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The map label for this gene is proS [H]

Identifier: 116626061

GI number: 116626061

Start: 8822435

End: 8823877

Strand: Direct

Name: proS [H]

Synonym: Acid_7019

Alternate gene names: 116626061

Gene position: 8822435-8823877 (Clockwise)

Preceding gene: 116626056

Following gene: 116626063

Centisome position: 88.53

GC content: 67.15

Gene sequence:

>1443_bases
ATGTTAGAATTCAGGCCTATGCGGTGGAGCAGGCTATTCATTCCGACGCTGCGCGAAAATCCGGCAGACGCGGAAACGGT
GAGCCAGCAGCTGCTGGTGCGCGCGGGCTACGCGCGCGCGGTGAAGACCGGAGTCTACGGGTGGCTGCCGTTGGGGCAGC
GAGCGATGGCGAAGATTCAGCGCATCGCGCGGCAGGAATTGACGGCGCTCGGCGGGCAGGAAGTAGGGCTGGACGCAGCG
GATGCGGCGGAGATCGGGCGCGGCGAGATTCGCGGCGCGAAATCGCTGCCGCAGATCTGGTTTCGCGTGGAGGCTCCGCT
GTTGCGGAGGCGGCAGTTCGCGGGACTGGACGTCTACGGGTTCGGCGAGGACGCGGGTGCAATGCGCGAGTGCTTCCAGC
GTATTCTGGATCGCTGCGGCGTGAGCGCGCTGGAGGTGGAAGACGCGTTCTTCGCAGTGATGGAGGCGGGGACGGACACG
GTGGTGGTGTGCCGGGGCTGCGGATATGCGGCGAGTCCCCGGACGGCGTGGAGCGCGGCGTCGGCGCCTGGAATCGCCGA
TCCGGAGGGCGACCTGACACCCGAGGAGTTCCACACACCAGGGCAGAAGACGATCGCCGACATCGCGAGTTTCACGGGGC
TGCCGGAGAGCGCGCAGATGAAGAGCCTGGTGCTGGTGGCGAACCAGAAGCCGGTGCTGGTGATGCTGCGCGGCGACCAT
CAGCTCAATCCGGCGCGTTTCGCGGCGAAGACGGGCGACGCGGGATTCCGCCAGGCGCTGCCGGAGGAGTTATTCCGGTG
GTTCGGCGCGCAGGCCGGTTCGCTGGGGCCGGTCGGAGTGAAGGACATGCCGGTGCTGGCGGATTCGGCGCTTGCGGGGC
GGCGGAACATGATCAGCGGCGCAAACCGCACCGACTATCATTTGCGCCACGTGACGCCGGGCGAGGATTTCGCGGCGGAG
TTCTGCGATTTGCGCGAGGCGGCGGCAGGCGATGCGTGCGCGCGCTGCGGCGCGGTGCTGGAATTTCGCAACGCCGTGGA
ACTGGCGCGGATGACTGCGGGCCTATACCGGCTGGCATTGGATCGGATTTTGACGACCGCGATTGAAGCGGGCAACGACA
AGGACGGCATGGCACTGCCGGCCGGCGTGGCGCCGTTCGACGTGGTGGTGTCGGTGGCGGTGGCGGGCGATGCGGCGCAG
ATGGAGGCGGCGGAGCGGATCTACGCGGAGTGTACGGCGGCCGGGATGGACCTGCTGTTCGACGATCGCGATGAGCGTCC
AGGGGTGAAATTCAAGGACGCGGATTTGATCGGCGTGCCGTGGCGGGTGACGGTCGGCAAGAAAATCGCGGGCGGCCTGG
TGGAATTGGTGGAGCGCAAGACGAAGGTGGTGACGGCTGTGCCGATTGCGAGCGTGATGGAGGCTATCGACCAACCGCGT
TGA

Upstream 100 bases:

>100_bases
CCGTCCTGAGCCAGTTTCTGCCGCAGTTTTTCCTTGAGAGAAAACCCGGCGTGATCCGCGCCAATGGCGATTTTCATAAG
GGGAGAATCATGATTTTAAC

Downstream 100 bases:

>100_bases
CTCTGACGATGGCGACGCGGCCGACGGAGGCCATCGCGTTGCATCGGGGCGAGCCGGCTGAAAGCCGGCTGCAGGCAGGA
TTGCCTGCCCCACTTCTGAC

Product: anticodon-binding domain-containing protein

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MLEFRPMRWSRLFIPTLRENPADAETVSQQLLVRAGYARAVKTGVYGWLPLGQRAMAKIQRIARQELTALGGQEVGLDAA
DAAEIGRGEIRGAKSLPQIWFRVEAPLLRRRQFAGLDVYGFGEDAGAMRECFQRILDRCGVSALEVEDAFFAVMEAGTDT
VVVCRGCGYAASPRTAWSAASAPGIADPEGDLTPEEFHTPGQKTIADIASFTGLPESAQMKSLVLVANQKPVLVMLRGDH
QLNPARFAAKTGDAGFRQALPEELFRWFGAQAGSLGPVGVKDMPVLADSALAGRRNMISGANRTDYHLRHVTPGEDFAAE
FCDLREAAAGDACARCGAVLEFRNAVELARMTAGLYRLALDRILTTAIEAGNDKDGMALPAGVAPFDVVVSVAVAGDAAQ
MEAAERIYAECTAAGMDLLFDDRDERPGVKFKDADLIGVPWRVTVGKKIAGGLVELVERKTKVVTAVPIASVMEAIDQPR

Sequences:

>Translated_480_residues
MLEFRPMRWSRLFIPTLRENPADAETVSQQLLVRAGYARAVKTGVYGWLPLGQRAMAKIQRIARQELTALGGQEVGLDAA
DAAEIGRGEIRGAKSLPQIWFRVEAPLLRRRQFAGLDVYGFGEDAGAMRECFQRILDRCGVSALEVEDAFFAVMEAGTDT
VVVCRGCGYAASPRTAWSAASAPGIADPEGDLTPEEFHTPGQKTIADIASFTGLPESAQMKSLVLVANQKPVLVMLRGDH
QLNPARFAAKTGDAGFRQALPEELFRWFGAQAGSLGPVGVKDMPVLADSALAGRRNMISGANRTDYHLRHVTPGEDFAAE
FCDLREAAAGDACARCGAVLEFRNAVELARMTAGLYRLALDRILTTAIEAGNDKDGMALPAGVAPFDVVVSVAVAGDAAQ
MEAAERIYAECTAAGMDLLFDDRDERPGVKFKDADLIGVPWRVTVGKKIAGGLVELVERKTKVVTAVPIASVMEAIDQPR
>Mature_480_residues
MLEFRPMRWSRLFIPTLRENPADAETVSQQLLVRAGYARAVKTGVYGWLPLGQRAMAKIQRIARQELTALGGQEVGLDAA
DAAEIGRGEIRGAKSLPQIWFRVEAPLLRRRQFAGLDVYGFGEDAGAMRECFQRILDRCGVSALEVEDAFFAVMEAGTDT
VVVCRGCGYAASPRTAWSAASAPGIADPEGDLTPEEFHTPGQKTIADIASFTGLPESAQMKSLVLVANQKPVLVMLRGDH
QLNPARFAAKTGDAGFRQALPEELFRWFGAQAGSLGPVGVKDMPVLADSALAGRRNMISGANRTDYHLRHVTPGEDFAAE
FCDLREAAAGDACARCGAVLEFRNAVELARMTAGLYRLALDRILTTAIEAGNDKDGMALPAGVAPFDVVVSVAVAGDAAQ
MEAAERIYAECTAAGMDLLFDDRDERPGVKFKDADLIGVPWRVTVGKKIAGGLVELVERKTKVVTAVPIASVMEAIDQPR

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786392, Length=543, Percent_Identity=27.8084714548803, Blast_Score=187, Evalue=2e-48,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 51593; Mature: 51593

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFRPMRWSRLFIPTLRENPADAETVSQQLLVRAGYARAVKTGVYGWLPLGQRAMAKIQ
CCCCCCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RIARQELTALGGQEVGLDAADAAEIGRGEIRGAKSLPQIWFRVEAPLLRRRQFAGLDVYG
HHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCEEEE
FGEDAGAMRECFQRILDRCGVSALEVEDAFFAVMEAGTDTVVVCRGCGYAASPRTAWSAA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
SAPGIADPEGDLTPEEFHTPGQKTIADIASFTGLPESAQMKSLVLVANQKPVLVMLRGDH
CCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHCEEEEEECCCCEEEEEECCC
QLNPARFAAKTGDAGFRQALPEELFRWFGAQAGSLGPVGVKDMPVLADSALAGRRNMISG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHCCC
ANRTDYHLRHVTPGEDFAAEFCDLREAAAGDACARCGAVLEFRNAVELARMTAGLYRLAL
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRILTTAIEAGNDKDGMALPAGVAPFDVVVSVAVAGDAAQMEAAERIYAECTAAGMDLLF
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEE
DDRDERPGVKFKDADLIGVPWRVTVGKKIAGGLVELVERKTKVVTAVPIASVMEAIDQPR
CCCCCCCCCEECCCCEECCCEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLEFRPMRWSRLFIPTLRENPADAETVSQQLLVRAGYARAVKTGVYGWLPLGQRAMAKIQ
CCCCCCCCCCEEECCHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RIARQELTALGGQEVGLDAADAAEIGRGEIRGAKSLPQIWFRVEAPLLRRRQFAGLDVYG
HHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCEEEE
FGEDAGAMRECFQRILDRCGVSALEVEDAFFAVMEAGTDTVVVCRGCGYAASPRTAWSAA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
SAPGIADPEGDLTPEEFHTPGQKTIADIASFTGLPESAQMKSLVLVANQKPVLVMLRGDH
CCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHCCCCCCHHHCEEEEEECCCCEEEEEECCC
QLNPARFAAKTGDAGFRQALPEELFRWFGAQAGSLGPVGVKDMPVLADSALAGRRNMISG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCHHHCCC
ANRTDYHLRHVTPGEDFAAEFCDLREAAAGDACARCGAVLEFRNAVELARMTAGLYRLAL
CCCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRILTTAIEAGNDKDGMALPAGVAPFDVVVSVAVAGDAAQMEAAERIYAECTAAGMDLLF
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHEE
DDRDERPGVKFKDADLIGVPWRVTVGKKIAGGLVELVERKTKVVTAVPIASVMEAIDQPR
CCCCCCCCCEECCCCEECCCEEEECCHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA