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The map label for this gene is ntrC [H]

Identifier: 116626185

GI number: 116626185

Start: 8965783

End: 8967156

Strand: Direct

Name: ntrC [H]

Synonym: Acid_7145

Alternate gene names: 116626185

Gene position: 8965783-8967156 (Clockwise)

Preceding gene: 116626181

Following gene: 116626188

Centisome position: 89.97

GC content: 63.61

Gene sequence:

>1374_bases
ATGGGACCGACAGAAGTTTTATGGATCAGCGCGCAGCCGGAATTACCCGGCGACTGGCCCCACCATCCGGCGATGTGGAA
CCTGCAATTCCAAACCCCGGATGAGGCGCTGGAGAGCCTGGGCTCCACGGAGCACGCGGCCATCGTACTGGCATTGCCGG
TGAGCGGATGGAGCAGCTTCGAATTGCTGGATGCCGTCCAGCGCCGGGCCGCCGGAGTTCCCGTGTTGATCCGGGATGAA
ACGGTAACTCCCGGCGACGCGGTCCGGCTGGCGCACTTGGGCGTGTACCAGATTCTGACGACGGCAGGACAGGCGTTCGA
GCTGATCGACCAGGCGATCGAGGACTGGCGGCGCCGTCAGATGGCTAAGCTATCGGCCAGCCTGAGCGGGGCGGAGTGGA
CGCGTCAATTAATTGGCGGAAGCCGGGGAATGCGCCAACTGCATCATATTATTCATCTGGTGGCGGGCAGGCGCTCAACG
GTCCTGATCACGGGCGAGACCGGAACCGGCAAGGAACTCGCCGCGCGCGCGCTGCACCTGGGCGGGAAGCGCAGCTCGGG
ACCCATGGTGTCGGTCAACTGCCACGCCCTCCCGGAGAGTCTTCTGGAGAGCGAGTTGTTCGGTCATGTGCGCGGCGCGT
TCACCGGGGCCTTTCAGAATCGCATCGGACGTTTCGAGCAGGCCCAGGGCGGCACGCTCTTTCTGGACGAGATCGGCGAA
TTGCCGATCGATCTGCAAGCCAAGCTGCTGCGCGTGCTGCAGGAAAGAGAAGTACAGCGCTTAGGCAGTTCGGAGACGAT
CAAGGTTGATATTCGGGTGGTGGCGGCGACGAATTGCGACCTGCCGCGGCGCATCGAGGAGGGCAAGTTCCGCGAGGACC
TCTATTACCGGTTGAACGTGGTGCCGCTGGAAATGCCGCCCCTGCGCAGACGTCTCGAAGACGTTCCGATGTTGGCGGCG
CACTTCGTGGAGAAAGCGTGCCGCGCGGAGTCGATCCCGCCGAAGATGCTCACACGAGAGGCCCTCGAACTGCTGTGCGG
CTACTCCTGGCCAGGCAATGTAAGGCAGTTGGAGAACGTGGTGGAGATGGCGATTGCGCTCAGCGAAGACCGGCGCACAT
TGACCCCGATGGATTTCGTGCTGCCCGCAACCACGCGCAAGCCCGCCCCGGCCGCCCCCGGACCGTTGATCGCCTTGCCG
GCCGACGGCCTGGACTACGAACGGACGCTGGCCGTAATTGAACGCAGCATCCTCGAGCAGGCGCTGCGGCGAACGGGCGG
CAACAAAAAAGCAGCGGCGGATATGCTGCATTTGAAGCGAACCACGCTTTCTGCGAAGGTCCGGATTTTGGAACAGGCGG
CGGCCTGCTCGTGA

Upstream 100 bases:

>100_bases
CCAGCTTTGGCTGACTGACATATCGGCAGCTCGCGACAAAACCTGTAGGCAAGTGGACAACCTAAAGGTTCGGCAACACA
TTGCCGATGGATGAGTGGAT

Downstream 100 bases:

>100_bases
TAGTTACCAGATGTAATCTTTGGCGATATCGCGCTTGTCGTGGCCGTCCAGCACGTGGACCACGCGAATTCTCTTGAGCG
ACACCGGCGAGAGCTGCCCG

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MGPTEVLWISAQPELPGDWPHHPAMWNLQFQTPDEALESLGSTEHAAIVLALPVSGWSSFELLDAVQRRAAGVPVLIRDE
TVTPGDAVRLAHLGVYQILTTAGQAFELIDQAIEDWRRRQMAKLSASLSGAEWTRQLIGGSRGMRQLHHIIHLVAGRRST
VLITGETGTGKELAARALHLGGKRSSGPMVSVNCHALPESLLESELFGHVRGAFTGAFQNRIGRFEQAQGGTLFLDEIGE
LPIDLQAKLLRVLQEREVQRLGSSETIKVDIRVVAATNCDLPRRIEEGKFREDLYYRLNVVPLEMPPLRRRLEDVPMLAA
HFVEKACRAESIPPKMLTREALELLCGYSWPGNVRQLENVVEMAIALSEDRRTLTPMDFVLPATTRKPAPAAPGPLIALP
ADGLDYERTLAVIERSILEQALRRTGGNKKAAADMLHLKRTTLSAKVRILEQAAACS

Sequences:

>Translated_457_residues
MGPTEVLWISAQPELPGDWPHHPAMWNLQFQTPDEALESLGSTEHAAIVLALPVSGWSSFELLDAVQRRAAGVPVLIRDE
TVTPGDAVRLAHLGVYQILTTAGQAFELIDQAIEDWRRRQMAKLSASLSGAEWTRQLIGGSRGMRQLHHIIHLVAGRRST
VLITGETGTGKELAARALHLGGKRSSGPMVSVNCHALPESLLESELFGHVRGAFTGAFQNRIGRFEQAQGGTLFLDEIGE
LPIDLQAKLLRVLQEREVQRLGSSETIKVDIRVVAATNCDLPRRIEEGKFREDLYYRLNVVPLEMPPLRRRLEDVPMLAA
HFVEKACRAESIPPKMLTREALELLCGYSWPGNVRQLENVVEMAIALSEDRRTLTPMDFVLPATTRKPAPAAPGPLIALP
ADGLDYERTLAVIERSILEQALRRTGGNKKAAADMLHLKRTTLSAKVRILEQAAACS
>Mature_456_residues
GPTEVLWISAQPELPGDWPHHPAMWNLQFQTPDEALESLGSTEHAAIVLALPVSGWSSFELLDAVQRRAAGVPVLIRDET
VTPGDAVRLAHLGVYQILTTAGQAFELIDQAIEDWRRRQMAKLSASLSGAEWTRQLIGGSRGMRQLHHIIHLVAGRRSTV
LITGETGTGKELAARALHLGGKRSSGPMVSVNCHALPESLLESELFGHVRGAFTGAFQNRIGRFEQAQGGTLFLDEIGEL
PIDLQAKLLRVLQEREVQRLGSSETIKVDIRVVAATNCDLPRRIEEGKFREDLYYRLNVVPLEMPPLRRRLEDVPMLAAH
FVEKACRAESIPPKMLTREALELLCGYSWPGNVRQLENVVEMAIALSEDRRTLTPMDFVLPATTRKPAPAAPGPLIALPA
DGLDYERTLAVIERSILEQALRRTGGNKKAAADMLHLKRTTLSAKVRILEQAAACS

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=311, Percent_Identity=47.9099678456592, Blast_Score=275, Evalue=5e-75,
Organism=Escherichia coli, GI87082117, Length=321, Percent_Identity=48.2866043613707, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790299, Length=437, Percent_Identity=37.7574370709382, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788550, Length=333, Percent_Identity=44.4444444444444, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI1788905, Length=400, Percent_Identity=40, Blast_Score=267, Evalue=9e-73,
Organism=Escherichia coli, GI1789087, Length=324, Percent_Identity=46.6049382716049, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI87082152, Length=329, Percent_Identity=45.5927051671732, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1789233, Length=412, Percent_Identity=37.1359223300971, Blast_Score=239, Evalue=3e-64,
Organism=Escherichia coli, GI1786524, Length=325, Percent_Identity=44, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87081872, Length=323, Percent_Identity=40.5572755417957, Blast_Score=218, Evalue=5e-58,
Organism=Escherichia coli, GI1787583, Length=332, Percent_Identity=35.8433734939759, Blast_Score=190, Evalue=1e-49,
Organism=Escherichia coli, GI87081858, Length=359, Percent_Identity=29.8050139275766, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789828, Length=273, Percent_Identity=36.6300366300366, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50359; Mature: 50228

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPTEVLWISAQPELPGDWPHHPAMWNLQFQTPDEALESLGSTEHAAIVLALPVSGWSSF
CCCCEEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEECCCCCHH
ELLDAVQRRAAGVPVLIRDETVTPGDAVRLAHLGVYQILTTAGQAFELIDQAIEDWRRRQ
HHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKLSASLSGAEWTRQLIGGSRGMRQLHHIIHLVAGRRSTVLITGETGTGKELAARALHL
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHC
GGKRSSGPMVSVNCHALPESLLESELFGHVRGAFTGAFQNRIGRFEQAQGGTLFLDEIGE
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEHHHHCC
LPIDLQAKLLRVLQEREVQRLGSSETIKVDIRVVAATNCDLPRRIEEGKFREDLYYRLNV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHCCCHHHCCEEEEEE
VPLEMPPLRRRLEDVPMLAAHFVEKACRAESIPPKMLTREALELLCGYSWPGNVRQLENV
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VEMAIALSEDRRTLTPMDFVLPATTRKPAPAAPGPLIALPADGLDYERTLAVIERSILEQ
HHHHHHHCCCCCCCCCHHHEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
ALRRTGGNKKAAADMLHLKRTTLSAKVRILEQAAACS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GPTEVLWISAQPELPGDWPHHPAMWNLQFQTPDEALESLGSTEHAAIVLALPVSGWSSF
CCCEEEEEECCCCCCCCCCCCCCEEEEEECCHHHHHHHCCCCCCEEEEEEEECCCCCHH
ELLDAVQRRAAGVPVLIRDETVTPGDAVRLAHLGVYQILTTAGQAFELIDQAIEDWRRRQ
HHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKLSASLSGAEWTRQLIGGSRGMRQLHHIIHLVAGRRSTVLITGETGTGKELAARALHL
HHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHC
GGKRSSGPMVSVNCHALPESLLESELFGHVRGAFTGAFQNRIGRFEQAQGGTLFLDEIGE
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEHHHHCC
LPIDLQAKLLRVLQEREVQRLGSSETIKVDIRVVAATNCDLPRRIEEGKFREDLYYRLNV
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHHCCCHHHCCEEEEEE
VPLEMPPLRRRLEDVPMLAAHFVEKACRAESIPPKMLTREALELLCGYSWPGNVRQLENV
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHH
VEMAIALSEDRRTLTPMDFVLPATTRKPAPAAPGPLIALPADGLDYERTLAVIERSILEQ
HHHHHHHCCCCCCCCCHHHEECCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHH
ALRRTGGNKKAAADMLHLKRTTLSAKVRILEQAAACS
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2881918; 11481430; 8478331 [H]