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The map label for this gene is bhbA [H]

Identifier: 116625525

GI number: 116625525

Start: 8112721

End: 8114313

Strand: Reverse

Name: bhbA [H]

Synonym: Acid_6472

Alternate gene names: 116625525

Gene position: 8114313-8112721 (Counterclockwise)

Preceding gene: 116625527

Following gene: 116625524

Centisome position: 81.42

GC content: 63.15

Gene sequence:

>1593_bases
ATGAAAGCCGCCGAACGTCAGTTTACGACAATTAGTGGGGTGCCGATTGAGCCGGTGTACGGCCCGGAGGATTTGCGCGA
GATCGACACCGCGCGCGAGATCGGCGAACCGGGCGAGTTCCCTTATACCCGCGGCATCCATCGCGACATGTATCGCGGCA
AAATGTGGACCATGCGCCAGTTCTCCGGCTTCGCTACGCCGGAGGAGACCAACCAGCGCTACCACTACCTGCTGGGCCAG
GGACAGACCGGACTCTCCGTCGCCTTCGATCTGCCCACCCTGATGGGCTACGACGCCGATCACCCGATGAGCGAAGGCGA
AGTCGGTAAGTGCGGCGCGCCGGTGTCGTCGCTGGAGGACATGGAGATCCTCTTCCGCGGCATTCCGCTCGGGGAAGTCA
CAGTCTCGATGACCATCAACTCTCCGGCGGCGGTGCTCTGGGCGATGTACCTGGCGGTCGCCGAGCAGCAGGGCGTGGAT
TGGGCGCGACTCTCCGGTACGCTCCAGAACGACATTCTCAAGGAGTACATCGCCCAGAAGGAGTACATCTTTCCGCCGCG
TCCTTCCATGCGACTGGTGACCGATACCATCGAATTCGGCGCGCAACACACTCCCCGGTTTAACCCAGTCTCGATCAGCG
GATATCACATCCGCGAGGCCGGCTCCACGGCTGTGCAGGAGCTCGCTTTCACGCTCCGCGACGGCATGGAGTATGTGGAT
TGGGCGCTCCAACGCGGCCTGGCGATCGACGACTTCGCCCCCCGGCTCAGCTTTTTCTTCAACGCGCACAGCGATTTCTT
CGAAGAGATCGCCAAGTATCGCGCGGCCCGCAAGATCTGGGCGCACGCCATGCGCGAGCGGTACGGCGCCAAAAGCGAGC
GCAGCCTCAAACTGCGCTTCCACGCGCAGACCGCGGGCTGCTCGCTGACCTGGCAGCAGCCATACAACAACGTGGTGCGC
ACCGCCCTGCAAGCCATGGCGGCGGTGCTGGGCGGAGCGCAGTCGCTGCACACCAATTCCCTGGACGAGGCCTACGCGTT
GCCGGGCGAGCATGCCGTCACCCTCGCGCTGCGCACCCAGCAGGTGTTGGCGTACGAGACCGGCGTGACTAATGCCGCCG
ACCCGCTCGGCGGCAGCTACTTCCTGGAGAAGTTGACCCTCCAAACCGAAGCCGCCGCTAACGACTATATCCGTCGGATC
GACGAAATGGGCGGTATGATCGCCGCCATCGAGGCCGGTTTTCCGCAGAGCGAGATCGCCAACGCGAGCTACCGTTACCA
GCGCGAAATCGAGACCGGAGAGCGCACCGTCGTGGGCGTCAACCGGTTCCAGAGCGATGACGCGCCTATCGACCTGCTCC
AGATCGATGAAACCGCCGGCCGGCGGCAGATCGCAAAACTGGAGGCGCTCCGCGCACGGCGCGACAATTGCGAGGTGACC
CGGAGTCTCGATGCTTTGAAACGTGCCGCCGAAGGAAGCGGCAACACCATGCCGCTGCTATTGGATGCCGTTCGCGCCTA
TGCCACACTGGGAGAGATCTGCGACGCGTTACGCGGTGTTTTTGGAACCTATACGGAAACTACGCATCTCTAG

Upstream 100 bases:

>100_bases
CATTTTGATGGTTTCGTTCTCAATATAGCGCGCGAGTTAACCACATCACCGTGGTGTCCGCCGCCGCGCAGGGGTTAGAA
TAACGGATAGTTGGGGACTC

Downstream 100 bases:

>100_bases
GCAGCAGGAAATGTCCCGAAAGATCATGTCACGACCGATTCGAGTACTGGTGGCGAAGCCCGGACTGGACGGGCACGACC
GCGGCGCGAAGGTGATCGCC

Product: methylmalonyl-CoA mutase

Products: NA

Alternate protein names: MCM [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MKAAERQFTTISGVPIEPVYGPEDLREIDTAREIGEPGEFPYTRGIHRDMYRGKMWTMRQFSGFATPEETNQRYHYLLGQ
GQTGLSVAFDLPTLMGYDADHPMSEGEVGKCGAPVSSLEDMEILFRGIPLGEVTVSMTINSPAAVLWAMYLAVAEQQGVD
WARLSGTLQNDILKEYIAQKEYIFPPRPSMRLVTDTIEFGAQHTPRFNPVSISGYHIREAGSTAVQELAFTLRDGMEYVD
WALQRGLAIDDFAPRLSFFFNAHSDFFEEIAKYRAARKIWAHAMRERYGAKSERSLKLRFHAQTAGCSLTWQQPYNNVVR
TALQAMAAVLGGAQSLHTNSLDEAYALPGEHAVTLALRTQQVLAYETGVTNAADPLGGSYFLEKLTLQTEAAANDYIRRI
DEMGGMIAAIEAGFPQSEIANASYRYQREIETGERTVVGVNRFQSDDAPIDLLQIDETAGRRQIAKLEALRARRDNCEVT
RSLDALKRAAEGSGNTMPLLLDAVRAYATLGEICDALRGVFGTYTETTHL

Sequences:

>Translated_530_residues
MKAAERQFTTISGVPIEPVYGPEDLREIDTAREIGEPGEFPYTRGIHRDMYRGKMWTMRQFSGFATPEETNQRYHYLLGQ
GQTGLSVAFDLPTLMGYDADHPMSEGEVGKCGAPVSSLEDMEILFRGIPLGEVTVSMTINSPAAVLWAMYLAVAEQQGVD
WARLSGTLQNDILKEYIAQKEYIFPPRPSMRLVTDTIEFGAQHTPRFNPVSISGYHIREAGSTAVQELAFTLRDGMEYVD
WALQRGLAIDDFAPRLSFFFNAHSDFFEEIAKYRAARKIWAHAMRERYGAKSERSLKLRFHAQTAGCSLTWQQPYNNVVR
TALQAMAAVLGGAQSLHTNSLDEAYALPGEHAVTLALRTQQVLAYETGVTNAADPLGGSYFLEKLTLQTEAAANDYIRRI
DEMGGMIAAIEAGFPQSEIANASYRYQREIETGERTVVGVNRFQSDDAPIDLLQIDETAGRRQIAKLEALRARRDNCEVT
RSLDALKRAAEGSGNTMPLLLDAVRAYATLGEICDALRGVFGTYTETTHL
>Mature_530_residues
MKAAERQFTTISGVPIEPVYGPEDLREIDTAREIGEPGEFPYTRGIHRDMYRGKMWTMRQFSGFATPEETNQRYHYLLGQ
GQTGLSVAFDLPTLMGYDADHPMSEGEVGKCGAPVSSLEDMEILFRGIPLGEVTVSMTINSPAAVLWAMYLAVAEQQGVD
WARLSGTLQNDILKEYIAQKEYIFPPRPSMRLVTDTIEFGAQHTPRFNPVSISGYHIREAGSTAVQELAFTLRDGMEYVD
WALQRGLAIDDFAPRLSFFFNAHSDFFEEIAKYRAARKIWAHAMRERYGAKSERSLKLRFHAQTAGCSLTWQQPYNNVVR
TALQAMAAVLGGAQSLHTNSLDEAYALPGEHAVTLALRTQQVLAYETGVTNAADPLGGSYFLEKLTLQTEAAANDYIRRI
DEMGGMIAAIEAGFPQSEIANASYRYQREIETGERTVVGVNRFQSDDAPIDLLQIDETAGRRQIAKLEALRARRDNCEVT
RSLDALKRAAEGSGNTMPLLLDAVRAYATLGEICDALRGVFGTYTETTHL

Specific function: Catalyzes the interconversion of succinyl-CoA and methylmalonyl-CoA during the polyhydroxyalkanoate degradation pathway [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=519, Percent_Identity=48.1695568400771, Blast_Score=489, Evalue=1e-138,
Organism=Escherichia coli, GI1789284, Length=517, Percent_Identity=48.1624758220503, Blast_Score=476, Evalue=1e-135,
Organism=Caenorhabditis elegans, GI25144500, Length=525, Percent_Identity=46.4761904761905, Blast_Score=496, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: =5.4.99.2 [H]

Molecular weight: Translated: 58946; Mature: 58946

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAERQFTTISGVPIEPVYGPEDLREIDTAREIGEPGEFPYTRGIHRDMYRGKMWTMRQ
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
FSGFATPEETNQRYHYLLGQGQTGLSVAFDLPTLMGYDADHPMSEGEVGKCGAPVSSLED
HCCCCCCCCCCCCEEEEEECCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
MEILFRGIPLGEVTVSMTINSPAAVLWAMYLAVAEQQGVDWARLSGTLQNDILKEYIAQK
HHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
EYIFPPRPSMRLVTDTIEFGAQHTPRFNPVSISGYHIREAGSTAVQELAFTLRDGMEYVD
CCCCCCCCCCEEEHHHHHHCCCCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHH
WALQRGLAIDDFAPRLSFFFNAHSDFFEEIAKYRAARKIWAHAMRERYGAKSERSLKLRF
HHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HAQTAGCSLTWQQPYNNVVRTALQAMAAVLGGAQSLHTNSLDEAYALPGEHAVTLALRTQ
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCCCEEEEEEEHH
QVLAYETGVTNAADPLGGSYFLEKLTLQTEAAANDYIRRIDEMGGMIAAIEAGFPQSEIA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHC
NASYRYQREIETGERTVVGVNRFQSDDAPIDLLQIDETAGRRQIAKLEALRARRDNCEVT
CCHHHHHHHHCCCCEEEEECHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
RSLDALKRAAEGSGNTMPLLLDAVRAYATLGEICDALRGVFGTYTETTHL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC
>Mature Secondary Structure
MKAAERQFTTISGVPIEPVYGPEDLREIDTAREIGEPGEFPYTRGIHRDMYRGKMWTMRQ
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCHHHHHH
FSGFATPEETNQRYHYLLGQGQTGLSVAFDLPTLMGYDADHPMSEGEVGKCGAPVSSLED
HCCCCCCCCCCCCEEEEEECCCCCCEEEEECHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
MEILFRGIPLGEVTVSMTINSPAAVLWAMYLAVAEQQGVDWARLSGTLQNDILKEYIAQK
HHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
EYIFPPRPSMRLVTDTIEFGAQHTPRFNPVSISGYHIREAGSTAVQELAFTLRDGMEYVD
CCCCCCCCCCEEEHHHHHHCCCCCCCCCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHH
WALQRGLAIDDFAPRLSFFFNAHSDFFEEIAKYRAARKIWAHAMRERYGAKSERSLKLRF
HHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
HAQTAGCSLTWQQPYNNVVRTALQAMAAVLGGAQSLHTNSLDEAYALPGEHAVTLALRTQ
EEECCCCEEEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHCCCCCCEEEEEEEHH
QVLAYETGVTNAADPLGGSYFLEKLTLQTEAAANDYIRRIDEMGGMIAAIEAGFPQSEIA
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHC
NASYRYQREIETGERTVVGVNRFQSDDAPIDLLQIDETAGRRQIAKLEALRARRDNCEVT
CCHHHHHHHHCCCCEEEEECHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHH
RSLDALKRAAEGSGNTMPLLLDAVRAYATLGEICDALRGVFGTYTETTHL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9889346; 11481431 [H]