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The map label for this gene is citH [H]

Identifier: 116688740

GI number: 116688740

Start: 796384

End: 797661

Strand: Direct

Name: citH [H]

Synonym: Bcen2424_0717

Alternate gene names: 116688740

Gene position: 796384-797661 (Clockwise)

Preceding gene: 116688739

Following gene: 116688747

Centisome position: 22.86

GC content: 69.09

Gene sequence:

>1278_bases
ATGCATGCGGTCACGCAGTCCAGCTCCATTGCCTCGTCGCCGCGCGTGTGGCGCGCGGTGGTCGCCGCGTCGATCGGCAA
CGCGCTCGAGTGGTTCGATCTCGTCGTCTACGGCTTTTTCGCGGTGACGATCTCGAAGCTGTTCTTCCCGGCCGGCAACG
ACACCGTGTCGCTGCTGCTCACGCTCGGCACGTTCGGCGTGTCGTTCTTCATGCGCCCGCTCGGCGCGATCGTGCTCGGC
GCGTATGCCGACCGCGCGGGTCGCAAGGCCGCGCTCACGCTGTCGATCCTGCTGATGATGGCCGGCACGCTGATCATCGC
GGTGCTGCCGACCTACGGGACGATCGGCGTCGCCGCGCCGGTGATCCTCGTCGCCGCGCGGCTGATGCAGGGCTTCTCCG
CCGGCGGCGAGTTCGGCAGCGCGACCGCGTTCCTCGCCGAGCACGTGCCGGGCCGGCGCGGCTTCTTCGCGAGCTGGCAG
GTCGCGAGCCAGGGGCTCACGACGCTGCTCGCCGCGGGCTTCGGCACCGTGCTGAACGCGCAGCTCACGGCCGACCAGAT
GGCGTCGTGGGGCTGGCGCGTGCCGTTCTTCTTCGGGTTGCTGCTCGGGCCCGTCGCGTACTACATCCGCACCAAGGTCG
ACGAGACGCCCGAATTCCTCGCGGCCGAAGGCACCGCGAACCCGCTGCGCGATACGTTCGCGTCGCACAAGGCGCGCCTC
GTCGCGGCCATGGGCGTGGTCGTGCTCGGCACCGTCGCCACCTATCTCGTGCTGTTCATGCCGACCTATGGCGTGAAGCA
GCTCGGCCTCGCGCCGTCGGCCGCGTTTGCCGCGATTCTCGTGGTCGGCGTGATCCAGATGGCGTTCGCGCCGCTCGTCG
GCCACTGGTCGGATCGCTACGGCCGCGTGCGCGTGATGATCGCGCCGGCCGTCGGCATCCTCGTGCTGATCTATCCGGCG
TTCGCGTATCTCGTCGCGCATCCGGGCTTCGGCACGCTGATCGCGCTGCAGGTGCTGCTCGCGTTCCTGATGACCGGCTA
CTTCGCGGCGCTGCCGGGGCTGTTGTCCGAGGTGTTTCCGGTGCAGACGCGCACGACCGGGATGTCGCTCGCCTATAACG
TCGCGGTGACGATCTTCGGCGGCTTCGGGCCGTTCATCATCGCGTGGCTGATCCGCGAGACCGGGATGAAGACCGCGCCG
AGCTTCTATCTGATGTTCGCGGCCGTGCTGAGCCTCGCGGCGCTGGTCGTGCTGCGGCGGCGGTTCGGGTTTCGGTGA

Upstream 100 bases:

>100_bases
TAATCGCCCGAGCGCCGCATGTGGCCATGCGGCGCCGGGCAGGCCGACGGCGCGACGGCGGCCGCCTCGAACAGAACGTA
CAACGACAAGGAGATCGCCG

Downstream 100 bases:

>100_bases
AAGAGGGCGGCCGGCGCGTCAGTTGCCGCCGCGCTCGCAGACCGGTTGCGCCGGCATCAGTTGCGCGGGCGCGGCGATGG
TCTGCACCGGGCGGCCGGCG

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MHAVTQSSSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKAALTLSILLMMAGTLIIAVLPTYGTIGVAAPVILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTADQMASWGWRVPFFFGLLLGPVAYYIRTKVDETPEFLAAEGTANPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMIAPAVGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRETGMKTAP
SFYLMFAAVLSLAALVVLRRRFGFR

Sequences:

>Translated_425_residues
MHAVTQSSSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKAALTLSILLMMAGTLIIAVLPTYGTIGVAAPVILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTADQMASWGWRVPFFFGLLLGPVAYYIRTKVDETPEFLAAEGTANPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMIAPAVGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRETGMKTAP
SFYLMFAAVLSLAALVVLRRRFGFR
>Mature_425_residues
MHAVTQSSSIASSPRVWRAVVAASIGNALEWFDLVVYGFFAVTISKLFFPAGNDTVSLLLTLGTFGVSFFMRPLGAIVLG
AYADRAGRKAALTLSILLMMAGTLIIAVLPTYGTIGVAAPVILVAARLMQGFSAGGEFGSATAFLAEHVPGRRGFFASWQ
VASQGLTTLLAAGFGTVLNAQLTADQMASWGWRVPFFFGLLLGPVAYYIRTKVDETPEFLAAEGTANPLRDTFASHKARL
VAAMGVVVLGTVATYLVLFMPTYGVKQLGLAPSAAFAAILVVGVIQMAFAPLVGHWSDRYGRVRVMIAPAVGILVLIYPA
FAYLVAHPGFGTLIALQVLLAFLMTGYFAALPGLLSEVFPVQTRTTGMSLAYNVAVTIFGGFGPFIIAWLIRETGMKTAP
SFYLMFAAVLSLAALVVLRRRFGFR

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=430, Percent_Identity=32.093023255814, Blast_Score=234, Evalue=6e-63,
Organism=Escherichia coli, GI1788942, Length=434, Percent_Identity=35.7142857142857, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1788292, Length=391, Percent_Identity=31.4578005115089, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789941, Length=427, Percent_Identity=33.9578454332553, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87082231, Length=459, Percent_Identity=25.2723311546841, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI87082404, Length=382, Percent_Identity=24.3455497382199, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 45180; Mature: 45180

Theoretical pI: Translated: 10.45; Mature: 10.45

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCC
>Mature Secondary Structure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HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]