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The map label for this gene is tpiA

Identifier: 116625555

GI number: 116625555

Start: 8150447

End: 8151196

Strand: Reverse

Name: tpiA

Synonym: Acid_6502

Alternate gene names: 116625555

Gene position: 8151196-8150447 (Counterclockwise)

Preceding gene: 116625556

Following gene: 116625552

Centisome position: 81.79

GC content: 63.33

Gene sequence:

>750_bases
ATGAGAAAACCAGTCATGGCGGGCAATTGGAAGATGTACAAAACGCCCGCGGAAACCATTGCGTTCTTCGAGAAATTCCG
GCCGCTGGTCGAGAAGTCCTCGCACTGTGAGATCGTGATCTGCCCTCCCTTCACGAACCTCGCCGCGGCCGTCGATGCCG
TGAGAGGCACGTCGATCCGGGTCGGCGCGCAGAATGTCGGCTGGGCCAAGGAAGGCGCCTTCACGGGCGAGGTTTCGGGC
CCGATGCTCAATGCCATGGGCGTCAGTCACGTCATCATCGGCCACAGCGAACGCCGCCAATATTTCAACGAGACCGATGA
GACCGTGCTGAAGCGGACTCAGGCCGCGCTCGAATTCGGGCTGACGCCGATTGTCTGCGTCGGCGAACTACTGGCCGACC
GCGAAGGCGGGAGGACCGAAGCCGTGCTCGCCGGACAGTTCCAGAAGGGCATCGCCGGATTGACGGAGCAGCAGTTCGCG
AAGATCGTGATCGCCTATGAGCCCGTGTGGGCCATCGGTACCGGCAAGACCGCGACGCCCGAGATCGCGGCCGATACGCA
CCGCGCGATCCGCGGGCAGGTCCGCGAGAAGTTCGGTAAGGAAGCGGCCGACGCGGTCCGCATTCTGTATGGCGGCAGCG
TGAAGCCCGATAACGCAAAGGTCCTGATGGGGCAGCCGGAAATCGACGGCGTCCTGGTGGGCGGCGCGGCGCTGGACCCG
GTGTCGTTCGCTTCGATCGTTAACTTCTAA

Upstream 100 bases:

>100_bases
ATTTGGGAGTGCGCGGATCGCTGAGCGATATCGGCCAGACGGTGGCCGAGAACTTCGGCGCGCGGATCGCGCAGGGACAA
AGCTTTTTATCACAACTATT

Downstream 100 bases:

>100_bases
TCTATTTCGCGGAGACTTCCACGGGGAGCTCCGCGTGGGCGCTCCACTCATCCCAGGAGCCGTCGTACATGCGGGCGTCG
TAGCCCAGGTACCGGGCGGC

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRKPVMAGNWKMYKTPAETIAFFEKFRPLVEKSSHCEIVICPPFTNLAAAVDAVRGTSIRVGAQNVGWAKEGAFTGEVSG
PMLNAMGVSHVIIGHSERRQYFNETDETVLKRTQAALEFGLTPIVCVGELLADREGGRTEAVLAGQFQKGIAGLTEQQFA
KIVIAYEPVWAIGTGKTATPEIAADTHRAIRGQVREKFGKEAADAVRILYGGSVKPDNAKVLMGQPEIDGVLVGGAALDP
VSFASIVNF

Sequences:

>Translated_249_residues
MRKPVMAGNWKMYKTPAETIAFFEKFRPLVEKSSHCEIVICPPFTNLAAAVDAVRGTSIRVGAQNVGWAKEGAFTGEVSG
PMLNAMGVSHVIIGHSERRQYFNETDETVLKRTQAALEFGLTPIVCVGELLADREGGRTEAVLAGQFQKGIAGLTEQQFA
KIVIAYEPVWAIGTGKTATPEIAADTHRAIRGQVREKFGKEAADAVRILYGGSVKPDNAKVLMGQPEIDGVLVGGAALDP
VSFASIVNF
>Mature_249_residues
MRKPVMAGNWKMYKTPAETIAFFEKFRPLVEKSSHCEIVICPPFTNLAAAVDAVRGTSIRVGAQNVGWAKEGAFTGEVSG
PMLNAMGVSHVIIGHSERRQYFNETDETVLKRTQAALEFGLTPIVCVGELLADREGGRTEAVLAGQFQKGIAGLTEQQFA
KIVIAYEPVWAIGTGKTATPEIAADTHRAIRGQVREKFGKEAADAVRILYGGSVKPDNAKVLMGQPEIDGVLVGGAALDP
VSFASIVNF

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=253, Percent_Identity=40.7114624505929, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI226529917, Length=253, Percent_Identity=40.7114624505929, Blast_Score=169, Evalue=2e-42,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=43.7246963562753, Blast_Score=196, Evalue=2e-51,
Organism=Caenorhabditis elegans, GI17536593, Length=251, Percent_Identity=43.8247011952191, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=41.1290322580645, Blast_Score=177, Evalue=1e-45,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=40.8906882591093, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=40.8906882591093, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI28572004, Length=247, Percent_Identity=40.8906882591093, Blast_Score=169, Evalue=1e-42,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_SOLUE (Q01SE4)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_827711.1
- ProteinModelPortal:   Q01SE4
- SMR:   Q01SE4
- STRING:   Q01SE4
- GeneID:   4425356
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_6502
- NMPDR:   fig|234267.9.peg.6177
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q01SE4
- ProtClustDB:   PRK00042
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26718; Mature: 26718

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 95-95 ACT_SITE 167-167 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKPVMAGNWKMYKTPAETIAFFEKFRPLVEKSSHCEIVICPPFTNLAAAVDAVRGTSIR
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCEEE
VGAQNVGWAKEGAFTGEVSGPMLNAMGVSHVIIGHSERRQYFNETDETVLKRTQAALEFG
ECCCCCCCCCCCCCCCCCCCCEEHHCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHC
LTPIVCVGELLADREGGRTEAVLAGQFQKGIAGLTEQQFAKIVIAYEPVWAIGTGKTATP
CCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCHHHHEEEEEEECCEEEECCCCCCCC
EIAADTHRAIRGQVREKFGKEAADAVRILYGGSVKPDNAKVLMGQPEIDGVLVGGAALDP
CHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCCCCEEEECCCCCCEEEECCCCCCH
VSFASIVNF
HHHHHHHCC
>Mature Secondary Structure
MRKPVMAGNWKMYKTPAETIAFFEKFRPLVEKSSHCEIVICPPFTNLAAAVDAVRGTSIR
CCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHCCCEEE
VGAQNVGWAKEGAFTGEVSGPMLNAMGVSHVIIGHSERRQYFNETDETVLKRTQAALEFG
ECCCCCCCCCCCCCCCCCCCCEEHHCCCEEEEECCHHHHHHHCCHHHHHHHHHHHHHHHC
LTPIVCVGELLADREGGRTEAVLAGQFQKGIAGLTEQQFAKIVIAYEPVWAIGTGKTATP
CCHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCHHHHEEEEEEECCEEEECCCCCCCC
EIAADTHRAIRGQVREKFGKEAADAVRILYGGSVKPDNAKVLMGQPEIDGVLVGGAALDP
CHHHHHHHHHHHHHHHHHCHHHHHHEEEEECCCCCCCCCEEEECCCCCCEEEECCCCCCH
VSFASIVNF
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA