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The map label for this gene is hisH [H]

Identifier: 116688453

GI number: 116688453

Start: 478751

End: 479392

Strand: Direct

Name: hisH [H]

Synonym: Bcen2424_0429

Alternate gene names: 116688453

Gene position: 478751-479392 (Clockwise)

Preceding gene: 116688452

Following gene: 116688454

Centisome position: 13.74

GC content: 66.67

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCGATTGTGGATTATGGGATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCAAGAAGGCCGAACC
GGCCGCCGACGTGGCGATCGTCGACACGCCGGCCGCGATTCGCGCGGCCGACCGCGTCGTGCTGCCCGGCCAGGGCGCGA
TGCCCGACTGCATGCGCTGTCTCGGCGAATCGGGCCTGCAGGAGGCCGTGATCGAGGCGTCGCGCACGAAGCCGCTGCTC
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGTGCGGAAGGCGACACGAAGGGCCTCGGCCTGTTGCCGGGCAA
GGTCGTGCGCTTCGAACTCGACGGCCGCGTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGCG
TGCGCCAGGCGCAGCCGCACCCGCTGTGGGACGGCGTGCCCGATGACGCGTATTTCTACTTCGTGCACAGCTATTACGTG
AGCCCGGACAACCCGGCGCATACGGTCGGCGAAACGGCCTACGGCGCGCCGTTTACGTCCGCGGTCGCGCGGGACAATCT
CTTCGCGACCCAATTCCACCCCGAGAAAAGCGCGGAGGTCGGGTTGCGTCTGTATCGCAACTTCGTACACTGGAAACCGT
AA

Upstream 100 bases:

>100_bases
CCGGCATCAACATCGCCACACGGCTGATGGGTCTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCAATGGACTGAGG
GCGCTACTGCCTGCACTGAG

Downstream 100 bases:

>100_bases
ACGTGGTGCGGGAGCCACAGTCGACGGCGCGCAAGCGCCTGTCGTGCGGGGCTCTCACGCTTAAGCGCCGAAAGAGTTGT
ACTAAACTAGCGAGACGGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRVQDDGSRFKVPQMGWNRVRQAQPHPLWDGVPDDAYFYFVHSYYV
SPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRVQDDGSRFKVPQMGWNRVRQAQPHPLWDGVPDDAYFYFVHSYYV
SPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVIEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRVQDDGSRFKVPQMGWNRVRQAQPHPLWDGVPDDAYFYFVHSYYV
SPDNPAHTVGETAYGAPFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=212, Percent_Identity=35.8490566037736, Blast_Score=120, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=31.651376146789, Blast_Score=101, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23322; Mature: 23322

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVIEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRVQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEEECC
GSRFKVPQMGWNRVRQAQPHPLWDGVPDDAYFYFVHSYYVSPDNPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVIEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRVQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEEECC
GSRFKVPQMGWNRVRQAQPHPLWDGVPDDAYFYFVHSYYVSPDNPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHCCCCCCCCCCCCCCEEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]