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The map label for this gene is priA [H]

Identifier: 116688136

GI number: 116688136

Start: 115255

End: 117516

Strand: Direct

Name: priA [H]

Synonym: Bcen2424_0112

Alternate gene names: 116688136

Gene position: 115255-117516 (Clockwise)

Preceding gene: 116688135

Following gene: 116688137

Centisome position: 3.31

GC content: 72.28

Gene sequence:

>2262_bases
ATGAGCGGTACCTACCTGCGCGTGGCGCTCGACCATCCGCTCGCGACCCTGTTCGACTATCGCTGCGACGTGCAGCCGGC
GCCCGTGCCCGGGACGCTCGTGCAAGTGCCGTTCGGCAAGCGGCAGGCCGTCGGGCTCGTTTGCGAAGTCACCACTCACA
CCGATGTCCCGCCGTCGCGACTGCGCGCGGTCGATGCGATCTGTACGGAGCTGCCGCCATTGTCGGCGGACTGGCTCGCG
CTCGTGTCGTTCGCCGCCGACTACTATCAGCGCGGGCGCGGCGAGGTCGCGTTGCCGGCGCTGCCGCAGGCGCTGCGCGA
CGCCGGGCGCTGGGGGCGGCTGCTCGCCCCGGAAGTGCGCTACCGGCCGAACGAAGCTGGCCGCGCGGCGCTGCCCGATG
CGTTGCCCGCGCGCGGCGCCGCGCTGCGCCGGCTCGCGCAGGCGCTGGTCGATACCGGTTCGCTGACGCTGCCCGACGCG
CGCGCGCTGCACCCGAAGGCGGCTGCGACGCTCGACGACTGGGCGGCGCGCGGCTGGGTGGACGTCGAGGAGATCGGCTG
GGCCGATGCGCCTGTGGTCAACCCTGTGGATAACCTGTCGACAGGCAGTGGACGCGCTGTGCCGCCGGCGTTGACGGACC
AGCAGGCCGAGGCGCTCGACGCGATTCGCGCGGCGCAAGGCTTCGCGCCGTTCCTGCTGCACGGCGTGACGGGCAGTGGC
AAGACCGAGGTCTATCTGCATGCGCTCGCGTCGCTGCTCGACGCGCGGCCGGATGCGCAGGCGCTGGTGCTCGTGCCCGA
AATCAACCTGACGCCGCAGTTCGAGGCCGCGTTTCGCGCGCGCTTCGCGGGCGCGCTCGCCGACGACGCGATCGTCACGC
TGCACAGCGGGCTCGCCGAAGGCGAACGGGCGCGCAACTGGCTCGCCGCGCACACGGGCCGCGCGCGAATCGTGCTCGGC
ACGCGTCTCGCGGTGCTCGCGTCGATGCCGACGCTCGCGCTGATCGTCGTCGACGAGGAGCACGAGCCCGCGTACAAGCA
GCAGGAAGGGCTGCGCTATTCGGCGCGCGACCTGGCCGTGTGGCGCGCGAAGCAGCTCGGCATCACCGTCGTGCTCGGCT
CGGCCACGCCGTCGCTCGAAAGCTGGTGGCAGGCCGAGCAGGGGCGTTACACGCGGCTCACGCTGTCGCGTCGCGCGGTG
GCCGACGCGACGCTGCCGACCGTGCGGCTGATCGATCTCGAAGAGGAACGGCGGCGCGGGCGCGCGTCGATGGGCGGGCT
GTCGGGGCCGCTCGTCGCGGCGCTGAAGGCGCGGCTCGAACGCGGCGAACAGAGTCTCGTGTTCCTGAACCGGCGCGGTT
ACGCGCCCCAGCTCGCGTGCGACGCATGCGGCTGGGTGGCCGGCTGCCCGCGTTGCAGCGCGTACGTCGTGCTGCACAAG
CCCGAGCATGCGCTGCGCTGCCATCACTGCGGCTGGGAAGCGCGCATTCCGCGTTCGTGCCCCGAGTGCGGAAACGTCGA
CATCGCGCCGCTCGGGCGCGGCACGCAGCGCATCGAGGAGGCGCTCGCCGAGGCCGTGCCGGGCGCGCGGATTCTGCGGA
TCGATGCCGACAGCACGCGCCGCAAGGGCAGCGCACAGGCGCTTTTTTCCGACGTGCACGCGGGCGAAGTCGACATCCTG
GTCGGCACGCAGATGATCGCGAAAGGGCACGACTTCCAGCGCGTGTCGCTTGTCGGCGTGCTCAATGCCGATACCGCGCT
GTTCTCGCACGACTTCCGCGCGAGCGAGCGGCTGTTCGCGCAGCTGATGCAGGTGAGCGGCCGCGCGGGGCGCGCCGGAC
TGCCGGGCGAGGTGATGGTGCAGACGCGCTACCCGCGTCACGCGCTGTATCACGCGCTCGGGCGGCAGGACTACGTGGGC
TTCGCGAATTCGACGCTCGCCGAGCGCCGCGACGCGCACCTGCCGCCGTTCGTCTACCAGGCGCTGCTGCGCGCCGAAGG
GCGCACGCTCGACGCGGCGCTCGCGTTCCTGCTGCAGGCCGCGGCCGCGTTGCCCGGGCTGCCGGGCGCCGATCGCGTGA
CCGTGTACGACGCGGTGCCGATGACGATCGTCAAGGTCGCGAATGTCCACCGTGCGCAGCTGCTGCTCGAAAGCGCGTCG
CGGGCGGCGCTGCAGCACGCGCTGCGCGCATGGCAGCCGGAACTGCGCGCGCTGAAGGGCGTGCTGCGGTGGAGCGTCGA
GGTCGATCCGCTGGATATCTGA

Upstream 100 bases:

>100_bases
TAGCGCCGAATCTGAAGTTTTACTTTAAGTTCGCCGTCACGATGCGGGCGCGGATGCTGGCCGTCTGTCCCCTGCGTGCC
GCTGCTGCCGGAGCGTGTGG

Downstream 100 bases:

>100_bases
GCGGCGCCGGCGCGGCCGTCGCGCGGCGTTGCGGGATATCCGGCACTCGGGTTGCCGCCGGCTACCCGAACCCTCGTCCG
CCGCATTGCCCGACCCGAAC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MSGTYLRVALDHPLATLFDYRCDVQPAPVPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSRLRAVDAICTELPPLSADWLA
LVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVRYRPNEAGRAALPDALPARGAALRRLAQALVDTGSLTLPDA
RALHPKAAATLDDWAARGWVDVEEIGWADAPVVNPVDNLSTGSGRAVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAEGERARNWLAAHTGRARIVLG
TRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAV
ADATLPTVRLIDLEEERRRGRASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
PEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALAEAVPGARILRIDADSTRRKGSAQALFSDVHAGEVDIL
VGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVMVQTRYPRHALYHALGRQDYVG
FANSTLAERRDAHLPPFVYQALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI

Sequences:

>Translated_753_residues
MSGTYLRVALDHPLATLFDYRCDVQPAPVPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSRLRAVDAICTELPPLSADWLA
LVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVRYRPNEAGRAALPDALPARGAALRRLAQALVDTGSLTLPDA
RALHPKAAATLDDWAARGWVDVEEIGWADAPVVNPVDNLSTGSGRAVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAEGERARNWLAAHTGRARIVLG
TRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAV
ADATLPTVRLIDLEEERRRGRASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
PEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALAEAVPGARILRIDADSTRRKGSAQALFSDVHAGEVDIL
VGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVMVQTRYPRHALYHALGRQDYVG
FANSTLAERRDAHLPPFVYQALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI
>Mature_752_residues
SGTYLRVALDHPLATLFDYRCDVQPAPVPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSRLRAVDAICTELPPLSADWLAL
VSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVRYRPNEAGRAALPDALPARGAALRRLAQALVDTGSLTLPDAR
ALHPKAAATLDDWAARGWVDVEEIGWADAPVVNPVDNLSTGSGRAVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSGK
TEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAEGERARNWLAAHTGRARIVLGT
RLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAVA
DATLPTVRLIDLEEERRRGRASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKP
EHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALAEAVPGARILRIDADSTRRKGSAQALFSDVHAGEVDILV
GTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVMVQTRYPRHALYHALGRQDYVGF
ANSTLAERRDAHLPPFVYQALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESASR
AALQHALRAWQPELRALKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=763, Percent_Identity=40.8912188728702, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81404; Mature: 81273

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTYLRVALDHPLATLFDYRCDVQPAPVPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSR
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCHHH
LRAVDAICTELPPLSADWLALVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHCCCCCCCCCCHHC
YRPNEAGRAALPDALPARGAALRRLAQALVDTGSLTLPDARALHPKAAATLDDWAARGWV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHHCCHHHHHHCCCC
DVEEIGWADAPVVNPVDNLSTGSGRAVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
CHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCCCC
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAE
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEHHCCCC
GERARNWLAAHTGRARIVLGTRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAV
CHHHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCHHHHHH
WRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAVADATLPTVRLIDLEEERRRG
HHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHCCCCCEEEEEECHHHHHCC
RASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
CHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
PEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALAEAVPGARILRIDADSTR
CCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
RKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFA
CCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHH
QLMQVSGRAGRAGLPGEVMVQTRYPRHALYHALGRQDYVGFANSTLAERRDAHLPPFVYQ
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHH
ALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI
HHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
SGTYLRVALDHPLATLFDYRCDVQPAPVPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSR
CCCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCHHH
LRAVDAICTELPPLSADWLALVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHCCCCCCCCCCHHC
YRPNEAGRAALPDALPARGAALRRLAQALVDTGSLTLPDARALHPKAAATLDDWAARGWV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCCHHHCCHHHHHHCCCC
DVEEIGWADAPVVNPVDNLSTGSGRAVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
CHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCCCC
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAE
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEHHCCCC
GERARNWLAAHTGRARIVLGTRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAV
CHHHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCHHHHHH
WRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAVADATLPTVRLIDLEEERRRG
HHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHCCCCCEEEEEECHHHHHCC
RASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
CHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
PEHALRCHHCGWEARIPRSCPECGNVDIAPLGRGTQRIEEALAEAVPGARILRIDADSTR
CCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
RKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFA
CCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHH
QLMQVSGRAGRAGLPGEVMVQTRYPRHALYHALGRQDYVGFANSTLAERRDAHLPPFVYQ
HHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHH
ALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI
HHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]