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The map label for this gene is cysA [H]

Identifier: 116625554

GI number: 116625554

Start: 8149525

End: 8150445

Strand: Direct

Name: cysA [H]

Synonym: Acid_6501

Alternate gene names: 116625554

Gene position: 8149525-8150445 (Clockwise)

Preceding gene: 116625553

Following gene: 116625558

Centisome position: 81.78

GC content: 61.56

Gene sequence:

>921_bases
ATGAAACGAACCTGGATCGGACTAGTTCTTAGCTGCCTGATGGCGGCACACACAATTGCCGCGGCCACCTGCGGCGGCCA
TGGCGACCGCGCCACCATGCTGGTCACTACCGGCTGGCTGGCGCAACATCTCAAAGACAAGAACATGGTCATCCTGGCGA
TTGGGCAGGAGGCGGAATACAAGGAGCACATCCCGGGCGCCATCTCGCTGCGGCTGGATGAGGTCACCACGCCGATGGAA
ATGGGCAAGCTGATGTACGAACTGCCGCCGATCGATCAACTGCAAAAGACGCTCACCGCGCTCGGAATCACGAACGATTC
ACGCGTCGTTCTCTACCTGAGCAACCAGGGATTGGCGGCCATGACGCGCGTCTACCTGACGCTGGACGCCATCGGCCTGG
GAGCGCGCACTTCGATTCTCGACGGCGGCCTCAAGGTTTGGAAGAGCGAGAACCGCCCCGTGACCGCTGAGGTCGCCGCC
GTCAAACCGGGGAAATTGGATCTCTGCCCGCAGGGCGATGTGATCGCGCTCATGGACTTCGTGAAGTCCAACATCAACCA
CCCCGGCGTCAGAATTCTGGACGCGCGCGCCGAGCAGTTCTACACCGGGCAAACCGCGGGCAAGACCCACGACGGCCAGG
ATCAACGCAAGGGACACATCCCCGGTGCCGGCAATTTGCGATTCAGCTCGCTCTACGACGAGCAGGGCAAATTCAATTCA
CCCGATAAGCTGAAAGCCCAATTCACCGAAGCCGGCGTGAAGCCCGGCGACAAAGTAGTCTCGTACTGCCACATCGGCCA
GCAGGCCACGGTAGTCTACTTTGCCGCCCGGTACCTGGGCTACGACGCCCGCATGTACGACGGCTCCTGGGATGAGTGGA
GCGCCCACGCGGAGCTCCCCGTGGAAGTCTCCGCGAAATAG

Upstream 100 bases:

>100_bases
GTCTAGATCTGTGACCAACGGCCCAGGGGCAACCCTGGGCCGTTTTTCTTTACATCTCAGCGATTGCGAACTACGTCGTC
TAGCGATATAGTTGAATGGC

Downstream 100 bases:

>100_bases
ATTAGAAGTTAACGATCGAAGCGAACGACACCGGGTCCAGCGCCGCGCCGCCCACCAGGACGCCGTCGATTTCCGGCTGC
CCCATCAGGACCTTTGCGTT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: Rhodanese-like protein [H]

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MKRTWIGLVLSCLMAAHTIAAATCGGHGDRATMLVTTGWLAQHLKDKNMVILAIGQEAEYKEHIPGAISLRLDEVTTPME
MGKLMYELPPIDQLQKTLTALGITNDSRVVLYLSNQGLAAMTRVYLTLDAIGLGARTSILDGGLKVWKSENRPVTAEVAA
VKPGKLDLCPQGDVIALMDFVKSNINHPGVRILDARAEQFYTGQTAGKTHDGQDQRKGHIPGAGNLRFSSLYDEQGKFNS
PDKLKAQFTEAGVKPGDKVVSYCHIGQQATVVYFAARYLGYDARMYDGSWDEWSAHAELPVEVSAK

Sequences:

>Translated_306_residues
MKRTWIGLVLSCLMAAHTIAAATCGGHGDRATMLVTTGWLAQHLKDKNMVILAIGQEAEYKEHIPGAISLRLDEVTTPME
MGKLMYELPPIDQLQKTLTALGITNDSRVVLYLSNQGLAAMTRVYLTLDAIGLGARTSILDGGLKVWKSENRPVTAEVAA
VKPGKLDLCPQGDVIALMDFVKSNINHPGVRILDARAEQFYTGQTAGKTHDGQDQRKGHIPGAGNLRFSSLYDEQGKFNS
PDKLKAQFTEAGVKPGDKVVSYCHIGQQATVVYFAARYLGYDARMYDGSWDEWSAHAELPVEVSAK
>Mature_306_residues
MKRTWIGLVLSCLMAAHTIAAATCGGHGDRATMLVTTGWLAQHLKDKNMVILAIGQEAEYKEHIPGAISLRLDEVTTPME
MGKLMYELPPIDQLQKTLTALGITNDSRVVLYLSNQGLAAMTRVYLTLDAIGLGARTSILDGGLKVWKSENRPVTAEVAA
VKPGKLDLCPQGDVIALMDFVKSNINHPGVRILDARAEQFYTGQTAGKTHDGQDQRKGHIPGAGNLRFSSLYDEQGKFNS
PDKLKAQFTEAGVKPGDKVVSYCHIGQQATVVYFAARYLGYDARMYDGSWDEWSAHAELPVEVSAK

Specific function: May be a sulfotransferase involved in the formation of thiosulfate [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=283, Percent_Identity=29.3286219081272, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI61835204, Length=283, Percent_Identity=29.3286219081272, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI194473681, Length=283, Percent_Identity=29.3286219081272, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI17402865, Length=290, Percent_Identity=28.2758620689655, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI87082121, Length=283, Percent_Identity=27.5618374558304, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI87081967, Length=249, Percent_Identity=25.7028112449799, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71997283, Length=246, Percent_Identity=31.7073170731707, Blast_Score=104, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17561888, Length=247, Percent_Identity=29.9595141700405, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115534702, Length=213, Percent_Identity=26.7605633802817, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 33376; Mature: 33376

Theoretical pI: Translated: 7.28; Mature: 7.28

Prosite motif: PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTWIGLVLSCLMAAHTIAAATCGGHGDRATMLVTTGWLAQHLKDKNMVILAIGQEAEY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCCCEEEEEECCCCCH
KEHIPGAISLRLDEVTTPMEMGKLMYELPPIDQLQKTLTALGITNDSRVVLYLSNQGLAA
HHHCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
MTRVYLTLDAIGLGARTSILDGGLKVWKSENRPVTAEVAAVKPGKLDLCPQGDVIALMDF
HHHHHHEEEHHCCCCHHHHHHCCHHEEECCCCCEEEEEEEECCCCEECCCCCCHHHHHHH
VKSNINHPGVRILDARAEQFYTGQTAGKTHDGQDQRKGHIPGAGNLRFSSLYDEQGKFNS
HHHCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCC
PDKLKAQFTEAGVKPGDKVVSYCHIGQQATVVYFAARYLGYDARMYDGSWDEWSAHAELP
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
VEVSAK
EEECCC
>Mature Secondary Structure
MKRTWIGLVLSCLMAAHTIAAATCGGHGDRATMLVTTGWLAQHLKDKNMVILAIGQEAEY
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHCCCCCEEEEEECCCCCH
KEHIPGAISLRLDEVTTPMEMGKLMYELPPIDQLQKTLTALGITNDSRVVLYLSNQGLAA
HHHCCCEEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHH
MTRVYLTLDAIGLGARTSILDGGLKVWKSENRPVTAEVAAVKPGKLDLCPQGDVIALMDF
HHHHHHEEEHHCCCCHHHHHHCCHHEEECCCCCEEEEEEEECCCCEECCCCCCHHHHHHH
VKSNINHPGVRILDARAEQFYTGQTAGKTHDGQDQRKGHIPGAGNLRFSSLYDEQGKFNS
HHHCCCCCCEEEEECCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHCCCCCCCCC
PDKLKAQFTEAGVKPGDKVVSYCHIGQQATVVYFAARYLGYDARMYDGSWDEWSAHAELP
CHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCC
VEVSAK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]