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The map label for this gene is hyb0 [C]

Identifier: 116625606

GI number: 116625606

Start: 8217587

End: 8218654

Strand: Direct

Name: hyb0 [C]

Synonym: Acid_6555

Alternate gene names: 116625606

Gene position: 8217587-8218654 (Clockwise)

Preceding gene: 116625605

Following gene: 116625607

Centisome position: 82.46

GC content: 61.14

Gene sequence:

>1068_bases
ATGGCGGCAGACACGCTTCCATATGGCCGCAAAACGCAGCGTCCTCCGGCGGTAAAAGAAGTCCACGTCCTGTGGATCAC
CGCAGGCCTAAGTTGCGACGGCGATTCCGTCTCCGTTACCGCGGCAACTCAACCCAGCATCGAAGATGTTCTCCTGGGGG
CGATCCCGGGGCTTCCCAAGGTCCACTTACACAACCCCGTGCTTGCCTATGAGGTCGGCGACGACTTCATGAAGTATTGG
TATGAAGCGGAGGCGGGAAGGCTCGAGCCGTTCGTGCTGGTAGTGGAGGGATCGATCCCCAACGAGAAGATTAAACGCGA
GGGCTACTGGGCGGCGCTGGCGACGGACCCGGCGACCGGGCAGCCGATTACCACGTGCGAATGGATCGACCGGCTCGCGC
CCAAAGCCTTGGCCGTAGTGGGTGCGGGAACGTGCGCCACTTACGGCGGCATTCACGCGATGCAGGGCAATCCCACGGGA
TGCATGGGGCTGGCGGATTACCTTGGCTGGAATTGGAAGTCCAAAGCAGGCCTGCCGATCGTGAACGTGCCGGGCTGTCC
CGTACAGCCCGATAACTTCATGGAGACGCTGCTGTATCTGCTTTATCAGGTTGCCGGCCTGGCTCCCATGATCCCGCTCG
ATGACCGGGGCCGGCCGAAGTGGCTCTTTGGAGCTACGGTGCACGAGGGCTGCGATCGCGGCGGCTACTACGAGCAGGGG
CAATTTACAAAGGAATACGGCAAGCCGGAGTGCCTGGTGCGCCTGGGCTGTTGGGGGCCGGTGGTCAATTGCAATGTGAC
GAAGCGCGGCTGGATGGCGGGAATCGGCGGCTGCCCCAACGTGGGCGGTATTTGTATCGCGTGCACGATGCCCGGGTTTC
CGGATAAGTTCATGCCGTTTATGGATGAGCCGCCGGGCGCCAAGATTTCGTCGGATATGTCCACGGCATATGGGAGAGCG
GTTCGGGCATTGCGCTCGATCACGCACGACACGGCCGATAAAGAGACTAAGTGGCGGCATAAGCGGCCCGTGCTAACAAC
GGGATATAGGCCGGCAACATATAGTTAG

Upstream 100 bases:

>100_bases
CGCGCATCTGTTAGTGGCGGGTTATATATGGCACGTTGATTTGTTGACGCCTTCCATATACACGGGCACACTGAAGAAAG
TCACGAAGAAGGAGGGGCAA

Downstream 100 bases:

>100_bases
GACCCAGGAGGGGTAAAATGGCTACGGTTAGTCAGCCCGGAAGCGAGGTACGCAACCGGGAACGCAGATTAGTGGAAATG
TCGTGGGACCCGATTACCCG

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: NA

Alternate protein names: NiFeSe hydrogenlyase small chain [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MAADTLPYGRKTQRPPAVKEVHVLWITAGLSCDGDSVSVTAATQPSIEDVLLGAIPGLPKVHLHNPVLAYEVGDDFMKYW
YEAEAGRLEPFVLVVEGSIPNEKIKREGYWAALATDPATGQPITTCEWIDRLAPKALAVVGAGTCATYGGIHAMQGNPTG
CMGLADYLGWNWKSKAGLPIVNVPGCPVQPDNFMETLLYLLYQVAGLAPMIPLDDRGRPKWLFGATVHEGCDRGGYYEQG
QFTKEYGKPECLVRLGCWGPVVNCNVTKRGWMAGIGGCPNVGGICIACTMPGFPDKFMPFMDEPPGAKISSDMSTAYGRA
VRALRSITHDTADKETKWRHKRPVLTTGYRPATYS

Sequences:

>Translated_355_residues
MAADTLPYGRKTQRPPAVKEVHVLWITAGLSCDGDSVSVTAATQPSIEDVLLGAIPGLPKVHLHNPVLAYEVGDDFMKYW
YEAEAGRLEPFVLVVEGSIPNEKIKREGYWAALATDPATGQPITTCEWIDRLAPKALAVVGAGTCATYGGIHAMQGNPTG
CMGLADYLGWNWKSKAGLPIVNVPGCPVQPDNFMETLLYLLYQVAGLAPMIPLDDRGRPKWLFGATVHEGCDRGGYYEQG
QFTKEYGKPECLVRLGCWGPVVNCNVTKRGWMAGIGGCPNVGGICIACTMPGFPDKFMPFMDEPPGAKISSDMSTAYGRA
VRALRSITHDTADKETKWRHKRPVLTTGYRPATYS
>Mature_354_residues
AADTLPYGRKTQRPPAVKEVHVLWITAGLSCDGDSVSVTAATQPSIEDVLLGAIPGLPKVHLHNPVLAYEVGDDFMKYWY
EAEAGRLEPFVLVVEGSIPNEKIKREGYWAALATDPATGQPITTCEWIDRLAPKALAVVGAGTCATYGGIHAMQGNPTGC
MGLADYLGWNWKSKAGLPIVNVPGCPVQPDNFMETLLYLLYQVAGLAPMIPLDDRGRPKWLFGATVHEGCDRGGYYEQGQ
FTKEYGKPECLVRLGCWGPVVNCNVTKRGWMAGIGGCPNVGGICIACTMPGFPDKFMPFMDEPPGAKISSDMSTAYGRAV
RALRSITHDTADKETKWRHKRPVLTTGYRPATYS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789371, Length=290, Percent_Identity=32.0689655172414, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787206, Length=319, Percent_Identity=25.0783699059561, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 38527; Mature: 38395

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADTLPYGRKTQRPPAVKEVHVLWITAGLSCDGDSVSVTAATQPSIEDVLLGAIPGLPK
CCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHCCCCCE
VHLHNPVLAYEVGDDFMKYWYEAEAGRLEPFVLVVEGSIPNEKIKREGYWAALATDPATG
EEECCCEEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHCCCEEEEEECCCCCC
QPITTCEWIDRLAPKALAVVGAGTCATYGGIHAMQGNPTGCMGLADYLGWNWKSKAGLPI
CCCHHHHHHHHHCCHHEEEEECCHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCE
VNVPGCPVQPDNFMETLLYLLYQVAGLAPMIPLDDRGRPKWLFGATVHEGCDRGGYYEQG
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCC
QFTKEYGKPECLVRLGCWGPVVNCNVTKRGWMAGIGGCPNVGGICIACTMPGFPDKFMPF
CHHHHHCCCEEEEEEECCCCEEECCCCCCCCEECCCCCCCCCCEEEEEECCCCCHHHCCC
MDEPPGAKISSDMSTAYGRAVRALRSITHDTADKETKWRHKRPVLTTGYRPATYS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure 
AADTLPYGRKTQRPPAVKEVHVLWITAGLSCDGDSVSVTAATQPSIEDVLLGAIPGLPK
CCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEEEEECCCCCHHHHHHHHCCCCCE
VHLHNPVLAYEVGDDFMKYWYEAEAGRLEPFVLVVEGSIPNEKIKREGYWAALATDPATG
EEECCCEEEEECCHHHHHHHHHCCCCCCCEEEEEEECCCCHHHHHCCCEEEEEECCCCCC
QPITTCEWIDRLAPKALAVVGAGTCATYGGIHAMQGNPTGCMGLADYLGWNWKSKAGLPI
CCCHHHHHHHHHCCHHEEEEECCHHHHHCCEEEECCCCCCHHHHHHHHCCCCCCCCCCCE
VNVPGCPVQPDNFMETLLYLLYQVAGLAPMIPLDDRGRPKWLFGATVHEGCDRGGYYEQG
EECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCC
QFTKEYGKPECLVRLGCWGPVVNCNVTKRGWMAGIGGCPNVGGICIACTMPGFPDKFMPF
CHHHHHCCCEEEEEEECCCCEEECCCCCCCCEECCCCCCCCCCEEEEEECCCCCHHHCCC
MDEPPGAKISSDMSTAYGRAVRALRSITHDTADKETKWRHKRPVLTTGYRPATYS
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3316183; 3322275; 10378275 [H]