCCGTTCCTCGCGCTCGGCGCGTGGGTGCTCGCGGTCGGCTGGT
TCGGCTTCAACGTGATGAGCGCGCAGACGCTCGACAAGATCAGCGGCCTCGTCGCGGTGAACTCGCTGATGGCGATGGTC
GGCGGCACGCTCGCCGCGTGGATGGCCGGCCGCAACGACCCGGGCTTCACGTACAACGGGCCGCTCGCGGGCCTCGTCGC
GGTATGCGCGGGCTCCGACGTGATGCACCCGATCGGCGCGCTCGTCACGGGCGCCGCGGCCGGTGCGCTGTTCGTCGCGA
TGTTCACCTGCGTGCAGAACCGCTGGCGCATCGACGACGTGCTCGGCGTGTGGCCGCTGCACGGGATGTGCGGCGCACTC
GGCGGCCTCGCGGCCGGCGTGTTCGGGCTGCCGGCGCTCGGCGGCCTCGGCGGCGTGTCGTTCCTGTCGCAGCTCGTCGG
CACGCTCGGCGGCATCGCGATCGCGACCGCGGGCGGCACGCTCGTCTACGGCGCGCTGAAGGCGACCGTCGGCCTGCGTC
TCGATCGCGAAGCCGAATTCGACGGCGCCGACCTGTCGATCCACCGCATCTCGGCCACGCCCGAGCGCGATTGA

Upstream 100 bases:

>100_bases
CGCATGGCGCGGCATCCAGGCACCAATTCCACGCAATCGTTCACCAGGTTGGCTCGATATTTGCATTCCTTGCCCGTTTT
TTGCGCAAGGAAGCCACGGC

Downstream 100 bases:

>100_bases
GCGCAAACGCTTGCGATCGGCGTCCGCCAGCTCACCTGACGGACGCCCGACGCCTCATTACGTTTTCGATTCAAAAAACT
TTCATCTAGCGTCCTTACAC

Product: Rh family protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MDGLKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HNGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNERFGVQAWLAHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWMAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNRWRIDDVLGVWPLHGMCGAL
GGLAAGVFGLPALGGLGGVSFLSQLVGTLGGIAIATAGGTLVYGALKATVGLRLDREAEFDGADLSIHRISATPERD

Sequences:

>Translated_397_residues
MDGLKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HNGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNERFGVQAWLAHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWMAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNRWRIDDVLGVWPLHGMCGAL
GGLAAGVFGLPALGGLGGVSFLSQLVGTLGGIAIATAGGTLVYGALKATVGLRLDREAEFDGADLSIHRISATPERD
>Mature_397_residues
MDGLKSGVDTLFLLIGAVMVLAMHAGFAFLELGTVRKKNQVNALVKILVDFSVSTLAYFFIGYTIAYGVEFFDDIGTLSQ
HNGYALVRFFFLLTFAAAIPAIVSGGIAERAKFNPQLVATLIIVGFIYPFFEGIAWNERFGVQAWLAHAFGAPFHDFAGS
VVVHAFGGWVALPAVLLLGARHGRYAKDGRIAAHPPSNIPFLALGAWVLAVGWFGFNVMSAQTLDKISGLVAVNSLMAMV
GGTLAAWMAGRNDPGFTYNGPLAGLVAVCAGSDVMHPIGALVTGAAAGALFVAMFTCVQNRWRIDDVLGVWPLHGMCGAL
GGLAAGVFGLPALGGLGGVSFLSQLVGTLGGIAIATAGGTLVYGALKATVGLRLDREAEFDGADLSIHRISATPERD

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=405, Percent_Identity=30.8641975308642, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17550180, Length=437, Percent_Identity=29.7482837528604, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17550182, Length=441, Percent_Identity=28.7981859410431, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=431, Percent_Identity=27.6102088167053, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=439, Percent_Identity=28.0182232346241, Blast_Score=101, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6325396, Length=414, Percent_Identity=23.9130434782609, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321559, Length=415, Percent_Identity=23.6144578313253, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324187, Length=407, Percent_Identity=26.7813267813268, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI161078314, Length=417, Percent_Identity=29.2565947242206, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41510; Mature: 41510

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure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HHHHHHHHHHCCEECCCCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]