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The map label for this gene is paaF [C]

Identifier: 116626398

GI number: 116626398

Start: 9235442

End: 9236209

Strand: Direct

Name: paaF [C]

Synonym: Acid_7358

Alternate gene names: 116626398

Gene position: 9235442-9236209 (Clockwise)

Preceding gene: 116626397

Following gene: 116626401

Centisome position: 92.67

GC content: 65.23

Gene sequence:

>768_bases
ATGAACATCGAAATCGCCCACGAAGGTCCCATCGCGATCGTCACCTTGAACCGCCCCGAGCGCCGCAACGCGCTCTCGCT
CGAGCTGATGCTCGACCTGATCGCCGCGCTCGACGAGATCGGCCGCAATCGGGAGCTCCGCGCCGTGATCCTCGCCGCCG
CCGGCAAAGTCTTCTCCAGCGGCCACGATCTCTCCGAGATGACCGGGCGCGATATCAATGACTATCGCCGCATCTTCGAC
GTCTGCTGCGACCTGATGCAGAAAATCCAGTCGATTCCGCAGCCCGTGATCGCCGAGGTCCAGGGCATCGCCACCGCCGC
CGGCTGCCAGCTGGTCGCCACCTGTGACCTCGCCATTGCCGCGGATACTGCCCGTTTCGCCACCCCCGGTGTCAGGATCG
GCCTCTTCTGCACCACTCCGATGGTCGCCCTGACGCGCGCCATCGGCCGCAAGCGGGCCATGCAGATGCTGCTCACCGGC
GATTTCGTGGACGCGCCTACTGCCGCCGATTGGGGCCTCGTCAACCAGGTGGTTCCAGCGGCCGATCTGCAATCCGCCGC
GCGCGCCCTGGCGCAAAAAGTGGCCGCGGCCAGCTCCCTTGTGGTCGCCATCGGCAAGCAGGCCTTCTACACCCAAATCG
ACCTCGACCAGCCCAAGGCCTACGCCTACGCGAAGGAAGTCATGTCGATGAACGCCCTGGCCGCCGACGCCCAGGAAGGC
ATCTCTTCTTTCCTCGGCAAACGTGCTCCCTGCTGGAGTGGGAAATAG

Upstream 100 bases:

>100_bases
ACTTCAAGGCCCCCAAACTGGTGGAGTTCGGTCCGCTGCCGCGCACCGCCACCGGTAAGATCCGCAAGAACCAGCTGCGC
GCCCAGGCAAAGGCAAACTC

Downstream 100 bases:

>100_bases
GCTCGGCGCGTGGCGCGGTAGAATGTAAGAGGCACTGCAAATGTGTAATGTCACGCTATCCATACCGGATGAAATTCTGC
TCGCCTTAAAAGACGACGCC

Product: short chain enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MNIEIAHEGPIAIVTLNRPERRNALSLELMLDLIAALDEIGRNRELRAVILAAAGKVFSSGHDLSEMTGRDINDYRRIFD
VCCDLMQKIQSIPQPVIAEVQGIATAAGCQLVATCDLAIAADTARFATPGVRIGLFCTTPMVALTRAIGRKRAMQMLLTG
DFVDAPTAADWGLVNQVVPAADLQSAARALAQKVAAASSLVVAIGKQAFYTQIDLDQPKAYAYAKEVMSMNALAADAQEG
ISSFLGKRAPCWSGK

Sequences:

>Translated_255_residues
MNIEIAHEGPIAIVTLNRPERRNALSLELMLDLIAALDEIGRNRELRAVILAAAGKVFSSGHDLSEMTGRDINDYRRIFD
VCCDLMQKIQSIPQPVIAEVQGIATAAGCQLVATCDLAIAADTARFATPGVRIGLFCTTPMVALTRAIGRKRAMQMLLTG
DFVDAPTAADWGLVNQVVPAADLQSAARALAQKVAAASSLVVAIGKQAFYTQIDLDQPKAYAYAKEVMSMNALAADAQEG
ISSFLGKRAPCWSGK
>Mature_255_residues
MNIEIAHEGPIAIVTLNRPERRNALSLELMLDLIAALDEIGRNRELRAVILAAAGKVFSSGHDLSEMTGRDINDYRRIFD
VCCDLMQKIQSIPQPVIAEVQGIATAAGCQLVATCDLAIAADTARFATPGVRIGLFCTTPMVALTRAIGRKRAMQMLLTG
DFVDAPTAADWGLVNQVVPAADLQSAARALAQKVAAASSLVVAIGKQAFYTQIDLDQPKAYAYAKEVMSMNALAADAQEG
ISSFLGKRAPCWSGK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=241, Percent_Identity=46.4730290456432, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI194097323, Length=243, Percent_Identity=32.5102880658436, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI70995211, Length=186, Percent_Identity=31.1827956989247, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI62530384, Length=190, Percent_Identity=30.5263157894737, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI295842266, Length=215, Percent_Identity=29.7674418604651, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI157694516, Length=207, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI213417737, Length=207, Percent_Identity=26.0869565217391, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI4757968, Length=246, Percent_Identity=25.2032520325203, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=246, Percent_Identity=25.2032520325203, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI116235487, Length=206, Percent_Identity=27.1844660194175, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=30.859375, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787660, Length=276, Percent_Identity=30.7971014492754, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI87082183, Length=252, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=31.7269076305221, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI221142681, Length=260, Percent_Identity=29.2307692307692, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1788682, Length=195, Percent_Identity=30.7692307692308, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=42.3868312757202, Blast_Score=196, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17554946, Length=257, Percent_Identity=29.9610894941634, Blast_Score=129, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=31.7460317460317, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17560910, Length=251, Percent_Identity=25.8964143426295, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17535521, Length=218, Percent_Identity=28.4403669724771, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=203, Percent_Identity=28.5714285714286, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17540714, Length=203, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922422, Length=246, Percent_Identity=38.6178861788618, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI20129971, Length=254, Percent_Identity=32.6771653543307, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24653477, Length=254, Percent_Identity=32.6771653543307, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI19920382, Length=216, Percent_Identity=28.7037037037037, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=25.8964143426295, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24654903, Length=186, Percent_Identity=24.1935483870968, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21357171, Length=257, Percent_Identity=25.6809338521401, Blast_Score=71, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45550169, Length=176, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921018, Length=181, Percent_Identity=24.8618784530387, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27218; Mature: 27218

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEIAHEGPIAIVTLNRPERRNALSLELMLDLIAALDEIGRNRELRAVILAAAGKVFSS
CCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GHDLSEMTGRDINDYRRIFDVCCDLMQKIQSIPQPVIAEVQGIATAAGCQLVATCDLAIA
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEEHHC
ADTARFATPGVRIGLFCTTPMVALTRAIGRKRAMQMLLTGDFVDAPTAADWGLVNQVVPA
CCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCH
ADLQSAARALAQKVAAASSLVVAIGKQAFYTQIDLDQPKAYAYAKEVMSMNALAADAQEG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
ISSFLGKRAPCWSGK
HHHHHCCCCCCCCCC
>Mature Secondary Structure
MNIEIAHEGPIAIVTLNRPERRNALSLELMLDLIAALDEIGRNRELRAVILAAAGKVFSS
CCEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHC
GHDLSEMTGRDINDYRRIFDVCCDLMQKIQSIPQPVIAEVQGIATAAGCQLVATCDLAIA
CCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCEEEEEEEEHHC
ADTARFATPGVRIGLFCTTPMVALTRAIGRKRAMQMLLTGDFVDAPTAADWGLVNQVVPA
CCCHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCH
ADLQSAARALAQKVAAASSLVVAIGKQAFYTQIDLDQPKAYAYAKEVMSMNALAADAQEG
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
ISSFLGKRAPCWSGK
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA