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The map label for this gene is hupB [H]

Identifier: 116625607

GI number: 116625607

Start: 8218672

End: 8220468

Strand: Direct

Name: hupB [H]

Synonym: Acid_6556

Alternate gene names: 116625607

Gene position: 8218672-8220468 (Clockwise)

Preceding gene: 116625606

Following gene: 116625608

Centisome position: 82.47

GC content: 60.16

Gene sequence:

>1797_bases
ATGGCTACGGTTAGTCAGCCCGGAAGCGAGGTACGCAACCGGGAACGCAGATTAGTGGAAATGTCGTGGGACCCGATTAC
CCGTATTGTCGGGAGCCTGGGAATTTACACCAAAATCGATTTTGCCAATCGCGAGGTGGCGGAATGCCACAGCACATCCT
CTATCTTCCGGGGCTACAGCATTTTCATGAAGGGGAAGGATCCGCGCGACGCCCACTTCATCACCAGCAGGATCTGCGGG
ATCTGCGGAGACAATCACGCCACGTGCGCCACGTACGCGCAAAACATGGCGTTCGGGATTCGTCCACCGGATATGGCGGA
GTGGATCGTCAATCTGGGCGAGGCCGCGGAGTACATGTTCGACCACAACATTTTCCAGGACAACCTGGTGGGCGTGGATT
TCTGCGAGCAGATGGTGAAGGAGACCAACCCCGGCGTTCTGGATAAGGCGGAGCGGAGCGATTGTCCGCACGCGGAGCAG
CACGGCTACCGCAAGATCGCCGACATCATGCGCGCCCTGAACCCGTTCACGGGCGAGTTCTATCGCGAGGCTCTACAGAT
GAGCCGGATGACGCGCGAGATGTTCTGCCTGATGGAAGGGCGGCACGTGCATCCTTCAACGCTGTATCCGGGAGGAGTGG
GAACGGTGCCCACCGTCCAGCTGTTCACAGATTATCTGGTGCGGCTGATGAAGTATGTCGAGTTCATGAAGCGGGTCGTG
CCCCTGCATGATGACCTATTCGACTTTTTCTATGAGGCGCTGCCGGGCTATGAGAAAGTGGGGCAGCGGCGCGTACTGCT
GGGGTGCTGGGGATCGTGGAACAATCCGGAGGTCTGCGATTACACCTACAAGAACATGAATGCGTGGGGCAACGCGATGT
ACGTAACTCCGGGCGTAGTGGTGGATGGCAAGCTGGTGACCACGAACCTGGTAGATATCAACCTGGGGATCCGTATCCTG
CTGGGGAGTTCGTTCTACGACGACTGGCAGCAGGGGGAGACCTTCGTGAAGCAGGACCCCCTCGGGAACCCGGTGGACCA
GCGCCATCCGTGGAACCAGACCACCACGCCCCGGCCGCAGAAGCGCAATTTCGATCAACGGTACACGTGGGTGATGTCGC
CCCGTTGGCACCATCCGCACACGGGCGACGATCTCGCGCTCGATACCGGCGGCGGTCCGTTGGCACGATTGTGGGCCACA
GCCTTGGCGGGCAAGGTAGACATCGGCTACATCAAGGCGACCGGGCAGAGCGTGAAGATCTATCTGCCGAAAACCCTCAA
CAAGCCCGATGCCGAGTTCGAATGGAAGATTCCGCGATGGAGCAACGCGATTGAGCGGGACCGCGCGCGTACTTACTTCC
AGGCATACGCAGCTGCGGCGGCCCTGCACTTCATGGAAAAGGGCATGGCGGACCTGCATGCCGGCAAAACAAAAACGTGG
ACGGATTTCAAGGTTCCCGAGGAAGCGATTTCGTGCGGCTTCCACGAGGCGGTTCGCGGCGTGCTCTCGCACCACGTGGT
GATCCGCAATGGGAAGATCGCCAACTACCATCCGTATCCGCCGACACCGTGGAACGCCAGTCCGCGCGATTCCAACGGGA
CGCCGGGACCGTATGAAGATGCGGTGCAGAATACGCCCATCTTCGAAGAGAACGGGCCTGAGAATTTCCGCGGGATCGAC
ATCATGCGCGCCGTGCGAAGCTTCGACCCGTGCCTGCCGTGCGGCGTGCACATGTACCTGGGCAAGGGAAAGGTGCTCGA
GACGCGCCACTCTCCGATGTTCGGGGCGCAGGGCTAG

Upstream 100 bases:

>100_bases
ACGACACGGCCGATAAAGAGACTAAGTGGCGGCATAAGCGGCCCGTGCTAACAACGGGATATAGGCCGGCAACATATAGT
TAGGACCCAGGAGGGGTAAA

Downstream 100 bases:

>100_bases
CCGCATGGAGAATGCACAGGAGATCGAACGGCTGATCGCGGAGATCGAGGCGTCGGCCGACCCGGCGACGCGGACTGGCG
TGCGGCAATTGGTGGAGGCG

Product: hydrogen:quinone oxidoreductase

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MATVSQPGSEVRNRERRLVEMSWDPITRIVGSLGIYTKIDFANREVAECHSTSSIFRGYSIFMKGKDPRDAHFITSRICG
ICGDNHATCATYAQNMAFGIRPPDMAEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEQMVKETNPGVLDKAERSDCPHAEQ
HGYRKIADIMRALNPFTGEFYREALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLVRLMKYVEFMKRVV
PLHDDLFDFFYEALPGYEKVGQRRVLLGCWGSWNNPEVCDYTYKNMNAWGNAMYVTPGVVVDGKLVTTNLVDINLGIRIL
LGSSFYDDWQQGETFVKQDPLGNPVDQRHPWNQTTTPRPQKRNFDQRYTWVMSPRWHHPHTGDDLALDTGGGPLARLWAT
ALAGKVDIGYIKATGQSVKIYLPKTLNKPDAEFEWKIPRWSNAIERDRARTYFQAYAAAAALHFMEKGMADLHAGKTKTW
TDFKVPEEAISCGFHEAVRGVLSHHVVIRNGKIANYHPYPPTPWNASPRDSNGTPGPYEDAVQNTPIFEENGPENFRGID
IMRAVRSFDPCLPCGVHMYLGKGKVLETRHSPMFGAQG

Sequences:

>Translated_598_residues
MATVSQPGSEVRNRERRLVEMSWDPITRIVGSLGIYTKIDFANREVAECHSTSSIFRGYSIFMKGKDPRDAHFITSRICG
ICGDNHATCATYAQNMAFGIRPPDMAEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEQMVKETNPGVLDKAERSDCPHAEQ
HGYRKIADIMRALNPFTGEFYREALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLVRLMKYVEFMKRVV
PLHDDLFDFFYEALPGYEKVGQRRVLLGCWGSWNNPEVCDYTYKNMNAWGNAMYVTPGVVVDGKLVTTNLVDINLGIRIL
LGSSFYDDWQQGETFVKQDPLGNPVDQRHPWNQTTTPRPQKRNFDQRYTWVMSPRWHHPHTGDDLALDTGGGPLARLWAT
ALAGKVDIGYIKATGQSVKIYLPKTLNKPDAEFEWKIPRWSNAIERDRARTYFQAYAAAAALHFMEKGMADLHAGKTKTW
TDFKVPEEAISCGFHEAVRGVLSHHVVIRNGKIANYHPYPPTPWNASPRDSNGTPGPYEDAVQNTPIFEENGPENFRGID
IMRAVRSFDPCLPCGVHMYLGKGKVLETRHSPMFGAQG
>Mature_597_residues
ATVSQPGSEVRNRERRLVEMSWDPITRIVGSLGIYTKIDFANREVAECHSTSSIFRGYSIFMKGKDPRDAHFITSRICGI
CGDNHATCATYAQNMAFGIRPPDMAEWIVNLGEAAEYMFDHNIFQDNLVGVDFCEQMVKETNPGVLDKAERSDCPHAEQH
GYRKIADIMRALNPFTGEFYREALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLVRLMKYVEFMKRVVP
LHDDLFDFFYEALPGYEKVGQRRVLLGCWGSWNNPEVCDYTYKNMNAWGNAMYVTPGVVVDGKLVTTNLVDINLGIRILL
GSSFYDDWQQGETFVKQDPLGNPVDQRHPWNQTTTPRPQKRNFDQRYTWVMSPRWHHPHTGDDLALDTGGGPLARLWATA
LAGKVDIGYIKATGQSVKIYLPKTLNKPDAEFEWKIPRWSNAIERDRARTYFQAYAAAAALHFMEKGMADLHAGKTKTWT
DFKVPEEAISCGFHEAVRGVLSHHVVIRNGKIANYHPYPPTPWNASPRDSNGTPGPYEDAVQNTPIFEENGPENFRGIDI
MRAVRSFDPCLPCGVHMYLGKGKVLETRHSPMFGAQG

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=633, Percent_Identity=27.6461295418641, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789368, Length=608, Percent_Identity=25.1644736842105, Blast_Score=148, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 67800; Mature: 67669

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATVSQPGSEVRNRERRLVEMSWDPITRIVGSLGIYTKIDFANREVAECHSTSSIFRGYS
CCCCCCCCHHHHHHHHHEEECCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEE
IFMKGKDPRDAHFITSRICGICGDNHATCATYAQNMAFGIRPPDMAEWIVNLGEAAEYMF
EEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DHNIFQDNLVGVDFCEQMVKETNPGVLDKAERSDCPHAEQHGYRKIADIMRALNPFTGEF
HCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
YREALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLVRLMKYVEFMKRVV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PLHDDLFDFFYEALPGYEKVGQRRVLLGCWGSWNNPEVCDYTYKNMNAWGNAMYVTPGVV
CHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCEEHHCCCCCCCEEEECCCEE
VDGKLVTTNLVDINLGIRILLGSSFYDDWQQGETFVKQDPLGNPVDQRHPWNQTTTPRPQ
ECCEEEEEEEEEECCCEEEEECCCHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCC
KRNFDQRYTWVMSPRWHHPHTGDDLALDTGGGPLARLWATALAGKVDIGYIKATGQSVKI
CCCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCEEEE
YLPKTLNKPDAEFEWKIPRWSNAIERDRARTYFQAYAAAAALHFMEKGMADLHAGKTKTW
EECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TDFKVPEEAISCGFHEAVRGVLSHHVVIRNGKIANYHPYPPTPWNASPRDSNGTPGPYED
CCCCCCHHHHHCCHHHHHHHHHHCCEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCHHH
AVQNTPIFEENGPENFRGIDIMRAVRSFDPCLPCGVHMYLGKGKVLETRHSPMFGAQG
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
ATVSQPGSEVRNRERRLVEMSWDPITRIVGSLGIYTKIDFANREVAECHSTSSIFRGYS
CCCCCCCHHHHHHHHHEEECCCCHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCEE
IFMKGKDPRDAHFITSRICGICGDNHATCATYAQNMAFGIRPPDMAEWIVNLGEAAEYMF
EEECCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
DHNIFQDNLVGVDFCEQMVKETNPGVLDKAERSDCPHAEQHGYRKIADIMRALNPFTGEF
HCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHH
YREALQMSRMTREMFCLMEGRHVHPSTLYPGGVGTVPTVQLFTDYLVRLMKYVEFMKRVV
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
PLHDDLFDFFYEALPGYEKVGQRRVLLGCWGSWNNPEVCDYTYKNMNAWGNAMYVTPGVV
CHHHHHHHHHHHHCCCHHHHCCCEEEEEECCCCCCCCCCCEEHHCCCCCCCEEEECCCEE
VDGKLVTTNLVDINLGIRILLGSSFYDDWQQGETFVKQDPLGNPVDQRHPWNQTTTPRPQ
ECCEEEEEEEEEECCCEEEEECCCHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCCC
KRNFDQRYTWVMSPRWHHPHTGDDLALDTGGGPLARLWATALAGKVDIGYIKATGQSVKI
CCCCCCCEEEEECCCCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCEEEEEEECCCEEEE
YLPKTLNKPDAEFEWKIPRWSNAIERDRARTYFQAYAAAAALHFMEKGMADLHAGKTKTW
EECCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
TDFKVPEEAISCGFHEAVRGVLSHHVVIRNGKIANYHPYPPTPWNASPRDSNGTPGPYED
CCCCCCHHHHHCCHHHHHHHHHHCCEEEECCEECCCCCCCCCCCCCCCCCCCCCCCCHHH
AVQNTPIFEENGPENFRGIDIMRAVRSFDPCLPCGVHMYLGKGKVLETRHSPMFGAQG
HHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2402452; 2103457 [H]