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The map label for this gene is psdht [H]

Identifier: 116625716

GI number: 116625716

Start: 8368614

End: 8369555

Strand: Direct

Name: psdht [H]

Synonym: Acid_6665

Alternate gene names: 116625716

Gene position: 8368614-8369555 (Clockwise)

Preceding gene: 116625715

Following gene: 116625725

Centisome position: 83.97

GC content: 64.54

Gene sequence:

>942_bases
ATGAATCTCGACATCCTGGGAATGCGCGAAGGAATTTCGCGAACCTACTCCGTCATTCGCCCGTTCATCCGCCGCACTCC
CGTAGTCGATATCGACGCCGGCGATTTCGCGCTTCCCGCCGCGCCCCTCGTCCTCAAACTGGAACTACTCCAACACACGG
GATCCTTCAAAACGCGCGGCGCCGTCTCCAATCTTCTGACCCGAACAGTGCCGCCCGCCGGCGTGGTAGCCGCATCCGGA
GGCAACCACGGAGTCGCCGTCGCCTACGCCGCGATGAAGGCCGGCAAACCCGCACGCATTTTCATCCCGACGATTTCTTC
CCCGGCGAAAATTGATCGCATCCGCTCCTACGGCGCCGACCTAGTCGTCACCGGCGACCGTTACCACGATGCGCTCCTGG
CCAGCCAGGAGTGGGCCGCGCAATCAGGCGCCATGCAGATCCACGCGTATGACCAGGTGGAGACGCTCCTCGGTGCAGGT
TCGGTCGGGTTGGAATTCGAGGAACAGGCACCCGCCCTCGATACCCTCCTCGTCGCGTGCGGCGGAGGCGGACTAATTGG
CGGTATCGCCGGCTGGTTTCAGGGGCGCACTCGCGTCGTCGGCGTCGAGCCTGAGTTATGCCCCACTCTTCACCGCGCAC
TCGAGGCTGGACGCCCCGTCGATACGGAATCCAGCGGCATCGCCGCCGATTCGCTGGCCCCCAACAGCGTCGGCGCCCTC
ATGTTCCCCATCGCCCAAAAGTGGGTAGAACGCGTCGTCCTGGTTCCCGACAACGCTATCGCCGACGCCCAGCGCGCCCT
CTGGAATACCCTTCGAGTCGTTACCGAACCCGGCGGCGCCGCTGCATTCGCCGCCGTTCTCTCCCGCGCATACGAACCTG
CGGCGGGAGAGCGCGTCGGCGTTCTGCTATGCGGCGCCAACACCACTGCCGTCAATTTCTAA

Upstream 100 bases:

>100_bases
GCCCGTCTTTTTCGGCTTCGGCATCATGCCTCTCATCTGCGCCACCATCCTGTGGACGCTGATGGGGCCGCTCCGGCCCG
CCCGTCTATAATACGAGTTC

Downstream 100 bases:

>100_bases
CTATTGCTTGGAGCGGTCGGCACTCGTCGACGTGCCTTTGCTGGTACTTTTGCTGATATCCAGGATCATTCGTTGATGCT
CCTGCAGCGTGGGCAGCGTC

Product: threonine dehydratase

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLDILGMREGISRTYSVIRPFIRRTPVVDIDAGDFALPAAPLVLKLELLQHTGSFKTRGAVSNLLTRTVPPAGVVAASG
GNHGVAVAYAAMKAGKPARIFIPTISSPAKIDRIRSYGADLVVTGDRYHDALLASQEWAAQSGAMQIHAYDQVETLLGAG
SVGLEFEEQAPALDTLLVACGGGGLIGGIAGWFQGRTRVVGVEPELCPTLHRALEAGRPVDTESSGIAADSLAPNSVGAL
MFPIAQKWVERVVLVPDNAIADAQRALWNTLRVVTEPGGAAAFAAVLSRAYEPAAGERVGVLLCGANTTAVNF

Sequences:

>Translated_313_residues
MNLDILGMREGISRTYSVIRPFIRRTPVVDIDAGDFALPAAPLVLKLELLQHTGSFKTRGAVSNLLTRTVPPAGVVAASG
GNHGVAVAYAAMKAGKPARIFIPTISSPAKIDRIRSYGADLVVTGDRYHDALLASQEWAAQSGAMQIHAYDQVETLLGAG
SVGLEFEEQAPALDTLLVACGGGGLIGGIAGWFQGRTRVVGVEPELCPTLHRALEAGRPVDTESSGIAADSLAPNSVGAL
MFPIAQKWVERVVLVPDNAIADAQRALWNTLRVVTEPGGAAAFAAVLSRAYEPAAGERVGVLLCGANTTAVNF
>Mature_313_residues
MNLDILGMREGISRTYSVIRPFIRRTPVVDIDAGDFALPAAPLVLKLELLQHTGSFKTRGAVSNLLTRTVPPAGVVAASG
GNHGVAVAYAAMKAGKPARIFIPTISSPAKIDRIRSYGADLVVTGDRYHDALLASQEWAAQSGAMQIHAYDQVETLLGAG
SVGLEFEEQAPALDTLLVACGGGGLIGGIAGWFQGRTRVVGVEPELCPTLHRALEAGRPVDTESSGIAADSLAPNSVGAL
MFPIAQKWVERVVLVPDNAIADAQRALWNTLRVVTEPGGAAAFAAVLSRAYEPAAGERVGVLLCGANTTAVNF

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=313, Percent_Identity=28.7539936102236, Blast_Score=121, Evalue=7e-28,
Organism=Escherichia coli, GI1790207, Length=277, Percent_Identity=33.2129963898917, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1789505, Length=239, Percent_Identity=32.6359832635983, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71991565, Length=270, Percent_Identity=35.5555555555556, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17508781, Length=310, Percent_Identity=32.258064516129, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17537387, Length=276, Percent_Identity=35.5072463768116, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6322631, Length=294, Percent_Identity=29.2517006802721, Blast_Score=100, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6319788, Length=305, Percent_Identity=25.9016393442623, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320930, Length=252, Percent_Identity=30.5555555555556, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355833, Length=301, Percent_Identity=35.2159468438538, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645328, Length=215, Percent_Identity=33.4883720930233, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 32672; Mature: 32672

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDILGMREGISRTYSVIRPFIRRTPVVDIDAGDFALPAAPLVLKLELLQHTGSFKTRG
CCCEEECHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHH
AVSNLLTRTVPPAGVVAASGGNHGVAVAYAAMKAGKPARIFIPTISSPAKIDRIRSYGAD
HHHHHHHHCCCCCCEEEECCCCCCEEEEEEHHHCCCCCEEEEECCCCCHHHHHHHHCCCC
LVVTGDRYHDALLASQEWAAQSGAMQIHAYDQVETLLGAGSVGLEFEEQAPALDTLLVAC
EEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCEECCCCCHHHEEEEEC
GGGGLIGGIAGWFQGRTRVVGVEPELCPTLHRALEAGRPVDTESSGIAADSLAPNSVGAL
CCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
MFPIAQKWVERVVLVPDNAIADAQRALWNTLRVVTEPGGAAAFAAVLSRAYEPAAGERVG
HHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCEEE
VLLCGANTTAVNF
EEEECCCCCEECC
>Mature Secondary Structure
MNLDILGMREGISRTYSVIRPFIRRTPVVDIDAGDFALPAAPLVLKLELLQHTGSFKTRG
CCCEEECHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHH
AVSNLLTRTVPPAGVVAASGGNHGVAVAYAAMKAGKPARIFIPTISSPAKIDRIRSYGAD
HHHHHHHHCCCCCCEEEECCCCCCEEEEEEHHHCCCCCEEEEECCCCCHHHHHHHHCCCC
LVVTGDRYHDALLASQEWAAQSGAMQIHAYDQVETLLGAGSVGLEFEEQAPALDTLLVAC
EEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCEECCCCCHHHEEEEEC
GGGGLIGGIAGWFQGRTRVVGVEPELCPTLHRALEAGRPVDTESSGIAADSLAPNSVGAL
CCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHH
MFPIAQKWVERVVLVPDNAIADAQRALWNTLRVVTEPGGAAAFAAVLSRAYEPAAGERVG
HHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHCCCCCCCEEE
VLLCGANTTAVNF
EEEECCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} 40 {L-Ser}} 5 {L-Ser}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]