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The map label for this gene is yiaJ [H]

Identifier: 116688640

GI number: 116688640

Start: 689001

End: 689900

Strand: Direct

Name: yiaJ [H]

Synonym: Bcen2424_0617

Alternate gene names: 116688640

Gene position: 689001-689900 (Clockwise)

Preceding gene: 116688639

Following gene: 116688641

Centisome position: 19.78

GC content: 70.11

Gene sequence:

>900_bases
ATGACCAAGATGCCAGACTCTCTTGCCCTCGACGCCCGGCGCGCGATGCCGGCCGATACGTCGCTGACCGACAGCATCGG
CGCGACCAGCACGCCGCTCGACCTCGCCGCGCAGCAGGCCGGCACGCAGACATTGCTGCGCGGCCTCGCGATCCTCGAGG
CGATCGCCAATGGCGCGCGCGACATGCGCGCGATCGGCGCCGCGCTCGGCACGACGCGCAGCACGACGCACCGGCTCGTC
AGCAGCCTCGTGCAGGCGCGCTATCTGCGCCAGGTGCAGGGCGGCTACCTGCTCGGCCCGAAGCTGATCGAGCTCGGCAC
GATCGCGCTCGAGCAGATGCCGCTCACCGCGGTGGCGCGTCCGCATCTCGAGGCGCTCGCGGAAGCGACGCTCGACACGA
TCCATCTCGGCGTGCGCGACGGCGACGACGTGCTGTACATCGACAAGATTCCCGGTACGCGCGGCCTCGAGATGCGCTCG
CGCGTCGGCCACCGGATGCCGCTCGCGTCGACCGGCATCGGCAAGGCGATGATGCTCGACCTCGATCCGGACCTGTGGCG
CTCGCTGTTCGAAGCCGCGCGGCGCGCGCTGGCCGGCGTCAACTTCAAGCCCGACAACCGGCCCGAGACGAGCGCGTTCC
TGCAGCGCATGGCCCACTACGCGGCCGGCGGCTATACGTTCGATCTCGAGGAAAACGAGGCGTCGATCCGCTGCGTGGCC
GCGCCGATCCGCGACGCGTCGGGCGCGATCGTCGCGGCGGTGTCGGTCGCGAGCACGATTCCGTACATGCCGCACGACCG
CATGGACGAACTGATTCCGCTCGTGCAGCGCGAAGCGCGAGCCATTTCCGCGGAACTGGGCTGGAGCCCGCCGCAGGGTA
CCCGCAGGATCAAACGATGA

Upstream 100 bases:

>100_bases
GCTTTACTCGGTGCATCGACCGCCGCCACGCCGCCGCATCGGACGCAGTTCCGGCGCCGCGCGAGCGGCTTCACCGATTC
CGTTGAAGAAGCCCCGTCCC

Downstream 100 bases:

>100_bases
CGACTTCGACCCGGCCCGCTCCGGATGCCAGTTCCGCCTCCCCGTCGCTGATCGCGCTCGACTGGGGCACCACGTCGCTG
CGCGCGTATCTGTACGACGC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: YiaKLMNOPQRS operon repressor; YiaK-S operon repressor [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MTKMPDSLALDARRAMPADTSLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLV
SSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRS
RVGHRMPLASTGIGKAMMLDLDPDLWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR

Sequences:

>Translated_299_residues
MTKMPDSLALDARRAMPADTSLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLV
SSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRS
RVGHRMPLASTGIGKAMMLDLDPDLWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
>Mature_298_residues
TKMPDSLALDARRAMPADTSLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGARDMRAIGAALGTTRSTTHRLVS
SLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVARPHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSR
VGHRMPLASTGIGKAMMLDLDPDLWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVAA
PIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR

Specific function: Negatively controls the transcription of the yiaKLMNOPQRS operon, which may be involved in the utilization of 2,3-diketo-L-gulonate [H]

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1789999, Length=258, Percent_Identity=32.1705426356589, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1788131, Length=266, Percent_Identity=31.9548872180451, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=29.6, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082359, Length=252, Percent_Identity=29.3650793650794, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788581, Length=249, Percent_Identity=30.9236947791165, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786715, Length=254, Percent_Identity=27.5590551181102, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790752, Length=256, Percent_Identity=23.4375, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 32243; Mature: 32111

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKMPDSLALDARRAMPADTSLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGAR
CCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DMRAIGAALGTTRSTTHRLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHH
PHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLD
HHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
LDPDLWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
ECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure 
TKMPDSLALDARRAMPADTSLTDSIGATSTPLDLAAQQAGTQTLLRGLAILEAIANGAR
CCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
DMRAIGAALGTTRSTTHRLVSSLVQARYLRQVQGGYLLGPKLIELGTIALEQMPLTAVAR
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCHHHHHH
PHLEALAEATLDTIHLGVRDGDDVLYIDKIPGTRGLEMRSRVGHRMPLASTGIGKAMMLD
HHHHHHHHHHHHHHEEECCCCCCEEEEECCCCCCCCHHHHHCCCCCCCCCCCCCCEEEEE
LDPDLWRSLFEAARRALAGVNFKPDNRPETSAFLQRMAHYAAGGYTFDLEENEASIRCVA
CCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCEEEEE
APIRDASGAIVAAVSVASTIPYMPHDRMDELIPLVQREARAISAELGWSPPQGTRRIKR
ECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]