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The map label for this gene is rfbB [H]

Identifier: 116625985

GI number: 116625985

Start: 8725396

End: 8726145

Strand: Reverse

Name: rfbB [H]

Synonym: Acid_6943

Alternate gene names: 116625985

Gene position: 8726145-8725396 (Counterclockwise)

Preceding gene: 116625986

Following gene: 116625984

Centisome position: 87.56

GC content: 56.93

Gene sequence:

>750_bases
ATGACAGATATCTACCTTCGCTTGCGCGGTGTGGGGATGACATTTGAAGGGCAGATCGGCGGCCAGGACGGTTTGCAGTC
CTGGCTTCCCCGGTGCTTTGCCAAAGAACCCGCGGGGCCGCAGCGCTACCGGGTCGAGGCCCTGAACGACATTAACCTGG
AAATCAAACACGGTGAGCGAGTCGGGTTGATAGGTCTCAACGGAGCAGGGAAGAGTACGCTTCTGAAAGTTCTGGCCGGC
ATCTATCCACCAACGGCGGGTCACATCGAAAAGCGCGGCCATGTCTGCCCGATGTTCGAGTTCGCAACGGGTTTTGAAAT
GAATCAATCCGGTTGGGACAATATCCGCATCCGAGGCCTGCTGCTGGGTATGGCCCCGGCCGAGATTGAAGCCAAACTGC
ACGAAATCGGCGAATTCACGGAGTTGGGCGAGTTTCTCTCGTATCCCGTGCGGACCTATTCCTCCGGCATGTTCATCCGC
CTGGCATTCGCGGTTTCCACGAGCATCAACCCCGAAATCCTGTTGCTTGACGAGGTGATGGCGGCGGGAGACATCCGCTT
CGCCGAAAAGGCCAGAAAGAGGATGTTCGAATTCATGGAGCAGGGCAAAATATTGGTATTCTGCTCGCATAACGCCGAAC
TTCTCGGCACCTTCTGTGAAAGGACGATCTTGTTGGACAAAGGCAAGGTGGTCGCTGATGGCCCTACCGCTGAGATCACT
GCGCGGTATCGGTGCATGGCCGCGCCGTAG

Upstream 100 bases:

>100_bases
CTCCGGAGATCTATGCGTTCGCGGGAGTCTATATTCTCCTGGTCTGGGCCTTCGCCGGTCTGGTTGCCAGGTCATTGGAT
TCGCGAGTTGTGCTTCTGCT

Downstream 100 bases:

>100_bases
TGTTTGGCAGCGCTCGAATGGTTGGAAGCGCTTTGAAGCGAGAGATAAGGAGATTTTGTACTTGATTCGTGCCGAGTATT
TCATGCAGGAGGCGAAGGTT

Product: ABC transporter-like protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTDIYLRLRGVGMTFEGQIGGQDGLQSWLPRCFAKEPAGPQRYRVEALNDINLEIKHGERVGLIGLNGAGKSTLLKVLAG
IYPPTAGHIEKRGHVCPMFEFATGFEMNQSGWDNIRIRGLLLGMAPAEIEAKLHEIGEFTELGEFLSYPVRTYSSGMFIR
LAFAVSTSINPEILLLDEVMAAGDIRFAEKARKRMFEFMEQGKILVFCSHNAELLGTFCERTILLDKGKVVADGPTAEIT
ARYRCMAAP

Sequences:

>Translated_249_residues
MTDIYLRLRGVGMTFEGQIGGQDGLQSWLPRCFAKEPAGPQRYRVEALNDINLEIKHGERVGLIGLNGAGKSTLLKVLAG
IYPPTAGHIEKRGHVCPMFEFATGFEMNQSGWDNIRIRGLLLGMAPAEIEAKLHEIGEFTELGEFLSYPVRTYSSGMFIR
LAFAVSTSINPEILLLDEVMAAGDIRFAEKARKRMFEFMEQGKILVFCSHNAELLGTFCERTILLDKGKVVADGPTAEIT
ARYRCMAAP
>Mature_248_residues
TDIYLRLRGVGMTFEGQIGGQDGLQSWLPRCFAKEPAGPQRYRVEALNDINLEIKHGERVGLIGLNGAGKSTLLKVLAGI
YPPTAGHIEKRGHVCPMFEFATGFEMNQSGWDNIRIRGLLLGMAPAEIEAKLHEIGEFTELGEFLSYPVRTYSSGMFIRL
AFAVSTSINPEILLLDEVMAAGDIRFAEKARKRMFEFMEQGKILVFCSHNAELLGTFCERTILLDKGKVVADGPTAEITA
RYRCMAAP

Specific function: May form an ATP-driven O-antigen export apparatus, in association with RfbA [H]

COG id: COG1134

COG function: function code GM; ABC-type polysaccharide/polyol phosphate transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI27477115, Length=228, Percent_Identity=25.4385964912281, Blast_Score=81, Evalue=8e-16,
Organism=Homo sapiens, GI6005701, Length=228, Percent_Identity=27.1929824561404, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI153792144, Length=219, Percent_Identity=25.1141552511416, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI150417984, Length=221, Percent_Identity=27.6018099547511, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI31657092, Length=212, Percent_Identity=27.8301886792453, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI116734710, Length=215, Percent_Identity=25.5813953488372, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI30795238, Length=215, Percent_Identity=26.046511627907, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI27881501, Length=213, Percent_Identity=26.2910798122066, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI27436953, Length=230, Percent_Identity=25.2173913043478, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI47078218, Length=205, Percent_Identity=27.3170731707317, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI45446740, Length=205, Percent_Identity=27.3170731707317, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788761, Length=213, Percent_Identity=30.9859154929577, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081791, Length=214, Percent_Identity=32.7102803738318, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994943, Length=211, Percent_Identity=27.4881516587678, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790467, Length=202, Percent_Identity=26.7326732673267, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787758, Length=246, Percent_Identity=26.4227642276423, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87081709, Length=205, Percent_Identity=25.8536585365854, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789751, Length=184, Percent_Identity=32.6086956521739, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788225, Length=211, Percent_Identity=27.4881516587678, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787370, Length=221, Percent_Identity=26.6968325791855, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87081782, Length=211, Percent_Identity=25.5924170616114, Blast_Score=67, Evalue=9e-13,
Organism=Escherichia coli, GI48995001, Length=216, Percent_Identity=25.462962962963, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1786703, Length=213, Percent_Identity=26.7605633802817, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787029, Length=208, Percent_Identity=25.9615384615385, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789593, Length=210, Percent_Identity=25.7142857142857, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788210, Length=252, Percent_Identity=23.4126984126984, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=217, Percent_Identity=25.3456221198157, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17510237, Length=212, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=218, Percent_Identity=26.605504587156, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=214, Percent_Identity=25.2336448598131, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71983510, Length=214, Percent_Identity=25.2336448598131, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI71983516, Length=214, Percent_Identity=25.2336448598131, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24666092, Length=214, Percent_Identity=24.2990654205607, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI116007184, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI221500365, Length=193, Percent_Identity=27.979274611399, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573571, Length=211, Percent_Identity=25.1184834123223, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573573, Length=211, Percent_Identity=25.1184834123223, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221512771, Length=220, Percent_Identity=25, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: 3.6.3.25 [C]

Molecular weight: Translated: 27576; Mature: 27445

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDIYLRLRGVGMTFEGQIGGQDGLQSWLPRCFAKEPAGPQRYRVEALNDINLEIKHGER
CCEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCE
VGLIGLNGAGKSTLLKVLAGIYPPTAGHIEKRGHVCPMFEFATGFEMNQSGWDNIRIRGL
EEEEEECCCCHHHHHHHHHHCCCCCCCCHHCCCCCCEEHHHHCCCCCCCCCCCCEEEEEE
LLGMAPAEIEAKLHEIGEFTELGEFLSYPVRTYSSGMFIRLAFAVSTSINPEILLLDEVM
EEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEEEEEECCCCCCEEEEEHHH
AAGDIRFAEKARKRMFEFMEQGKILVFCSHNAELLGTFCERTILLDKGKVVADGPTAEIT
HHCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHEEEEECCEEEECCCCCEEE
ARYRCMAAP
EEEEEEECC
>Mature Secondary Structure 
TDIYLRLRGVGMTFEGQIGGQDGLQSWLPRCFAKEPAGPQRYRVEALNDINLEIKHGER
CEEEEEEEECCEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEECCCE
VGLIGLNGAGKSTLLKVLAGIYPPTAGHIEKRGHVCPMFEFATGFEMNQSGWDNIRIRGL
EEEEEECCCCHHHHHHHHHHCCCCCCCCHHCCCCCCEEHHHHCCCCCCCCCCCCEEEEEE
LLGMAPAEIEAKLHEIGEFTELGEFLSYPVRTYSSGMFIRLAFAVSTSINPEILLLDEVM
EEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEEEEEECCCCCCEEEEEHHH
AAGDIRFAEKARKRMFEFMEQGKILVFCSHNAELLGTFCERTILLDKGKVVADGPTAEIT
HHCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHEEEEECCEEEECCCCCEEE
ARYRCMAAP
EEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8626291 [H]