Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 116688302

GI number: 116688302

Start: 320762

End: 321937

Strand: Direct

Name: livK [C]

Synonym: Bcen2424_0278

Alternate gene names: 116688302

Gene position: 320762-321937 (Clockwise)

Preceding gene: 116688301

Following gene: 116688304

Centisome position: 9.21

GC content: 63.35

Gene sequence:

>1176_bases
ATGGCGTTTTTTGGGACCGAACTGGAGACTTACATGAATATCAAGATGCAAAAGCTGTTGCCGATCACCGCGGCAGCGAT
GCTGTGCGCTGCAGCTGCAAGCAACGCGTCCGCCGATCAAGTCGTCAAGATCGGCCACGTCGCGCCGTTGACGGGCGGCA
TTGCACACCTGGGCAAGGACAACGAAAACGGTGCACGTCTCGCAGTCGAGGAGATCAACGCGAAGGGCCTCACGATCGGC
GGCCAGAAGATCACGCTGCAACTCGACCCGCAGGATGACGCCGCCGACCCGCGGCAGGCGACGCAGGTTGCGCAGAAGCT
CGTCGACGACAAGGTCGTCGCGGTGGTCGGCCACCTGAACTCGGGCACGTCGATCCCGGCCTCGAAGATCTACAGCGACG
CGGGCATCGTGCAGATCTCGCCGTCGGCGACCAACCCGGCGTACACGCAGCAAGGCTTCAAGACCACGTACCGCGTGGTG
GCGACCGATGCGCAGCAGGGCCCGGCACTGGCGAACTACGCGCACTCGAAGGGCATCAAGAGCGTGGCGGTGGTCGACGA
TTCGACCGCGTACGGCCAGGGTCTCGCGAACGAGTTCGAGAAGAAGGCGAAGGCGCTCGGCCTGAAGGTGATGTCGCATG
ACGCGACGAACGACAAGGCGGTCGACTTCCGCGCGATTCTGACCAAGATCAAGGGCGAAAACCCGGACGCGATCATGTAC
GGCGGCATGGACGCCACCGGCGGTCCGTTCGCGAAACAGGCGAAGCAGCTCGGCCTGCGCGCGAAGATCTTCGCGGGCGA
CGGCGTGTGCACGGAAAAGCTGGCCGACCTGGCCGGCGACGCGACCGACAACATCGTGTGCTCGGAAGCCGGTGCTTCGC
TCGAGAAGATGCCGGGCGGCAACGCGTTCAAGGCGAAGTACGAGAAGCGCTTCGGCCAGCCGATCCAGATCTATGCACCG
TTCACGTATGACGCCGTGTACATCATCGCCGACGCGATGAAGCGCGCGAACTCGACGGACCCGGACAAGATCCTCGCGGC
GATGCCGGCGACGAAGTACACGGGCGTGATCGGCACGACGACCTTCGACTCGAAGGGCGACCTGCAGCACGGCGTGATCT
CGCTGTACAACTACAAGAGCGGCAAGAAGTCGCTGCTCGACGAAGTGAAGATGTAA

Upstream 100 bases:

>100_bases
AAGCGGCTGTTTTCACGTGCTTGAGGCCAAGTGCATGAACGATGCAACCGGCGAATCGTCCAGTGATTGAAACACGGGGA
TGGCAGCGGGGCACCGGGCG

Downstream 100 bases:

>100_bases
CGCGACGCGCGATCGACAAGCGGTCGACAGGAGGGTGAAAGCGCGCATGCGGCAACGCATGCGCGCTTTCTTTTTGGGCC
GGCGCGCGCGGCCGCGCCGT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MAFFGTELETYMNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIG
GQKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVV
ATDAQQGPALANYAHSKGIKSVAVVDDSTAYGQGLANEFEKKAKALGLKVMSHDATNDKAVDFRAILTKIKGENPDAIMY
GGMDATGGPFAKQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNIVCSEAGASLEKMPGGNAFKAKYEKRFGQPIQIYAP
FTYDAVYIIADAMKRANSTDPDKILAAMPATKYTGVIGTTTFDSKGDLQHGVISLYNYKSGKKSLLDEVKM

Sequences:

>Translated_391_residues
MAFFGTELETYMNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIG
GQKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVV
ATDAQQGPALANYAHSKGIKSVAVVDDSTAYGQGLANEFEKKAKALGLKVMSHDATNDKAVDFRAILTKIKGENPDAIMY
GGMDATGGPFAKQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNIVCSEAGASLEKMPGGNAFKAKYEKRFGQPIQIYAP
FTYDAVYIIADAMKRANSTDPDKILAAMPATKYTGVIGTTTFDSKGDLQHGVISLYNYKSGKKSLLDEVKM
>Mature_390_residues
AFFGTELETYMNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGG
QKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVA
TDAQQGPALANYAHSKGIKSVAVVDDSTAYGQGLANEFEKKAKALGLKVMSHDATNDKAVDFRAILTKIKGENPDAIMYG
GMDATGGPFAKQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNIVCSEAGASLEKMPGGNAFKAKYEKRFGQPIQIYAPF
TYDAVYIIADAMKRANSTDPDKILAAMPATKYTGVIGTTTFDSKGDLQHGVISLYNYKSGKKSLLDEVKM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=366, Percent_Identity=32.7868852459016, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI48994941, Length=374, Percent_Identity=32.620320855615, Blast_Score=175, Evalue=6e-45,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41190; Mature: 41059

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFFGTELETYMNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKD
CCEECCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCHHHCCCC
NENGARLAVEEINAKGLTIGGQKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLN
CCCCCEEEEEECCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECC
SGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYAHSKGIK
CCCCCCHHHHHCCCCEEEECCCCCCCHHHHHCHHEEEEEEEECCCCCCHHHHHHHHCCCC
SVAVVDDSTAYGQGLANEFEKKAKALGLKVMSHDATNDKAVDFRAILTKIKGENPDAIMY
EEEEEECCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEE
GGMDATGGPFAKQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNIVCSEAGASLEKMPGG
CCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCC
NAFKAKYEKRFGQPIQIYAPFTYDAVYIIADAMKRANSTDPDKILAAMPATKYTGVIGTT
CHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEC
TFDSKGDLQHGVISLYNYKSGKKSLLDEVKM
CCCCCCCCCCCEEEEEECCCCHHHHHHHHCC
>Mature Secondary Structure 
AFFGTELETYMNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKD
CEECCCHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCHHHCCCC
NENGARLAVEEINAKGLTIGGQKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLN
CCCCCEEEEEECCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECC
SGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYAHSKGIK
CCCCCCHHHHHCCCCEEEECCCCCCCHHHHHCHHEEEEEEEECCCCCCHHHHHHHHCCCC
SVAVVDDSTAYGQGLANEFEKKAKALGLKVMSHDATNDKAVDFRAILTKIKGENPDAIMY
EEEEEECCCHHHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEE
GGMDATGGPFAKQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNIVCSEAGASLEKMPGG
CCCCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCC
NAFKAKYEKRFGQPIQIYAPFTYDAVYIIADAMKRANSTDPDKILAAMPATKYTGVIGTT
CHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCEEEEC
TFDSKGDLQHGVISLYNYKSGKKSLLDEVKM
CCCCCCCCCCCEEEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA