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The map label for this gene is dgoD [H]

Identifier: 116625817

GI number: 116625817

Start: 8483222

End: 8484466

Strand: Reverse

Name: dgoD [H]

Synonym: Acid_6768

Alternate gene names: 116625817

Gene position: 8484466-8483222 (Counterclockwise)

Preceding gene: 116625818

Following gene: 116625816

Centisome position: 85.14

GC content: 58.63

Gene sequence:

>1245_bases
ATGGGTTCATTGAACAGAAGGTCATTCTTCCGCGCCGGAGCGGGCGTAGCCGGTGCATTCGCCATGGAGAATCTGTCCGC
CGCAGTGCAGGACAATAGTACGCCGTACCAGAGGCCGAAGCTGAAAATTACGGACGTAAAAGCTGCCAACATCCAGGGTT
TCCATGTCCGCATCTTCACCGATCAGGGTTTGTACGGCGATGGCGAAGGCGTTGACGCGGTGAGCGGCGGACCCTCGATC
GTGCAGGGCTTTCGCTCTTCTTTAATCGGTCAAAGCCCCCTAAACATCGAGGGTATCTGGGAGCGCATCCGCACCAGCGG
CGTTTTCGCGGGCGCTCAGGGCGGCCAGTACGTGGCGACCTTGACCGCCGTGGAAATCGCCTTGTGGGACCTGGCTGGGA
AAGCCCTCAACATGCCGATTTACCAACTCATGGGCGGCAAGGTACGCGACAAGATTCGAGTATATTGCGACTCCGGCACC
GACTATCGTGACGATCCGCAGGCAAAGCCGTATATCAAGCAGATTATCGATAACGGCTTCACCATGGCCAAGATCGATAT
CGACGACTCCGCGGATCCCGCGCGCTTCGATCGCGTGAACTGGACCGCCAACAACGCTGAAATCGATCATATGGTCGACA
AGGTTGCGTTCATGCGCGAGTCCTTGCCGAAATCGATCGAATTGGCGGTGGATATGCACGGCAGATACGATCTGCCGACG
GCGAAACGAGTTGCCAAAGAGCTCGAACCGTTCAAGCTCGTATGGCTTGAAGAGCCGGTACCGGCGGGAAATCTCGACGC
CATGCGGGACGTGCGGCAATCGACGCACACTCCGATCTGCGCCGGTGAGAACATCTACCTGCGCTGGGGGTTCCGGGAAG
TATTCGAGAAACAGGCTCTCGACATCATCATGCCCGATATTCAGAAGTGCGGCGGACTGATGGAGGGGAAGAAGATCGCC
GACATGGCGCAGGCCTACTATATTCCGATCGCGCCGCATTCCCAGGCCTCGCCCATCGGCGCCATGGCGACCGCTCACAT
GCTGGCGACCGTGCCCAACTTCCTGGTTGTCGAGTGGCACTGGAGCCACCCGGCCCAGCGCTGGGAGCGGTGGAAGCAGT
TCGTGAAGGGAGAAGACATCATCCAGAAGGGCTACATTACGGTGCCGGACAAGCCCGGCATCGGCGTGGAAATGAATGAG
GACTACCTCAAGAAGCTACCGGGAGCGACCTGGTTTCCGGCATAG

Upstream 100 bases:

>100_bases
AAGTGGCAAGCACAGGTCAATTCGGCGCGCCAAATGCAGTTGGTGGCGAAACTGACCTGGTAAGGTTTCGCGCAGTTGTG
AGAATCTGCAGGGAGCATCA

Downstream 100 bases:

>100_bases
ACCACAGGCAACGGCAAAACGACAATCGAGGTCGATATGAGAAGTTGGCCCGATGGGCATCACATCGCCAAGAACATGTT
CGCCATCGCCTTCTGCATCG

Product: mandelate racemase/muconate lactonizing protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGSLNRRSFFRAGAGVAGAFAMENLSAAVQDNSTPYQRPKLKITDVKAANIQGFHVRIFTDQGLYGDGEGVDAVSGGPSI
VQGFRSSLIGQSPLNIEGIWERIRTSGVFAGAQGGQYVATLTAVEIALWDLAGKALNMPIYQLMGGKVRDKIRVYCDSGT
DYRDDPQAKPYIKQIIDNGFTMAKIDIDDSADPARFDRVNWTANNAEIDHMVDKVAFMRESLPKSIELAVDMHGRYDLPT
AKRVAKELEPFKLVWLEEPVPAGNLDAMRDVRQSTHTPICAGENIYLRWGFREVFEKQALDIIMPDIQKCGGLMEGKKIA
DMAQAYYIPIAPHSQASPIGAMATAHMLATVPNFLVVEWHWSHPAQRWERWKQFVKGEDIIQKGYITVPDKPGIGVEMNE
DYLKKLPGATWFPA

Sequences:

>Translated_414_residues
MGSLNRRSFFRAGAGVAGAFAMENLSAAVQDNSTPYQRPKLKITDVKAANIQGFHVRIFTDQGLYGDGEGVDAVSGGPSI
VQGFRSSLIGQSPLNIEGIWERIRTSGVFAGAQGGQYVATLTAVEIALWDLAGKALNMPIYQLMGGKVRDKIRVYCDSGT
DYRDDPQAKPYIKQIIDNGFTMAKIDIDDSADPARFDRVNWTANNAEIDHMVDKVAFMRESLPKSIELAVDMHGRYDLPT
AKRVAKELEPFKLVWLEEPVPAGNLDAMRDVRQSTHTPICAGENIYLRWGFREVFEKQALDIIMPDIQKCGGLMEGKKIA
DMAQAYYIPIAPHSQASPIGAMATAHMLATVPNFLVVEWHWSHPAQRWERWKQFVKGEDIIQKGYITVPDKPGIGVEMNE
DYLKKLPGATWFPA
>Mature_413_residues
GSLNRRSFFRAGAGVAGAFAMENLSAAVQDNSTPYQRPKLKITDVKAANIQGFHVRIFTDQGLYGDGEGVDAVSGGPSIV
QGFRSSLIGQSPLNIEGIWERIRTSGVFAGAQGGQYVATLTAVEIALWDLAGKALNMPIYQLMGGKVRDKIRVYCDSGTD
YRDDPQAKPYIKQIIDNGFTMAKIDIDDSADPARFDRVNWTANNAEIDHMVDKVAFMRESLPKSIELAVDMHGRYDLPTA
KRVAKELEPFKLVWLEEPVPAGNLDAMRDVRQSTHTPICAGENIYLRWGFREVFEKQALDIIMPDIQKCGGLMEGKKIAD
MAQAYYIPIAPHSQASPIGAMATAHMLATVPNFLVVEWHWSHPAQRWERWKQFVKGEDIIQKGYITVPDKPGIGVEMNED
YLKKLPGATWFPA

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=368, Percent_Identity=32.6086956521739, Blast_Score=182, Evalue=2e-47,
Organism=Escherichia coli, GI1787864, Length=375, Percent_Identity=27.4666666666667, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI226510960, Length=375, Percent_Identity=26.1333333333333, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 45964; Mature: 45833

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSLNRRSFFRAGAGVAGAFAMENLSAAVQDNSTPYQRPKLKITDVKAANIQGFHVRIFT
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEEEEEEEE
DQGLYGDGEGVDAVSGGPSIVQGFRSSLIGQSPLNIEGIWERIRTSGVFAGAQGGQYVAT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEECCCCCCEEEH
LTAVEIALWDLAGKALNMPIYQLMGGKVRDKIRVYCDSGTDYRDDPQAKPYIKQIIDNGF
HHHHHHHHHHHCCHHHCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCC
TMAKIDIDDSADPARFDRVNWTANNAEIDHMVDKVAFMRESLPKSIELAVDMHGRYDLPT
EEEEEECCCCCCCCHHCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCH
AKRVAKELEPFKLVWLEEPVPAGNLDAMRDVRQSTHTPICAGENIYLRWGFREVFEKQAL
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECHHHHHHHHHH
DIIMPDIQKCGGLMEGKKIADMAQAYYIPIAPHSQASPIGAMATAHMLATVPNFLVVEWH
HHHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEE
WSHPAQRWERWKQFVKGEDIIQKGYITVPDKPGIGVEMNEDYLKKLPGATWFPA
CCCHHHHHHHHHHHHCCHHHHHCCCEECCCCCCCCCEECHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GSLNRRSFFRAGAGVAGAFAMENLSAAVQDNSTPYQRPKLKITDVKAANIQGFHVRIFT
CCCCCHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCEEEEEEEE
DQGLYGDGEGVDAVSGGPSIVQGFRSSLIGQSPLNIEGIWERIRTSGVFAGAQGGQYVAT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCEEECCCCCCEEEH
LTAVEIALWDLAGKALNMPIYQLMGGKVRDKIRVYCDSGTDYRDDPQAKPYIKQIIDNGF
HHHHHHHHHHHCCHHHCCCHHHHCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHCCC
TMAKIDIDDSADPARFDRVNWTANNAEIDHMVDKVAFMRESLPKSIELAVDMHGRYDLPT
EEEEEECCCCCCCCHHCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCH
AKRVAKELEPFKLVWLEEPVPAGNLDAMRDVRQSTHTPICAGENIYLRWGFREVFEKQAL
HHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCEECCCEEEEEECHHHHHHHHHH
DIIMPDIQKCGGLMEGKKIADMAQAYYIPIAPHSQASPIGAMATAHMLATVPNFLVVEWH
HHHHHHHHHHCCCCCCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHCCCEEEEEEE
WSHPAQRWERWKQFVKGEDIIQKGYITVPDKPGIGVEMNEDYLKKLPGATWFPA
CCCHHHHHHHHHHHHCCHHHHHCCCEECCCCCCCCCEECHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA