Click here to switch to the map view.

The map label for this gene is dhbE [H]

Identifier: 116688199

GI number: 116688199

Start: 193504

End: 195177

Strand: Direct

Name: dhbE [H]

Synonym: Bcen2424_0175

Alternate gene names: 116688199

Gene position: 193504-195177 (Clockwise)

Preceding gene: 116688198

Following gene: 116688200

Centisome position: 5.55

GC content: 60.51

Gene sequence:

>1674_bases
ATGACCGCCTCATCCCATGCCTCTCGCGACGTGTTCGACGGCGTGACGCCGTTTCCCGCCGACTACGCCGCCCGTTACCG
TGCGAAAGGTTATTGGCAGGACAAGCCGATGCGCCAGGTTTTCGGCGAATGGTGCCAGGCGCACGCGCCCAAAATCGCCT
TGCGCGACGCGACGCGCAGTGTGACTTTCGCGGAACTCGACGCGGCATCGACCAATCTCGCGCTTAACCTGCTTGATGTA
GGCATTCTTCCGGGCGACAAGCTCGTCGTGCAGTTGCCGAACGTCATCGAATTCGTGTATTTGCACTTTGCGTTGCAGAA
GATCGGCGCGATACCGGTGCTTGCGCTGCCGCCGCATCGCTACCGCGAGATCAGCTGCTTCGTCGAGATGGCGGAAGCGG
TCGCGCTCATCACGCCCGACGCACAAGGCGATTTCAGATACACGCAGATGACGGAACGCCTGCGCAACGAGGCACCGCGT
CTTCGCTTCGGCATCGTGTTAGGAGACGCACCCGAGGGCTTTGTCTCGCTACGCGACCTGATCGAGCGTCCTCCGGCACG
CGATGCGAGCGAACTGGACAACATCGCAATCGACCCATGCTCGCCGGCTCTCTTCTTGCTGTCCGGCGGCACCACCGGCA
TTCCCAAGCTGATCCCGCGCACCCATAACGACTACGTGCTCAACTCGAAGCTGGCCGCGGCCGTCTGCGATATACGCGAC
GGTGACGTCTTGCTCGATGTGCTCCCGATCGGTCACAACCTGCCGCTAGCGTGTCCCGGTCTGCAAGGTTTTCTGTCGCG
CGGCTGCACTGTCGTGCTGCATACGAGTACGTCGTCCGACCAGATCTTTCCGCTTATCGACCGCTACAAGGTCACGCATA
TCCATGTTGTGCCTGCACTGCTGATCCGCTGGCTGGAATCGCCGGTGATTGCGGACTTCGATCTCGGCTCGGTTCGGGTG
ATTCAGAGCGGCGGCCAGCGCTTGCAGCCTGAAACGCGCAAACGCACCCAGCAGCGGTTGCCGCAATGCTTCGTGCAGGA
GAACTTCGGCATGGCCGAGGGCGTGCTGATGTTCGTGCGTGCCGACGATCCGCTCGAAGTGCGCATGGAAACCGTCGGGC
GACCCGTTTGCGAAGACGACGAAGTCATGCTACTCGGTGAGGACGATCAGCCGGTACAGCCTGGCGAAGTGGGCGAACTG
TGCTGCCGCGGTCCGTACACTTTGCGCGGTTACTACCGTGCGCCCGAGCACAACGCACGCGCGTTCACGTCCGACGGCTT
TTATCGCAGCGGCGACCTCATGAGGCAACATCCGTCGGGCAACTACATTGTTGAAGGACGCAAGAAGGACCTGATCAATC
GCGGCGCGGAAAAGATCAGTGCAGAAGAGATCGAGAACCTGATTCTTGCGCACCCGGCCGTGGTGAACGTTGCCTGCGTG
GCGATGCCCGATCCGCTGATGGGAGAGCGCAATTGCGCGTGTCTGATTCTGCGTGAGGGCGTTTCATTGTCGCTCGACGA
ACTCGTTGCATTCCTGAAAAATCAGGAGATCGCCAAGTTCAAGCTTCCCGAACGCGTTGAGATCTTCACGTCATTTCCGC
TTTCGAATTTCGGTAAGGTCTCGAAGAAAGATCTGGCCGCAACGGTGGCCGAGCGCGTACAGGCGGCGCAATGA

Upstream 100 bases:

>100_bases
GACGATGCGCTTCGTCGACGGTCAGGTGATCGTCATCGACGGCGGCTACAGCTCGATCACCTGATCGACGCGCCCCCTGC
CCCCACTCAAGGAGACACTG

Downstream 100 bases:

>100_bases
AATGCTATGTAACCGCAATCCACGAGAACGGGATCGCCACGCTCACGCTTGCCGATCCCGAGCGTCGTAATGTCCTTTCC
GAGGTGCTTTGCGAACAGTT

Product: AMP-dependent synthetase and ligase

Products: diphosphate; (2,3-dihydroxybenzoyl)adenylate

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MTASSHASRDVFDGVTPFPADYAARYRAKGYWQDKPMRQVFGEWCQAHAPKIALRDATRSVTFAELDAASTNLALNLLDV
GILPGDKLVVQLPNVIEFVYLHFALQKIGAIPVLALPPHRYREISCFVEMAEAVALITPDAQGDFRYTQMTERLRNEAPR
LRFGIVLGDAPEGFVSLRDLIERPPARDASELDNIAIDPCSPALFLLSGGTTGIPKLIPRTHNDYVLNSKLAAAVCDIRD
GDVLLDVLPIGHNLPLACPGLQGFLSRGCTVVLHTSTSSDQIFPLIDRYKVTHIHVVPALLIRWLESPVIADFDLGSVRV
IQSGGQRLQPETRKRTQQRLPQCFVQENFGMAEGVLMFVRADDPLEVRMETVGRPVCEDDEVMLLGEDDQPVQPGEVGEL
CCRGPYTLRGYYRAPEHNARAFTSDGFYRSGDLMRQHPSGNYIVEGRKKDLINRGAEKISAEEIENLILAHPAVVNVACV
AMPDPLMGERNCACLILREGVSLSLDELVAFLKNQEIAKFKLPERVEIFTSFPLSNFGKVSKKDLAATVAERVQAAQ

Sequences:

>Translated_557_residues
MTASSHASRDVFDGVTPFPADYAARYRAKGYWQDKPMRQVFGEWCQAHAPKIALRDATRSVTFAELDAASTNLALNLLDV
GILPGDKLVVQLPNVIEFVYLHFALQKIGAIPVLALPPHRYREISCFVEMAEAVALITPDAQGDFRYTQMTERLRNEAPR
LRFGIVLGDAPEGFVSLRDLIERPPARDASELDNIAIDPCSPALFLLSGGTTGIPKLIPRTHNDYVLNSKLAAAVCDIRD
GDVLLDVLPIGHNLPLACPGLQGFLSRGCTVVLHTSTSSDQIFPLIDRYKVTHIHVVPALLIRWLESPVIADFDLGSVRV
IQSGGQRLQPETRKRTQQRLPQCFVQENFGMAEGVLMFVRADDPLEVRMETVGRPVCEDDEVMLLGEDDQPVQPGEVGEL
CCRGPYTLRGYYRAPEHNARAFTSDGFYRSGDLMRQHPSGNYIVEGRKKDLINRGAEKISAEEIENLILAHPAVVNVACV
AMPDPLMGERNCACLILREGVSLSLDELVAFLKNQEIAKFKLPERVEIFTSFPLSNFGKVSKKDLAATVAERVQAAQ
>Mature_556_residues
TASSHASRDVFDGVTPFPADYAARYRAKGYWQDKPMRQVFGEWCQAHAPKIALRDATRSVTFAELDAASTNLALNLLDVG
ILPGDKLVVQLPNVIEFVYLHFALQKIGAIPVLALPPHRYREISCFVEMAEAVALITPDAQGDFRYTQMTERLRNEAPRL
RFGIVLGDAPEGFVSLRDLIERPPARDASELDNIAIDPCSPALFLLSGGTTGIPKLIPRTHNDYVLNSKLAAAVCDIRDG
DVLLDVLPIGHNLPLACPGLQGFLSRGCTVVLHTSTSSDQIFPLIDRYKVTHIHVVPALLIRWLESPVIADFDLGSVRVI
QSGGQRLQPETRKRTQQRLPQCFVQENFGMAEGVLMFVRADDPLEVRMETVGRPVCEDDEVMLLGEDDQPVQPGEVGELC
CRGPYTLRGYYRAPEHNARAFTSDGFYRSGDLMRQHPSGNYIVEGRKKDLINRGAEKISAEEIENLILAHPAVVNVACVA
MPDPLMGERNCACLILREGVSLSLDELVAFLKNQEIAKFKLPERVEIFTSFPLSNFGKVSKKDLAATVAERVQAAQ

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=508, Percent_Identity=25.3937007874016, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI187761345, Length=384, Percent_Identity=29.9479166666667, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI187761343, Length=384, Percent_Identity=29.9479166666667, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI38505220, Length=504, Percent_Identity=23.2142857142857, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=23.4126984126984, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=23.4126984126984, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI58082049, Length=504, Percent_Identity=23.015873015873, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI115511026, Length=483, Percent_Identity=23.1884057971014, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI28416953, Length=533, Percent_Identity=24.577861163227, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI227499619, Length=489, Percent_Identity=23.3128834355828, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI122937307, Length=504, Percent_Identity=23.015873015873, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI42544132, Length=496, Percent_Identity=21.3709677419355, Blast_Score=94, Evalue=5e-19,
Organism=Homo sapiens, GI8923896, Length=546, Percent_Identity=23.2600732600733, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI253970489, Length=559, Percent_Identity=22.7191413237925, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786810, Length=542, Percent_Identity=41.5129151291513, Blast_Score=390, Evalue=1e-109,
Organism=Escherichia coli, GI145693145, Length=538, Percent_Identity=29.9256505576208, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI221142682, Length=522, Percent_Identity=26.6283524904215, Blast_Score=155, Evalue=5e-39,
Organism=Escherichia coli, GI1788107, Length=552, Percent_Identity=23.9130434782609, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1790505, Length=533, Percent_Identity=23.4521575984991, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1786529, Length=537, Percent_Identity=23.2774674115456, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1789201, Length=377, Percent_Identity=24.6684350132626, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788595, Length=350, Percent_Identity=24.5714285714286, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994703, Length=522, Percent_Identity=25.095785440613, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=522, Percent_Identity=25.095785440613, Blast_Score=139, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=522, Percent_Identity=25.095785440613, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17560308, Length=517, Percent_Identity=26.4990328820116, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17531443, Length=360, Percent_Identity=26.6666666666667, Blast_Score=132, Evalue=4e-31,
Organism=Caenorhabditis elegans, GI17557194, Length=548, Percent_Identity=25.3649635036496, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=540, Percent_Identity=25.1851851851852, Blast_Score=122, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=23.7911025145068, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=25.4826254826255, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=515, Percent_Identity=24.4660194174757, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=503, Percent_Identity=26.441351888668, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=358, Percent_Identity=24.5810055865922, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=356, Percent_Identity=23.314606741573, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71983001, Length=537, Percent_Identity=23.463687150838, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI71982997, Length=537, Percent_Identity=23.463687150838, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI193209603, Length=271, Percent_Identity=23.2472324723247, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17556356, Length=339, Percent_Identity=22.7138643067847, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6319699, Length=354, Percent_Identity=30.225988700565, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323903, Length=327, Percent_Identity=24.7706422018349, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24581924, Length=515, Percent_Identity=26.0194174757282, Blast_Score=149, Evalue=6e-36,
Organism=Drosophila melanogaster, GI18859661, Length=531, Percent_Identity=23.5404896421846, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI20130357, Length=410, Percent_Identity=25.609756097561, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356441, Length=379, Percent_Identity=25.8575197889182, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI21355181, Length=523, Percent_Identity=22.9445506692161, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21358303, Length=379, Percent_Identity=23.2189973614776, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281365686, Length=382, Percent_Identity=23.2984293193717, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648253, Length=321, Percent_Identity=23.98753894081, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922652, Length=518, Percent_Identity=23.5521235521236, Blast_Score=91, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648255, Length=321, Percent_Identity=24.2990654205607, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI21356947, Length=385, Percent_Identity=20.2597402597403, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648260, Length=389, Percent_Identity=23.9074550128535, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24653035, Length=384, Percent_Identity=21.6145833333333, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI161076582, Length=315, Percent_Identity=21.9047619047619, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58

Molecular weight: Translated: 61610; Mature: 61478

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSHASRDVFDGVTPFPADYAARYRAKGYWQDKPMRQVFGEWCQAHAPKIALRDATRS
CCCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
VTFAELDAASTNLALNLLDVGILPGDKLVVQLPNVIEFVYLHFALQKIGAIPVLALPPHR
EEEEECCCCCCCEEEEEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEECCCHH
YREISCFVEMAEAVALITPDAQGDFRYTQMTERLRNEAPRLRFGIVLGDAPEGFVSLRDL
HHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHH
IERPPARDASELDNIAIDPCSPALFLLSGGTTGIPKLIPRTHNDYVLNSKLAAAVCDIRD
HCCCCCCCHHHHCCEEECCCCCEEEEEECCCCCCCHHCCCCCCCEEECCHHHHHEECCCC
GDVLLDVLPIGHNLPLACPGLQGFLSRGCTVVLHTSTSSDQIFPLIDRYKVTHIHVVPAL
CCEEEEEEECCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCHHHCEEEEEEHHHHHH
LIRWLESPVIADFDLGSVRVIQSGGQRLQPETRKRTQQRLPQCFVQENFGMAEGVLMFVR
HHHHHCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
ADDPLEVRMETVGRPVCEDDEVMLLGEDDQPVQPGEVGELCCRGPYTLRGYYRAPEHNAR
CCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCC
AFTSDGFYRSGDLMRQHPSGNYIVEGRKKDLINRGAEKISAEEIENLILAHPAVVNVACV
EEECCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHEEEEE
AMPDPLMGERNCACLILREGVSLSLDELVAFLKNQEIAKFKLPERVEIFTSFPLSNFGKV
ECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCCC
SKKDLAATVAERVQAAQ
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TASSHASRDVFDGVTPFPADYAARYRAKGYWQDKPMRQVFGEWCQAHAPKIALRDATRS
CCCCCCCCHHHCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCC
VTFAELDAASTNLALNLLDVGILPGDKLVVQLPNVIEFVYLHFALQKIGAIPVLALPPHR
EEEEECCCCCCCEEEEEEEEECCCCCCEEEECHHHHHHHHHHHHHHHHCCCCEEECCCHH
YREISCFVEMAEAVALITPDAQGDFRYTQMTERLRNEAPRLRFGIVLGDAPEGFVSLRDL
HHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHH
IERPPARDASELDNIAIDPCSPALFLLSGGTTGIPKLIPRTHNDYVLNSKLAAAVCDIRD
HCCCCCCCHHHHCCEEECCCCCEEEEEECCCCCCCHHCCCCCCCEEECCHHHHHEECCCC
GDVLLDVLPIGHNLPLACPGLQGFLSRGCTVVLHTSTSSDQIFPLIDRYKVTHIHVVPAL
CCEEEEEEECCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCCCCHHHCEEEEEEHHHHHH
LIRWLESPVIADFDLGSVRVIQSGGQRLQPETRKRTQQRLPQCFVQENFGMAEGVLMFVR
HHHHHCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
ADDPLEVRMETVGRPVCEDDEVMLLGEDDQPVQPGEVGELCCRGPYTLRGYYRAPEHNAR
CCCCHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHCCCCEEEEEEECCCCCCC
AFTSDGFYRSGDLMRQHPSGNYIVEGRKKDLINRGAEKISAEEIENLILAHPAVVNVACV
EEECCCCCCCCCHHHCCCCCCEEECCCHHHHHHHHHHHCCHHHHHHHHHCCCHHHEEEEE
AMPDPLMGERNCACLILREGVSLSLDELVAFLKNQEIAKFKLPERVEIFTSFPLSNFGKV
ECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEECCCCCEEEEECCCCCCCCCC
SKKDLAATVAERVQAAQ
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 2,3-dihydroxybenzoate

Specific reaction: ATP + 2,3-dihydroxybenzoate = diphosphate + (2,3-dihydroxybenzoyl)adenylate

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]