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The map label for this gene is ybfI [H]

Identifier: 116689120

GI number: 116689120

Start: 1208842

End: 1209762

Strand: Reverse

Name: ybfI [H]

Synonym: Bcen2424_1097

Alternate gene names: 116689120

Gene position: 1209762-1208842 (Counterclockwise)

Preceding gene: 116689121

Following gene: 116689119

Centisome position: 34.72

GC content: 71.23

Gene sequence:

>921_bases
ATGTCCTCCACCCGCCTCCCCGATTCCGCACGCTACTGGCGTACGCCGCTCGTGCCGGACGCGGATCTGCTCACGGCCAC
CTATCGCGATCACGCGTTCGCGCCCCACTGGCACGACGCGTACACGATCCCCGTGATTCTCGAAGGCGCGGAACGCTTCA
CCTATCGCGGCACCGGCTACGTCGCCGAAACGGGCACCGTGCCCGTGATCAATCCCGGTGAAGTGCATACGGGCTCGCGT
GCCGCCGACGAAGGCTGGTGCTATCGCGTCAGCTACATGCCGGTCGAATTCATCCACGAACTCGCGAGGTCGATCGCGGG
CCGCCCGCAGGAGGCGCCGTGGTTTGCCCCCGACGTGATCCGCGACGCCGATCTCGCGGCCCGGCTCACGCTCGCGCACC
GGATGCTGGAAGCCGGCAGCGAGCGCGCGCGGTCGTCGCAGGCCGATGCGCCCGGACAGCCGGCCGGCAATCCGGCGGCC
GGCGATTCGGCCACCGGTGCGCCGCGCATCTACGATCCGCTCGCCGCCGAAACCGCGATGCTCGACGCGCTGTCGACGCT
GATCGTGCGTCACGCCGACGTGCGGCCGCAACCGGAGCCGCTCGCGGCCGACGAGCCTCGCGTCGACGTGATGCGCGAGC
GGCTCGCCGCCGACCTGACGTCCACGGTGACGCTCGACGACGTCGCGCAGACGGTCGGCCTGTCGCCATTTCACGCGGCG
CGCCTGTTCACACGCACGACCGGCATGCCGCCGCATGCGTGGCGCAACCAGCTGCGGCTGCAGCGTGCGCTGGCGCCGCT
GCGTGCGGGCGTGCCCGTCGCCGATGTCGCCGCGGCCAGCGGCTTCGTCGACCAGAGCCACTTCACGCGGCATTTCAAAC
GGATGTTCGGCGTGCCGCCCGGGCGCTGGCAGGCAAGCTGA

Upstream 100 bases:

>100_bases
CGGTGCCGATGCCGGCCGGCACGACGATTCCGGTCAAGTAAGGCCGCACCCGCCGGGCGCGCGAGCGATACACTGTTGGT
TCGACCGCTCGCGCCCGATC

Downstream 100 bases:

>100_bases
CGCGCGGCATCCCGCCATCGCCGGCCGCGCCGCGCCCCGTGCCGGCCGGCACGTGAGCACCGGCCCCGCTCCGGCGGGCG
CAAGAACATACAAGCCGGCT

Product: AraC family transcriptional regulator

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSTRLPDSARYWRTPLVPDADLLTATYRDHAFAPHWHDAYTIPVILEGAERFTYRGTGYVAETGTVPVINPGEVHTGSR
AADEGWCYRVSYMPVEFIHELARSIAGRPQEAPWFAPDVIRDADLAARLTLAHRMLEAGSERARSSQADAPGQPAGNPAA
GDSATGAPRIYDPLAAETAMLDALSTLIVRHADVRPQPEPLAADEPRVDVMRERLAADLTSTVTLDDVAQTVGLSPFHAA
RLFTRTTGMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS

Sequences:

>Translated_306_residues
MSSTRLPDSARYWRTPLVPDADLLTATYRDHAFAPHWHDAYTIPVILEGAERFTYRGTGYVAETGTVPVINPGEVHTGSR
AADEGWCYRVSYMPVEFIHELARSIAGRPQEAPWFAPDVIRDADLAARLTLAHRMLEAGSERARSSQADAPGQPAGNPAA
GDSATGAPRIYDPLAAETAMLDALSTLIVRHADVRPQPEPLAADEPRVDVMRERLAADLTSTVTLDDVAQTVGLSPFHAA
RLFTRTTGMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS
>Mature_305_residues
SSTRLPDSARYWRTPLVPDADLLTATYRDHAFAPHWHDAYTIPVILEGAERFTYRGTGYVAETGTVPVINPGEVHTGSRA
ADEGWCYRVSYMPVEFIHELARSIAGRPQEAPWFAPDVIRDADLAARLTLAHRMLEAGSERARSSQADAPGQPAGNPAAG
DSATGAPRIYDPLAAETAMLDALSTLIVRHADVRPQPEPLAADEPRVDVMRERLAADLTSTVTLDDVAQTVGLSPFHAAR
LFTRTTGMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPPGRWQAS

Specific function: Repair Of Alkylated Guanine In DNA By Stoichiometrically Transferring The Alkyl Group At The O-6 Position To A Cysteine Residue In The Enzyme. This Is A Suicide Reaction: The Enzyme Is Irreversibly Inactivated. Can Also Repair O-4-Methylthymine. The Meth

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR003313
- InterPro:   IPR018060 [H]

Pfam domain/function: PF02311 AraC_binding; PF00165 HTH_AraC [H]

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 33415; Mature: 33284

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTRLPDSARYWRTPLVPDADLLTATYRDHAFAPHWHDAYTIPVILEGAERFTYRGTGY
CCCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCHHEEECCCCE
VAETGTVPVINPGEVHTGSRAADEGWCYRVSYMPVEFIHELARSIAGRPQEAPWFAPDVI
EECCCCEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHH
RDADLAARLTLAHRMLEAGSERARSSQADAPGQPAGNPAAGDSATGAPRIYDPLAAETAM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDALSTLIVRHADVRPQPEPLAADEPRVDVMRERLAADLTSTVTLDDVAQTVGLSPFHAA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RLFTRTTGMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPP
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCC
GRWQAS
CCCCCC
>Mature Secondary Structure 
SSTRLPDSARYWRTPLVPDADLLTATYRDHAFAPHWHDAYTIPVILEGAERFTYRGTGY
CCCCCCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEEECCHHEEECCCCE
VAETGTVPVINPGEVHTGSRAADEGWCYRVSYMPVEFIHELARSIAGRPQEAPWFAPDVI
EECCCCEEEECCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCHHHH
RDADLAARLTLAHRMLEAGSERARSSQADAPGQPAGNPAAGDSATGAPRIYDPLAAETAM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LDALSTLIVRHADVRPQPEPLAADEPRVDVMRERLAADLTSTVTLDDVAQTVGLSPFHAA
HHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
RLFTRTTGMPPHAWRNQLRLQRALAPLRAGVPVADVAAASGFVDQSHFTRHFKRMFGVPP
HHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCC
GRWQAS
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]